Re: [Histonet] AFB STAIN

2018-10-04 Thread Laurie Colbert via Histonet
You should put the AFB control on a separate slide to prevent cross-contamination.  Laurie Redmond -Original Message- From: ADESUPO ADESUYI via Histonet To: histonet Sent: Wed, Oct 3, 2018 7:58 pm Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we

[Histonet] Paraffin Dispenser

2018-09-06 Thread Laurie Colbert via Histonet
I'm looking for a 2.5 gallon (approx) paraffin dispenser ASAP - new or refurbished.  I need to find one that is in stock and can be shipped immediately.  Vendors are welcome to respond. Thank you, Laurie Redmond Path MD  Los Angeles, CA ___ Histonet ma

Re: [Histonet] Giemsa vs Diff Quik

2018-09-06 Thread Laurie Colbert via Histonet
We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric bx's.  I believe you can use the Diff Quik for FNA's, especially if it is just for a preliminary diagnosis and you will be staining with H&E or a cytology stain later.  We get our Diff Quik from Medical Chemical Corporati

[Histonet] Per diem Histotech position at Path MD

2018-08-27 Thread Laurie Colbert via Histonet
Path MD is looking for a per diem histotech to work Saturdays and as needed during the week.  Must be ASCP registered and be able to start between midnight and 2:00 am and also be able to work during the holidays. Path MD is a state of the art Pathology Lab located in West Hollywood.  We are lo

[Histonet] Special stains and hoods

2018-08-21 Thread Laurie Colbert via Histonet
I would like to know how many people perform (manual) routine special stains under a hood.  We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - but we do them on the counter, not under a hood.  We have been told the staining needs to be done under a hood, but I have never done that in

Re: [Histonet] Microtomes

2018-07-18 Thread Laurie Colbert via Histonet
We have three Thermo 355S's.  They are nice because you can use them in the automated mode or manual mode.  All of our HT's use the manual mode, so now we just buy the manual version. Laurie Redmond HT (ASCP) Path MD -Original Message- From: Piche, Jessica via Histonet To: histonet S

[Histonet] Excelsior Tissue Processor

2018-07-12 Thread Laurie Colbert via Histonet
If there is anyone out there who is using the Thermo Fisher Excelsior Tissue processor for biopsies, would you please contact me directly at my personal email?  I have a question about your processing program. Thanks, Laurie Redmond ___ Histonet mailin

Re: [Histonet] pH of formalin

2018-06-06 Thread Laurie Colbert via Histonet
We monitor the concentration and pH of our recycled formalin weekly, but since it has been consistently within range I may start monitoring monthly. -Original Message- From: Baldwin, Kathy via Histonet To: 'histonet@lists.utsouthwestern.edu' Sent: Wed, Jun 6, 2018 11:25 am Subject: [Hi

[Histonet] Silver disposal

2018-06-06 Thread Laurie Colbert via Histonet
I'm wondering how others dispose of their silver waste from a GMS stain?  We have always collected and disposed of it separately from our other chemical waste.  We have a 55 gallon drum where we dispose of formalin, alcohol and xylene, and I was told by several people that we could just add the

[Histonet] V600E

2018-06-06 Thread Laurie Colbert via Histonet
Can anyone tell me about the V600E antibody?  Our pathologist wants to add it to our menu of IHC stains, and I've never heard of it.  Sources? Thanks, Laurie Redmond ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern

[Histonet] p16 Antibody

2018-05-17 Thread Laurie Colbert via Histonet
We have been using Ventana's p16 antibody and detection kit on an IHC stainer that is not Ventana's stainer.  We have been told that these reagents are now obsolete, but that they can be purchased in dispensers which are customized for Ventana's own stainer.  Can I simply just dispense their rea

[Histonet] Special Stain Billing

2018-04-24 Thread Laurie Colbert via Histonet
Is a retic stain a Group 1 stain or a Group 2 stain?  I thought Group 1 was for micro-organisms and silver stains, but I just read that a retic may be a Group 2 stain. Laurie Redmond ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://li

[Histonet] Histotech positions available in Los Angeles

2018-04-12 Thread Laurie Colbert via Histonet
Path MD, a state of the art pathology lab located in Los Angeles/West Hollywood, has 3 histotechnician positions available immediately.  Path MD offers full medical, dental and vision insurance and also offers a matching 401(k) plan.  We are looking for reliable and self-motivated histotechs to

Re: [Histonet] Slide labels falling off- Slidemate AS

2018-03-28 Thread Laurie Colbert via Histonet
Stefanie, When you say "labels" are  you talking about paper labels or the print on the slides from the Slidemate?? I will assume that you mean the print, and yes, I have had the problem in the past where the print came off during staining and/or you could literally wipe the print off with a k

[Histonet] Supervisor and per diem histotech positions available in Los Angeles

2018-03-28 Thread Laurie Colbert via Histonet
Path MD, a state of the art private pathology lab located in West Hollywood, CA, is looking for a full time Histology supervisor to join our team.  The position is available immediately, Monday-Friday, 4:00 am to 12:30 pm.  HT(ASCP) and bachelors degree required, and HTL(ASCP) preferred.  At lea

[Histonet] Per diem histotech needed for nights, Los Angeles area

2018-03-24 Thread Laurie Colbert via Histonet
PATH MD is looking for a motivated and reliable per diem histotech to work occasional nights starting at 9:00 pm.  The job would consist mainly of embedding and some cutting, but knowledge in all aspects of histology preferred.  Must be ASCP certified and have at least one year of histology exp

[Histonet] Supervisor Position in Los Angeles

2018-01-28 Thread Laurie Colbert via Histonet
Immediate full time opening for Histology Supervisor at PATH MD Lab in West Hollywood, CA.  Monday-Friday, 4:00 am to 1:00 pm, no weekends;  Must be ASCP certified and have at least 5 years supervisory experience; HTL(ASCP) preferred, but will consider HT(ASCP), and Bachelors degree also preferr

[Histonet] Part time IHC position in LA area

2017-11-10 Thread Laurie Colbert via Histonet
I am posting this for a friend of mine who was recently laid off.  He is a PhD, not a histotech, and has worked doing IHC for over 30 years.  He is very experienced and competent.  He is looking for a part time IHC position in the LA area. Please see all of his contact info below. Sabir Husain

[Histonet] Histology Supervisor Position in Los Angeles CA

2017-07-15 Thread Laurie Colbert via Histonet
PATH MD has an immediate opening for a Histology Supervisor. PATH MD is a state of the art histology lab in West Hollywood and offers competitive salaries, full health insurance and a matching 401(k) plan.  Candidates must have prior experience and be ASCP registered.  Contact David Costanzo at

Re: [Histonet] Protocol?

2015-07-07 Thread Laurie Colbert
Nine slides with 3 levels each, trichrome and elastic stain. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential

[Histonet] Thermo Excelsior Processor

2015-06-18 Thread Laurie Colbert
directly to discuss this. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information

[Histonet] Histotech positions in Los Angeles

2015-06-16 Thread Laurie Colbert
stains) required, but the main duty would be embedding. Both positions are available immediately. We are a growing lab and have a great "team" environment. Please forward all resumes to this email. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles,

[Histonet] Over- decalcified tissue

2015-04-29 Thread Laurie Colbert
I have tissue that was left in decal over the weekend and now has very poor nuclear staining. Is there a "fix" for this so that I can get better nuclear staining (other than restaining for a long time in the hematoxylin)? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Be

[Histonet] RE: Cutting of Paraffin Blocks

2015-03-25 Thread Laurie Colbert
Heck no, crack that whip! Give them practice tissue that isn't important so they won't be afraid of ruining something. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf

RE: [Histonet] Masson trichrome and H and E

2015-03-03 Thread Laurie Colbert
Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around $60.00. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Tuesday, March 03, 2015

[Histonet] Full Time HT position in Los Angeles, CA

2015-01-06 Thread Laurie Colbert
. Proficiency in embedding, cutting and special stains is required. PATH MD offers full medical, dental, and vision benefits as well as a 401(k) plan. Interested applicants may send their resumes to: lcolb...@pathmdlabs.com Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los

[Histonet] Prostate Needle Bx

2014-12-03 Thread Laurie Colbert
How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx&

[Histonet] RE: back orders from various vendors

2014-12-02 Thread Laurie Colbert
Yes, I am having this issue with one vendor in particular (which I won't mention). It has affected our operations and it is very frustrating! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] RE: Water baths

2014-12-02 Thread Laurie Colbert
Yes, I have always done that at any lab I worked at. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information

[Histonet] HPV ISH

2014-11-20 Thread Laurie Colbert
What do others do for HPV ISH? Do you do the stain yourself in-house, or do you send it out? I know there used to be a stainer specifically for ISH - does this still exist and who is the vendor? I heard there is some kind of conflict between vendors and the FDA?? Laurie Colbert, HT (ASCP

[Histonet] Storage of Glacial Acetic Acid

2014-10-16 Thread Laurie Colbert
How do you store GAA? As I recall, it should not be stored with other acids in an acid cabinet. I'm think it should be stored with flammables?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] Labeling Errors

2014-10-14 Thread Laurie Colbert
Is there a national average or benchmark for "acceptable" labeling errors in Histology? Laurie Colbert HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] slide id

2014-10-03 Thread Laurie Colbert
We do. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by

[Histonet] GHS Labeling

2014-08-26 Thread Laurie Colbert
Does anyone know of a source that sells a "poster" that can be hung on the wall that shows all of the pictograms for the new Globally Harmonized System (GHS) labeling system? I would like a "master" list that can be referred to. Laurie Colbert, HT (ASCP) Histology Sup

[Histonet] Glassware Cleaning again

2014-08-15 Thread Laurie Colbert
Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323

RE: [Histonet] Water/Bubbles

2014-08-07 Thread Laurie Colbert
alcohol. Also, avoid shaking the excess xylene off of the rack when you take it off the stainer before coverslipping. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this

[Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770

2014-08-05 Thread Laurie Colbert
Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. Laurie Colbert, HT (ASCP) Histology Supervisor PA

[Histonet] Slides for Thermo Slidemate

2014-06-23 Thread Laurie Colbert
What slides are others using (other than Thermo's Superfrost Plus slides) that won't jam in the Slidemate and that will produce print that does not smear? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245

RE: [Histonet] Edit protocol for Shandon Gemini Varistainer

2014-05-07 Thread Laurie Colbert
Call Thermo and talk to Sherry Anderson at x562. She is awesome! She just walked me through some programming issues this morning. Thermo (800) 522-7270 x562 Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284

[Histonet] CAP Question, New Reagent Lot Confirmation of Acceptability

2014-03-26 Thread Laurie Colbert
athologist has to perform the comparison. What are other labs doing? Are the histotechs signing off on new lots? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this tr

[Histonet] RE: Cornflaking artifact

2014-03-13 Thread Laurie Colbert
mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: his

[Histonet] IHC Validation

2014-03-11 Thread Laurie Colbert
If I have validated an antibody at a specific incubation time, and then later want to decrease the incubation time, do I have to re-validate?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The

[Histonet] RE: Fungus Controls

2014-02-24 Thread Laurie Colbert
I am in need of fungus controls, if there are still some out there. I can provide shipping costs. Thanks! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this

[Histonet] Herpes I and II

2014-02-13 Thread Laurie Colbert
Can anyone tell me where I can purchase the Herpes I and II antibody combined and also IHC control slides for both type I and II - on one slide. Vendors welcome to respond. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct

[Histonet] Billing for ASR's and RUO's

2014-02-07 Thread Laurie Colbert
Do hospitals/labs get reimbursed for staining with ASR and RUO antibodies since they are not FDA-approved? What if they are validated in your own lab? Laurie Colbert, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Respirator Guidelines

2014-02-05 Thread Laurie Colbert
Are there any CAP requirements or checklist questions (I know there are OSHA requirements) that pertain to the use of respirators? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information

[Histonet] RE: Fungus contamination

2014-02-04 Thread Laurie Colbert
I have had fungal contamination in the Fast Green counterstain from Thermo. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and

[Histonet] Buffer pH for IHC

2014-01-22 Thread Laurie Colbert
What does everyone use to test the pH of their buffers for IHC? Are pH strips adequate or do you have to have an actual pH meter? If you use a meter, any suggestions on a make and model? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648

[Histonet] HistoWrap Filter Paper

2013-12-20 Thread Laurie Colbert
small specimens/curettings. The wraps can be folded to fit inside of a plastic funnel and specimens can be filtered directly into the paper. If there is similar alternative that is available, I would be interested in hearing what that is. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD

[Histonet] Nails for fungal stain

2013-12-13 Thread Laurie Colbert
My question: Would the potassium hydroxide have any bearing on the results of the fungal PAS since the nails are not fixed in formalin before going into the potassium hydroxide? Laurie Colbert, HT (ASCP) ___ Histonet mailing list Hist

[Histonet] RE: Stainer/coverslipper question

2013-12-03 Thread Laurie Colbert
extremely fast and very trouble-free. I have heard that Sakura's glass coverslipper is good. We currently use the ClearVue glass coverslipper from Thermo, and it works well. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet

[Histonet] RE: Amended Reports

2013-12-03 Thread Laurie Colbert
The specimen container and requisition should always be compared before accessioning. If the name on the specimen container label and the name on the requisition do not match, we do not accept the specimen. It should be sent back to be properly labeled. Laurie Colbert, HT (ASCP

RE: [Histonet] Problems with H&E Bleeding

2013-11-04 Thread Laurie Colbert
You probably have water and/or alcohol contamination in your xylenes. Make sure your dehydrating alcohols after the eosin are really clean. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] slide drying oven

2013-10-23 Thread Laurie Colbert
Can anyone recommend a small, forced air slide drying oven? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet

[Histonet] RE: Endometrial biopsies

2013-10-22 Thread Laurie Colbert
doesn't always help. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, October 22, 2013 4:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endome

[Histonet] Punch Biopsies

2013-09-17 Thread Laurie Colbert
essed well - they are hard to section and we get incomplete sections. For the most part, all of the other tissue is fine to cut. Is there a trick to cutting/processing punches? We have never had so much trouble with them! Laurie Colbert, HT (ASCP) Histology Supervisor PA

RE: [Histonet] Nail specimens

2013-09-17 Thread Laurie Colbert
We purchase a solution called "Nail Prep" from Stat Lab. It is wonderful!! We had a horrible time with nails before trying this. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab -Origin

[Histonet] Validation Sign off

2013-09-11 Thread Laurie Colbert
When validating IHC antibodies and detection kits (either initially or with new lots), does a pathologist have to sign off on the validation , or can it be his/her designee (such as a PhD) that signs off? The pathologist will be signing the IHC procedures. Laurie Colbert, HT (ASCP

RE: [Histonet] GI biopsy slides

2013-09-10 Thread Laurie Colbert
We cut exactly as Sheila does. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Tuesday, September 10, 2013 8:59 AM To: Elizabeth Chatfield; histonet

[Histonet] Razor Blade Holder

2013-09-03 Thread Laurie Colbert
Does anyone know where I can purchase the little plastic holder for razor blades for trimming your blocks and what the thing is called?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] IHC using Thermo antibodies and stainer

2013-08-07 Thread Laurie Colbert
If there is anyone out there who is using Thermo's Autostainer and also using their antibodies, would you mind contacting me offline? We are just starting up IHC's, and I would appreciate some feedback and information on a few issues I am having. Thanks. Laurie Colbert, HT (ASCP)

[Histonet] Recycled Xylene

2013-07-29 Thread Laurie Colbert
Does anyone out there have any problems with using recycled xylene to clean the tissue processor? I was just told that we should not use recycled xylene to clean the tissue processor. How about using recycled xylene for either processing or staining? Laurie Colbert, HT (ASCP) Histology

[Histonet] p16 control tissue

2013-07-09 Thread Laurie Colbert
We are going to be running the p16 antibody from Biocare (RUO) on skin specimens. What type of tissue should I run for a positive control? The data sheet says normal testis.. Laurie Colbert ___ Histonet mailing list Histonet

[Histonet] Full Time Openings for Histotech and Grossing Tech

2013-05-10 Thread Laurie Colbert
benefits are offered. If interested, please forward your resume to: lcolb...@pathmdlabs.com<mailto:lcolb...@pathmdlabs.com> Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 ma

[Histonet] Adenovirus controls

2013-05-06 Thread Laurie Colbert
Does anyone have a source for purchasing adenovirus control slides? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet

[Histonet] Per Diem Histotech and Part Time Grossing Tech Positions Available in Los Angeles, CA

2013-04-26 Thread Laurie Colbert
ition turning into full time in the future. The hours would be ~3 pm - 7:00 pm. Experience in grossing most types of tissue (no colon or large breast resections) is required. Please contact me at the direct line below and/or forward your resume to me if interested. Laurie Colbert, HT (ASCP) Hist

RE: [Histonet] Nail treatment and bone marrow decalcification info needed

2013-04-08 Thread Laurie Colbert
We purchase a solution called Nail Prep from Stat Lab for our nails. It works wonders! You place the nails in the solution for about 1 hour, rinse, and process as usual. Laurie Colbert, HT (ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet

[Histonet] Control Tissue

2013-03-26 Thread Laurie Colbert
Does anyone have a good source for obtaining fresh tissue (formalin fixed) for IHC validation? I would need normal and tumor of various types. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] IHC validation

2013-03-15 Thread Laurie Colbert
If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays? Laurie Colbert, HT

[Histonet] RE: QC Slide retention

2013-03-05 Thread Laurie Colbert
We keep ours long enough to fill up a 100-slide box and then dump the oldest ones. We do have a separate chart where we document daily that the H&E slide is checked, and this is kept for at least two years along with our other QC (temps, processor rotation, etc) charts. Laurie Col

[Histonet] Sponges and Processing

2013-03-04 Thread Laurie Colbert
If there is anyone out there that uses the blue sponges for biopsy specimens, would you please contact me offline? I would like to know what your processing times are. Thanks! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214

[Histonet] STP420 Tissue Processor

2013-02-12 Thread Laurie Colbert
Is there anyone out there who processes both large and small tissue on the Thermo STP420 tissue processor using both the rotation chamber and the biopsy chamber? Would you please email me directly? Thanks. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles

[Histonet] Robin Simpkins

2013-01-28 Thread Laurie Colbert
Does anyone have the phone number and/or email for Robin Simpkins at Biocare? Thanks, Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Recycled Xylene on Tissue Processor

2013-01-23 Thread Laurie Colbert
Has anyone that uses recycled xylene on the tissue processor ever noticed that it dries out the biopsies (specifically GI bx's)?? We are having issues with the GI's being dried out, and I'm wondering if this may be the cause.

[Histonet] Histotech Position for Private Lab in West Hollywood, CA

2013-01-14 Thread Laurie Colbert
preferred. Please submit resumes to: lcolb...@pathmdlabs.com<mailto:lcolb...@pathmdlabs.com> Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet m

[Histonet] Microtome blades

2013-01-09 Thread Laurie Colbert
Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab

[Histonet] RE: Re: [Histology] Congo Red Stain

2012-11-08 Thread Laurie Colbert
I've tried kits from both Richard Allan/Thermo and American Master Tech. Both staining methods are similar and very easy to perform - 20 minutes in Congo Red and counterstain in Hematoxylin. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048

[Histonet] Nails

2012-10-29 Thread Laurie Colbert
Is there anyone out there who is an "expert" on nails - specifically how to get them to stay on the slides?? We use Nair to soften the nails, and we have used plain "plus" slides, chrome alum, Elmer's glue, and gelatin - all with very limited success. Laurie Col

[Histonet] Supervisor Position Available - Pasadena, CA

2011-11-08 Thread Laurie Colbert
ition should be posted tomorrow. Please apply online at: www.huntingtonhospital.com. Feel free to email me if you would like additional information. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwester

RE: [Histonet] OK, I give up

2011-09-28 Thread Laurie Colbert
I've ordered it from Cardinal and Fisher and it comes in plastic bottles. Laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, September 28, 2011 7:49 AM To: Histonet@lists

RE: [Histonet] KP Markers

2011-09-27 Thread Laurie Colbert
I received the same info. If someone has another source, please let us all know. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Tuesday, September 27, 2011 2:18 PM To: histonet@lists.

RE: [Histonet] Audible alarm

2011-09-22 Thread Laurie Colbert
e had several occasions where a bottle was not pushed in all the way, and I can walk someone through that over the phone. Laurie colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thu

RE: [Histonet] Lysol IC

2011-09-15 Thread Laurie Colbert
I just received some Lysol IC from Lab Safety Supply. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Thursday, September 15, 2011 7:11 AM To: histonet@lists.utsouthwestern.edu S

RE: [Histonet] Bouin's without the picric acid?

2011-09-14 Thread Laurie Colbert
Tyrone, American Master Tech carries a product called Bouins 2000 which contains no picric acid. Search it on their website - they carry it in various sizes.' Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet

[Histonet] Special Stain / IHC billing - per specimen vs per block

2011-09-13 Thread Laurie Colbert
ld like to see the exact source so that we can read the full text. Does anyone know where we can find the source? Joyce Weems - you may have further info. Can you help me?? Thanks, Laurie Colbert __

[Histonet] Floater Problem

2011-09-09 Thread Laurie Colbert
they clean their forceps and their work surfaces diligently. My problem is in determining where the floater actually came from. Any suggestions?? Thanks, Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

RE: [Histonet] AMPHYL

2011-08-25 Thread Laurie Colbert
We get ours from Lab Safety Supply. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz Sent: Thursday, August 25, 2011 2:54 PM To: 'Sebree Linda A'; barbara.cr...@lpnt.net; histonet@lists.utsout

RE: [Histonet] RE: New CAP question

2011-08-25 Thread Laurie Colbert
last year), and the process was very extensive and time-consuming - but I have lots and lots of documentation to show the inspectors. Laurie -Original Message- From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org] Sent: Thursday, August 25, 2011 10:05 AM To: Laurie Colbert; Vickroy, Jim; M

RE: [Histonet] RE: New CAP question

2011-08-25 Thread Laurie Colbert
I think it may depend on the inspector. We had something similar happen in Cytology during inspection. They had no validation records for their Thin Prep processing, which they had been doing for years. They were required to validate and provide documentation to CAP. Laurie Colbert

[Histonet] Printmate ribbon

2011-08-19 Thread Laurie Colbert
For those of you who have the Thermo Fisher Printmate Cassette Labeler: I just had service in and they changed the ribbon while they were here, and the barcodes started printing really light. The tech called in to Fisher and was told that there is a bad lot of ribbons. The lot # is 6325. Fis

RE: [Histonet] Ordering special stain kits and control slides

2011-08-17 Thread Laurie Colbert
veral occasions. I'm not sure what control slides they carry, if any. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Wednesday, August 17, 2011 7:16 AM To: Histonet@

[Histonet] Cytochemistry staining - reference lab

2011-08-15 Thread Laurie Colbert
I'm looking for a reference lab in the Los Angeles area that does cytochemistry stains - specifically a TRAP stain. Does anyone know where I could send tissue sections for this stain? Laurie Colbert (626) 397-8620 ___ Histonet mailing

[Histonet] Cassette Labeler

2011-08-15 Thread Laurie Colbert
enough for our pathologists to use. Any suggestions? Vendors welcome to contact me offline. Thanks, Laurie Colbert Huntington Hospital Pasadena, CA 91105 (626) 397-8620 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

RE: [Histonet] re-cycled xylene in tissue processor

2011-08-08 Thread Laurie Colbert
We use recycled xylene for the cleaning cycle on our VIP 5's and have had no problems. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gaiser, Marcia Sent: Monday, August 08, 2011 7:34 AM To: histonet@lists.

RE: [Histonet] Where Can I Buy Pneumo Control Slides

2011-07-25 Thread Laurie Colbert
American Master Tech. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Monday, July 25, 2011 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where Can I B

[Histonet] SlideMate Procedure

2011-07-13 Thread Laurie Colbert
Does anyone have a PSLIM/SlideMate procedure that they would be willing to share with me? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Stripped H&E's - HELP!

2011-07-07 Thread Laurie Colbert
Make sure the level of the alcohols after the eosin covers the tops of the slides. It's most likely water dripping down the slides, and this cause the eosin on the sections to bleed so they looked striped. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwester

RE: [Histonet] Processing

2011-07-06 Thread Laurie Colbert
We heat from 38-40 degrees for all reagents except the paraffin, which is heated to 60 degrees. I don't use any heat at all on my small biopsies. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.ed

RE: [Histonet] Recycled Xylene

2011-05-25 Thread Laurie Colbert
We process with recycled xylene and have no problems. We also use it for staining but use fresh for coverslipping. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent

RE: [Histonet] RE: Ventana E-cadherin problem

2011-05-18 Thread Laurie Colbert
t's better. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, May 18, 2011 7:24 AM To: Walter Benton; Dessoye, Michael J; histonet@lists.utsouthwestern.

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