[Histonet] Flammable Sprays in Cryostats

I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? Thank you. Deanne Knutson Supervisor Anatomic Pathology

[Histonet] CPT Code 88172

Hello Histonetters! I am wondering how others may be using the cpt code 88172-T? Like in what type of scenarios? Thank you for your responses. Deanne Knutson Supervisor Anatomic Pathology dknut...@primecare.org This email

Re: [Histonet] Tissue Adherence Issue

"Let All Be Received as Christ." -Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, July 17, 2019 9:22 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Adherence Issue CAUTION: This email is not

[Histonet] Tissue Adherence Issue

Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on

[Histonet] MSH2 and MSH6 Loss of Expression

Fellow Histonet Members - We are in the process of moving our IHC bench from the Leica BOND to the ROCHE ULTRA and are in the mist of antibody validations. We are having great difficulty in finding negatives to validate for MSH2 and MSH6. Would anyone have any loss of expression MSH2 and MSH6

Re: [Histonet] Litigation Fees

"Let All Be Received as Christ." -Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, April 19, 2018 12:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Litigation Fees CAUTION: This email is not

[Histonet] Litigation Fees

Fellow histonetters, Would it be possible to hear what other histology labs are charging lawyers for when they request slides from you? For the cutting of an unstained slide? And also for the cutting and H staining of a slide? I would appreciate knowing about what amount others are charging

[Histonet] Job Opening

We have an opening for a certified Pathology Assistant to join six pathologists in a thriving anatomic pathology practice in the Northern Plains. Please feel free to contact me or visit our website at www.st.alexius.org where more information and application can be

[Histonet] (CLRW) - Clinical Laboratory Reagent Water

Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology

[Histonet] ANP.23410

Hi fellow Histonetters - I was wondering if I could get some feedback from my peers on how you are dealing with the CAP standard ANP.23410 on Cryostat Decontamination. It states that this is done at defined intervals appropriate for the institution. The place I just inspected was doing a weekly

[Histonet] HER2/NEU

I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from

[Histonet] CAP standard GEN 61300

Fellow Histonetters, I am looking for advice concerning CAP standard GEN 61300 Climate Control. To those that take humidity records in your lab, what exact % range do you use for a normal range? And what do you do if and when you are out of this range? Thank you for sharing your workflow info

[Histonet] Quality Management Monitors

In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality

[Histonet] RE: lab chairs

Amber, We recently purchased chairs from VWR International LLC and are pleased with them. They are solid black, no arm rests, have adjustments for ergonomic settings, on rollers, etc The catalog numbers are 80086-438 and 80086-440 if you want to look them up on their website. The price

[Histonet] Ventana NexES Special Stainer

We are moving our special stains bench from manual to automation with the Ventana NexES Special Stainer. I would be interested to hear from other Ventana users. How do you run your controls? Do you stain a control with each stain batch? Or one a day? I can't find any CAP standards on this.

[Histonet] 2012 CPT Codes

Hello Fellow Histonet Followers, I was wondering if anyone has had a chance to look through the 2012 pathology CPT codes yet? There is a revision on 88312, 88313, 88314, and 88319. The revision states to report one unit of the code for each special stain, on each surgical pathology block,

[Histonet] Control Blocks

Hello, Does anyone out there have positive Pneumocystis control blocks that they may want to trade with? We have some positive GRAM control blocks that we could exchange. Please let me know. Thank you so much! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center

[Histonet] IHC double stains

Fellow Histonetters, We presently use DAB brown chromagen for our IHC, and are looking at adding a red kit chromagen for staining double IHC slides. I am looking for information on what antibody scenarios other sites are using the double stain for? Kappa and lambda might be one pair of

[Histonet] p16

Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730

[Histonet] UV light in cryostats

Hello Everyone, Does anyone know if the UV lighting that can now be purchased with new cryostats will fulfill the CAP disinfection standard? Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknut...@primecare.org mailto:dknut...@primecare.org

[Histonet] Cryostats

Dear Histonetters, Does anyone know if there is some type of UV light kit that is available to be put into older model cryostats that would help meet the CAP standard for disinfecting weekly? Would appreciate hearing if there is! Deanne Knutson Anatomic Pathology Supervisor St. Alexius

[Histonet] Peloris processor

We are also interested in other's experiences with the Peloris processor. We are considering a rapid tissue processor and would like to hear from others who have rapid tissue processors. What type do you have? And please share your pros and cons. Thank you very much for your help. Deanne

[Histonet] (no subject)

Histonetters, Does anyone out there in histoland have a job description in their lab for staff that are like aides or assistants? They would be entering specimens, distributing slides, logging in placentas, tossing specimens, and just basically assisting the technical staff in any way possible.

[Histonet] Voice Recognition

Hello fellow Histonetters - I could use some advice from those who have voice recognition in place at their labs. We are having an online demo given to us soon and I could use some help on any pertinent questions that should be addressed. Are there any concerns that I should be asking about?

[Histonet] Dragon

We are currently researching the Dragon voice recognition system and I see that there is quite a bit of recent emails on the histonet concerning this subject. Is there anyone who is using Dragon that experienced reluctant Pathologists and how did you deal with that? I would be very interested in

[Histonet] (no subject)

I was wondering if anyone on the histonet uses an alcian yellow stain method for H. Pylori? Do you have a problem finding the alcian yellow, or do you use a substitute? I would be interested to hear your pros and cons on this stain. Do you stain manually or use a kit? If a kit, where do you

[Histonet] Controls needed!

We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic

[Histonet] Fix Sediment Method

Fellow Histonetters - I am looking for feedback on the alternative methods to prepare Non GYN cellblocks. We currently use the Fix Sediment Method where you spin the fluid to achieve a button, pour off the supernatant, and pour formalin onto the button to harden it. What do others do? Does

[Histonet] Specimen Handling Procedure for Breast Specimens with Predictive Markers

With the new CAP regulations concerning Her-2/neu and Predictive Markers on breast specimens, has anyone let the processed specimens sit at room temp for a length of time before embedding? We are not a 24/7 staffed lab and would appreciate any input on how others deal with these regulations.