Re: [Histonet] FW: Microtome at home

2020-04-16 Thread Joseph Saby via Histonet
You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties.  Joe Saby Sent from Yahoo Mail on Android On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy

Re: [Histonet] HSV-2 antibody - Cell Marque was not the problem

2019-07-04 Thread Joseph Saby via Histonet
Beth- I am proud of you for stepping up and correcting the record. This could not be an easy thing to do in such a public forum, but it also essential. I hope you have a wonderful holiday. Joe Saby On Wednesday, July 3, 2019, 2:33:16 PM EDT, O'Neil, Beth via Histonet wrote: Last week

Re: [Histonet] Histonet Digest, Vol 184, Issue 1

2019-03-05 Thread Joseph Saby via Histonet
The more important question is who the h*ll is Kelly Jordan. Terri has established her competency and reputation in NSH and the Histonet for probably over 20 years. You have done your company a great disservice. People will remember you, probably not as you would wish. Joe Saby, retired On Tues

Re: [Histonet] VIP issue

2018-10-11 Thread Joseph Saby via Histonet
Gudrun- Assuming this is a new issue with a recent reagent change, I suspect that the alcohol used for the clean cycle was not 100% (maybe 95%?). The ability of your alcohol to hold the paraffin in solution is lost as the percentage of water increases. I also expect your solvent was mostly satur

Re: [Histonet] broken screw in MMA

2018-06-22 Thread Joseph Saby via Histonet
Talk with the people at EXAKT technologies.  They have plastice specially designed just for samples like that. Joe On Friday, June 22, 2018, 1:47:38 PM EDT, Terri Braud via Histonet wrote: Just an idea.  Why not use SEM?  It seems like it would be so much easier. Terri L. Braud, HT(A

Re: [Histonet] Supervising a histologist

2017-11-01 Thread Joseph Saby via Histonet
Eileen- I think it would depend on what specific procedures both of them are signed off on.And it would be on specific procedures.Or things are very strange in that area of Histoland. Joe Saby From: Eileen Akemi Allison via Histonet To: Histonet Sent: Wednesday, November 1, 2017 8:

Re: [Histonet] Mammary Tissue Processing & Sectioning Troubles

2017-09-27 Thread Joseph Saby via Histonet
Andrea- I believe your tissue was not fully fixed.  Many labs think they know more than they do, so itis always a good idea to pin down exactly what they did to fix the tissue.  I am guessing they left thick blobs of tissue in an inadequate volume of formalin and expected that to do the trick.

Re: [Histonet] VIP5

2015-10-14 Thread Joseph Saby via Histonet
We had this happen when the individual changing the processor reversed order of the alcohols, placing the newest first and the oldest last.  Hope your answer is as simple, even if it is embarrassing. Joe Saby From: "Abbott, Tanya via Histonet" To: "histonet@lists.utsouthwestern.edu"