We let our tough blocks soak in Downy fabric softener face down (depending,
10-30 minutes) and then try to section, sometimes use ice, then coat our slides
in egg whites if they are tough to adhere.
_
Jessica Riggleman |
the mineralized bone black and a basic fuschin counter
stain the osteoid will stain deep pink.
Get Outlook for iOS
From: Jessica Riggleman via Histonet
Sent: Monday, July 9, 2018 1:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New Bone
Hello,
I am trying to assess new bone vs. old bone in an animal model. Any suggestions
on thick section stains? I have heard Van Gieson's?
Thank You,
Jessica
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Hi Everyone,
I am trying to embed screws in plastic (in the hopes to see breakage in the
screw, etc). Right now I use specifically a mixture of methyl methacrylate,
poly methyl methacrylate, and benzoyl peroxide. However this usually takes a
few weeks to embed/dry. I am looking for something a
Hello,
Looking for a protocol for staining thick plastic methyl methacrylate sections
(100-150 um). Goldner’s or any trichrome stain would work.
Thank You!
Jessica
_
Jessica Riggleman | Research Associate
Globus Medical,
Hello,
Does anyone have any experience embedding umbilical cord tissue? Preferably
paraffin but we may try plastic as well. Any help/protocols/etc. would be
appreciated.
Thank You,
Jessica
_
Jessica Riggleman | Research
Hello,
I am looking for a used ultrasonic cleaning bath and a used plate shaker. If
you have either one laying around your lab please let me know! Will pay.
Thank You,
Jessica Riggleman
_
Jessica Riggleman | Research
Hi Everyone,
I am looking for a camera for a microscope (specifically for an Olympus BX41)
to look at cellular detail. If you have one that is not in use please let me
know.
Thank You,
Jessica
_
Jessica Riggleman |
Ph.D.
George Mason University
Fairfax, VA 22030-
________
From: Jessica Riggleman via Histonet <histonet@lists.utsouthwestern.edu>
Sent: Wednesday, February 8, 2017 1:07:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Looking for a Histolog
Hi Everyone,
I am looking for workshop or class for Histology/Histotechnology that lasts
anywhere from 1 week to 3 months in the U.S.
Preferably in-person and not online.
If you have any suggestions or contacts, please let me know.
Thank You,
Jessica
Hello,
Would anyone mind sharing their procedure after coverslipping a slide?
Do you let the slide sit and dry? Do you use xylenes to wipe of the extra
mounting medium immediately?
I noticed after storing the slides in a slide box, a lot of dust collects. Is
this because of the xylenes?
Hi Amanda,
We use T, they record temperature and humidity.
http://www.tandd.com/product/tr7wfnw_series.html
-Jessica
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General
Hello,
I am looking to use a Reichert-Jung Polycut S Microtome to cut MMA thin
sections (5-10 microns). However the specimens I am cutting are very small. How
do you go about gripping such small sections on such a microtome? I have tried
using a dental mount to adhere the specimen to a blank
Hi Everyone,
Please tell me your tricks of removing paraffin from containers, lab benchtops,
etc…..
I would really appreciate it!
Thank you,
Jessica
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge
Hello,
I send mine out to IMEB in California. You may be able to sell the blades on
Ebay.
Thank you,
Jessica
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead
Hello,
Could you please help me find a macrophage stain that will work with sheep
(specifically sheep laminectomy sections)? We are thinking RAM-11 or HAM-56,
but unsure.
Also, what about Rabbit PLF?
Please note both are embedded in mega cassettes for paraffin processing.
Thank you,
Jessica
Hello,
I am looking for a similar machine to the Reichert-Jung Polycut S Microtome. I
am looking to cut MMA thin sections on rabbit femoral condyles (somewhat larger
than usual specimen size). If anyone can recommend a newer microtome or is
looking to sell, that would be great. I am unaware of
Hi,
We use a Faxitron machine in our lab.
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:
Can anyone recommend a good Knife Sharpening service around the east coast? We
are located in the PA area. We have tried CL Sturkey and Dorn & Hart Microedge.
It doesn't necessarily need to be East Coast.. We are just looking for ~1 Week
turn around time.
Thank you,
Jessica
RBS is the same as Methylene Blue, I believe.
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:
Lauren,
What is your pump made out of?
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:
Hello,
Does anyone have a suggestion for a good positive control for TRAP staining?
Thank you,
Jessica Riggleman
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General
Hello,
Does anyone have an old vortex mixer they do not need, such as the one below:
http://www.ebay.com/itm/Fisher-G-560-Vortex-Genie-2-Lab-Mixer-Disc-Platform-Shaker-12-812-Ref-A-/282149263394?hash=item41b1681822:g:IwIAAOSwZVlXvu9V
I am willing to pay you and willing to pay shipping.
Thank
Also if anyone has any photos of slides stained that would be great. We are
looking for osteoclasts.
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue |
Can anyone recommend a TRAP (tartrate resistant acid phosphatase) protocol for
formalin fixed paraffin embedded Rabbit PLF specimens? Or a kit to use?
Thank you,
Jessica
_
Jessica Riggleman | Research Associate
Globus
ab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-Original Message-
From: Jessica Riggleman via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, September 12, 2016 8:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet
Hello,
We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.
We have had issues staining H and Goldner’s Trichrome. Egg whites is working
as a great slide adhesive for these two, but not so much for IHC Staining (RAM
11 specifically).
Any ideas/suggestions on how to get
Hello,
Can anyone recommend a stain that differentiates new (woven) bone vs. old
(lamellar) bone? We are looking for bone growth, and biomaterial was used to
facilitate. We are using a rabbit femoral defect and a rabbit PLF model.
Protocols/Input for MMA thin/thick sections and formalin fixed
28 matches
Mail list logo