Re: [Histonet] tissue cassettes

I am old school and prefer them dry, lol. I agree with Thomas, that shouldn't have that affect. Ginny -Original Message- From: Thomas Podawiltz via Histonet Sent: Friday, February 9, 2024 8:34 AM To: Brazie, Jeneanne E *HS ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] t

Re: [Histonet] Retic help

Same here, we moved to a new location and the PH of the water was too acidic, the DI water would remove the silver and leave it yellowed. Some solutions that I was making that usually would take hours to go into solution on the stir plate went immediately. Good Luck! Ginny Kurth, HT, (ASCP)C

Re: [Histonet] Sections sticking to tweezers

You may try to turn your water bath down a few degrees and see if this helps and what is the melting point of the paraffin you are using? Most likely a paraffin/heat situation. If I run into this issue, a quick fix is I rinse my forceps into the ice water, wipe, and the sections will not sti

Re: [Histonet] Mice/Rat brain

I remember adding a couple drops of ETOH to the water bath; it's been a while so I am not sure the exact protocol. (it will spin the tissue). Virginia Kurth, HT, (ASCP)CM UW Health Surgical Pathology Madison, WI 53792 -Original Message- From: Renee Fisher via Histonet Sent: T

[Histonet] anti-HGAL

Does anyone use HGAL on an Ultra and would be willing to share information? Thanks! Ginny Kurth, HT, (ASCP)CM Developmental Specialist UW Health Surgical Pathology Highland Ave, WIMR MB1707.6 Madison, WI 53792 vku...@uwhealth.org - __

Re: [Histonet] IHC staining of cartilage

Make sure they sit at room temperature longer than normal and then dry them longer. I found that cutting them thinner (3 microns) also helps. Ginny Kurth, HT, (ASCP)CM UW Health Surgical Pathology Highland Ave Madison, WI -Original Message- From: Hobbs, Carl via Histonet

[Histonet] ADH5

Does anyone know if there is a reference lab that performs ADH5. (particular the BioCare multiplex stain?) Thanks Ginny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Prostate whole mounts

1. As standard of care, do you process prostates as whole mounts (i.e. mega cassettes)? 2. Are there a subset of prostate resection cases that are processed in this manner (i.e. based on dx)? 3. Do you have any specimen types that are processed as whole mounts as standard of care? 4.

Re: [Histonet] Bleach Regs

I agree! Ginny Kurth Developmental specialist University Hospital of Wisconsin Surgical Pathology From: Gareth Davis via Histonet Sent: Thursday, January 31, 2019 11:52:24 AM To: Maryann Deathridge Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet

[Histonet] REPEATS IN IHC

Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too: The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH. Thanks Ginny _

[Histonet] HPV ISH

Hello I have seen question for KDL asking about HPV ISH (awhile back). My lab would like to bring on the HPV ISH using the RNA ISH probes provided by Ventana using the silver detection, are you on board using this process for HPV, do you have any insight for me. Thanks! Ginny, UWHC-Wisconsin,

[Histonet] Silver ISH

Hello Jacqueline I am going thru old histo net messages and was wondering if you had any new insight on the silver ISH for HPV testing on the Ventana ultras, my lab would like to validate it, but I am worried about the consistency of the silver staining. Thank you, Ginny Kurth, UWHC, vku...@uwhe

[Histonet] Her2 dual ISH

Hello I was reading the past posts of inconsistent her2 dual ISH results on the VMS Ultra. I am wondering if anyone has unlocked the secret of getting consistent results? Thank you advance! Ginny ___ Histonet mailing list Histonet@lists.utsouthwestern

[Histonet] PROX1

Hello Does anyone send out for Prox1? Thanks. Ginny UW-madison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] LFABP and PHH3 Abs

Hi Kiranjit I saw a question you had out on histonet back in 2015 about LFABP antibody, did anyone respond? One of our doctors want to bring this antibody on. Thanks Ginny UW Hospital of WI vku...@uwhealth.org ___ Histonet mailing list Histonet@lists.u

[Histonet] PLA 2R

Hello Does or will anyone perform PLA 2R on paraffin Renal biopsies for validation studies. We are currently sending them to a reference lab but they will not perform validation slides. Thanks ahead of time. Ginny Kurth University Hospital of Wisconsin

[Histonet] Stat Specimens

What types of specimens do other institutions deem as appropriate for STAT processing? Currently our institution allows biopsies for rejection, graft Vs host, r/o BK or CMV. Thanks for any input! Jenny University Hospital of Wisconsin ___ Histonet mai

Re: [Histonet] ATRX Antibody

Hi Pam I was wondering if any reached out to you about ATRX? Our neuropathologist is asking about it. Thanks Ginny Kurth vku...@uwhealth.org Surgical Pathology Developmental Specialist University Hospital Wisconsin ___ Histonet mailing list Histonet@

[Histonet] ATRX Ab Recommendation

Hi Dave Did you ever receive any information on the antibody ATRX? Our neuropathologist is interested. Thanks Ginny Kurth vku...@uwhealth.org Surgical Pathology Developmental Specialist University Hospital Wisconsin ___ Histonet mailing list Histone