RE: [Histonet] MAP-2 antibody

2009-05-31 Thread Jim Manavis
Robert If you are using formalin fixed paraffin embedded human material, Sigma have a very nice mouse monoclonal that works beautifully. The cat no. is M-4403 (clone HM-2). Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000

RE: [Histonet] Does anybody use PARP?

2009-02-04 Thread Jim Manavis
Alexandra I have used it and it's a very good antibody. It works extremely well in both Human and rat tissue. We use at a dilution of 1/2000 (overnight incubation) with EDTA antigen retrieval using a streptavidin detection system. Cheers Jim Jim Manavis Laboratory Manager Hanson Inst

RE: [Histonet] Neurofilament Question

2008-12-02 Thread Jim Manavis
Margaret Get on to the Covance site as they have the Strenberger Neurofilaments. Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: [EMAIL PROTECTED

RE: [Histonet] Cleaved Caspase-3

2008-12-02 Thread Jim Manavis
Tumour tissue, I use CNS Lymphoma. Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: [EMAIL PROTECTED] Disclaimer: Not this little black duck