You should put the AFB control on a separate slide to prevent
cross-contamination.
Laurie Redmond
-Original Message-
From: ADESUPO ADESUYI via Histonet
To: histonet
Sent: Wed, Oct 3, 2018 7:58 pm
Subject: [Histonet] AFB STAIN
Hello,
I have a question please. For the AFB Stain, do we
I'm looking for a 2.5 gallon (approx) paraffin dispenser ASAP - new or
refurbished. I need to find one that is in stock and can be shipped
immediately. Vendors are welcome to respond.
Thank you,
Laurie Redmond
Path MD
Los Angeles, CA
___
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We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric
bx's. I believe you can use the Diff Quik for FNA's, especially if it is just
for a preliminary diagnosis and you will be staining with H&E or a cytology
stain later. We get our Diff Quik from Medical Chemical Corporati
Path MD is looking for a per diem histotech to work Saturdays and as needed
during the week. Must be ASCP registered and be able to start between midnight
and 2:00 am and also be able to work during the holidays.
Path MD is a state of the art Pathology Lab located in West Hollywood. We are
lo
I would like to know how many people perform (manual) routine special stains
under a hood. We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci -
but we do them on the counter, not under a hood. We have been told the
staining needs to be done under a hood, but I have never done that in
We have three Thermo 355S's. They are nice because you can use them in the
automated mode or manual mode. All of our HT's use the manual mode, so now we
just buy the manual version.
Laurie Redmond HT (ASCP)
Path MD
-Original Message-
From: Piche, Jessica via Histonet
To: histonet
S
If there is anyone out there who is using the Thermo Fisher Excelsior Tissue
processor for biopsies, would you please contact me directly at my personal
email? I have a question about your processing program.
Thanks,
Laurie Redmond
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We monitor the concentration and pH of our recycled formalin weekly, but since
it has been consistently within range I may start monitoring monthly.
-Original Message-
From: Baldwin, Kathy via Histonet
To: 'histonet@lists.utsouthwestern.edu'
Sent: Wed, Jun 6, 2018 11:25 am
Subject: [Hi
I'm wondering how others dispose of their silver waste from a GMS stain? We
have always collected and disposed of it separately from our other chemical
waste. We have a 55 gallon drum where we dispose of formalin, alcohol and
xylene, and I was told by several people that we could just add the
Can anyone tell me about the V600E antibody? Our pathologist wants to add it
to our menu of IHC stains, and I've never heard of it. Sources?
Thanks,
Laurie Redmond
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We have been using Ventana's p16 antibody and detection kit on an IHC stainer
that is not Ventana's stainer. We have been told that these reagents are now
obsolete, but that they can be purchased in dispensers which are customized for
Ventana's own stainer. Can I simply just dispense their rea
Is a retic stain a Group 1 stain or a Group 2 stain? I thought Group 1 was for
micro-organisms and silver stains, but I just read that a retic may be a Group
2 stain.
Laurie Redmond
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Path MD, a state of the art pathology lab located in Los Angeles/West
Hollywood, has 3 histotechnician positions available immediately. Path MD
offers full medical, dental and vision insurance and also offers a matching
401(k) plan. We are looking for reliable and self-motivated histotechs to
Stefanie,
When you say "labels" are you talking about paper labels or the print on the
slides from the Slidemate??
I will assume that you mean the print, and yes, I have had the problem in the
past where the print came off during staining and/or you could literally wipe
the print off with a k
Path MD, a state of the art private pathology lab located in West Hollywood,
CA, is looking for a full time Histology supervisor to join our team. The
position is available immediately, Monday-Friday, 4:00 am to 12:30 pm.
HT(ASCP) and bachelors degree required, and HTL(ASCP) preferred. At lea
PATH MD is looking for a motivated and reliable per diem histotech to work
occasional nights starting at 9:00 pm. The job would consist mainly of
embedding and some cutting, but knowledge in all aspects of histology
preferred. Must be ASCP certified and have at least one year of histology
exp
Immediate full time opening for Histology Supervisor at PATH MD Lab in West
Hollywood, CA. Monday-Friday, 4:00 am to 1:00 pm, no weekends; Must be ASCP
certified and have at least 5 years supervisory experience; HTL(ASCP)
preferred, but will consider HT(ASCP), and Bachelors degree also preferr
I am posting this for a friend of mine who was recently laid off. He is a PhD,
not a histotech, and has worked doing IHC for over 30 years. He is very
experienced and competent. He is looking for a part time IHC position in the
LA area. Please see all of his contact info below.
Sabir Husain
PATH MD has an immediate opening for a Histology Supervisor. PATH MD is a state
of the art histology lab in West Hollywood and offers competitive salaries,
full health insurance and a matching 401(k) plan. Candidates must have prior
experience and be ASCP registered. Contact David Costanzo at
Nine slides with 3 levels each, trichrome and elastic stain.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and
confidential
directly to
discuss this.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and
confidential information, including patient information
stains) required, but the main
duty would be embedding.
Both positions are available immediately. We are a growing lab and have a
great "team" environment. Please forward all resumes to this email.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles,
I have tissue that was left in decal over the weekend and now has very poor
nuclear staining. Is there a "fix" for this so that I can get better nuclear
staining (other than restaining for a long time in the hematoxylin)?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Be
Heck no, crack that whip! Give them practice tissue that isn't important so
they won't be afraid of ruining something.
Laurie Colbert, HT (ASCP)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf
Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around
$60.00.
Laurie Colbert, HT (ASCP)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Tuesday, March 03, 2015
. Proficiency in
embedding, cutting and special stains is required. PATH MD offers full
medical, dental, and vision benefits as well as a 401(k) plan.
Interested applicants may send their resumes to: lcolb...@pathmdlabs.com
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los
How do others process prostate needle bx's - what kind of cassettes do you use?
Do you use sponges? Do you wrap the tissue in lens paper? Do you process
them on a biopsy (short) run? Our bx's always seem to be less than optimal,
but we do not have problems with other small bx&
Yes, I am having this issue with one vendor in particular (which I won't
mention). It has affected our operations and it is very frustrating!
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
Yes, I have always done that at any lab I worked at.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and
confidential information
What do others do for HPV ISH? Do you do the stain yourself in-house, or do
you send it out? I know there used to be a stainer specifically for ISH - does
this still exist and who is the vendor? I heard there is some kind of
conflict between vendors and the FDA??
Laurie Colbert, HT (ASCP
How do you store GAA? As I recall, it should not be stored with other acids in
an acid cabinet. I'm think it should be stored with flammables??
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
Is there a national average or benchmark for "acceptable" labeling errors in
Histology?
Laurie Colbert HT (ASCP)
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We do.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and
confidential information, including patient information protected by
Does anyone know of a source that sells a "poster" that can be hung on the wall
that shows all of the pictograms for the new Globally Harmonized System (GHS)
labeling system? I would like a "master" list that can be referred to.
Laurie Colbert, HT (ASCP)
Histology Sup
Is bromocresol purple the same as bromcresol purple? The CAP question
regarding glassware cleaning refers to bromcresol purple, but I ordered a
powder and it is labeled as bromocresol purple.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323
alcohol. Also, avoid shaking
the excess xylene off of the rack when you take it off the stainer before
coverslipping.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this
Do others in Histology follow these CAP Guidelines for cleaning glassware?
I'm not sure if this question actually applies to Histology. I have never had
an inspector ask me about it, but I'm wondering if anyone has been questioned.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PA
What slides are others using (other than Thermo's Superfrost Plus slides) that
won't jam in the Slidemate and that will produce print that does not smear?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245
Call Thermo and talk to Sherry Anderson at x562. She is awesome! She just
walked me through some programming issues this morning.
Thermo (800) 522-7270 x562
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284
athologist has to
perform the comparison. What are other labs doing? Are the histotechs signing
off on new lots?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this tr
mountant and glass
coverslips, I'm sure you will not see the artifact.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: his
If I have validated an antibody at a specific incubation time, and then later
want to decrease the incubation time, do I have to re-validate??
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The
I am in need of fungus controls, if there are still some out there. I can
provide shipping costs. Thanks!
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this
Can anyone tell me where I can purchase the Herpes I and II antibody combined
and also IHC control slides for both type I and II - on one slide. Vendors
welcome to respond.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
Do hospitals/labs get reimbursed for staining with ASR and RUO antibodies since
they are not FDA-approved? What if they are validated in your own lab?
Laurie Colbert, HT (ASCP)
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Are there any CAP requirements or checklist questions (I know there are OSHA
requirements) that pertain to the use of respirators?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information
I have had fungal contamination in the Fast Green counterstain from Thermo.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
The information contained in this transmission may contain privileged and
What does everyone use to test the pH of their buffers for IHC? Are pH strips
adequate or do you have to have an actual pH meter? If you use a meter, any
suggestions on a make and model?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648
small
specimens/curettings. The wraps can be folded to fit inside of a plastic
funnel and specimens can be filtered directly into the paper. If there is
similar alternative that is available, I would be interested in hearing what
that is.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
My question: Would the
potassium hydroxide have any bearing on the results of the fungal PAS since the
nails are not fixed in formalin before going into the potassium hydroxide?
Laurie Colbert, HT (ASCP)
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extremely fast and very
trouble-free. I have heard that Sakura's glass coverslipper is good. We
currently use the ClearVue glass coverslipper from Thermo, and it works well.
Laurie Colbert, HT (ASCP)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet
The specimen container and requisition should always be compared before
accessioning. If the name on the specimen container label and the name on the
requisition do not match, we do not accept the specimen. It should be sent
back to be properly labeled.
Laurie Colbert, HT (ASCP
You probably have water and/or alcohol contamination in your xylenes. Make
sure your dehydrating alcohols after the eosin are really clean.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
Can anyone recommend a small, forced air slide drying oven?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
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doesn't always help.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Tuesday, October 22, 2013 4:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Endome
essed well - they are
hard to section and we get incomplete sections. For the most part, all of the
other tissue is fine to cut. Is there a trick to cutting/processing punches?
We have never had so much trouble with them!
Laurie Colbert, HT (ASCP)
Histology Supervisor
PA
We purchase a solution called "Nail Prep" from Stat Lab. It is wonderful!! We
had a horrible time with nails before trying this.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
-Origin
When validating IHC antibodies and detection kits (either initially or with new
lots), does a pathologist have to sign off on the validation , or can it be
his/her designee (such as a PhD) that signs off? The pathologist will be
signing the IHC procedures.
Laurie Colbert, HT (ASCP
We cut exactly as Sheila does.
Laurie Colbert, HT (ASCP)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Tuesday, September 10, 2013 8:59 AM
To: Elizabeth Chatfield; histonet
Does anyone know where I can purchase the little plastic holder for razor
blades for trimming your blocks and what the thing is called??
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
If there is anyone out there who is using Thermo's Autostainer and also using
their antibodies, would you mind contacting me offline? We are just starting
up IHC's, and I would appreciate some feedback and information on a few issues
I am having. Thanks.
Laurie Colbert, HT (ASCP)
Does anyone out there have any problems with using recycled xylene to clean
the tissue processor? I was just told that we should not use recycled xylene
to clean the tissue processor. How about using recycled xylene for either
processing or staining?
Laurie Colbert, HT (ASCP)
Histology
We are going to be running the p16 antibody from Biocare (RUO) on skin
specimens. What type of tissue should I run for a positive control? The data
sheet says normal testis..
Laurie Colbert
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benefits
are offered. If interested, please forward your resume to:
lcolb...@pathmdlabs.com<mailto:lcolb...@pathmdlabs.com>
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 ma
Does anyone have a source for purchasing adenovirus control slides?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
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ition
turning into full time in the future. The hours would be ~3 pm - 7:00 pm.
Experience in grossing most types of tissue (no colon or large breast
resections) is required.
Please contact me at the direct line below and/or forward your resume to me if
interested.
Laurie Colbert, HT (ASCP)
Hist
We purchase a solution called Nail Prep from Stat Lab for our nails. It works
wonders! You place the nails in the solution for about 1 hour, rinse, and
process as usual.
Laurie Colbert, HT (ASCP)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet
Does anyone have a good source for obtaining fresh tissue (formalin fixed) for
IHC validation? I would need normal and tumor of various types.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
If a lab is just starting up IHC for the first time and has to validate both
the IHC stainer and the AB's/detection kit, does the validation have to be done
using actual patient cases - or can you use strictly control tissue and
purchased microarrays?
Laurie Colbert, HT
We keep ours long enough to fill up a 100-slide box and then dump the oldest
ones. We do have a separate chart where we document daily that the H&E slide
is checked, and this is kept for at least two years along with our other QC
(temps, processor rotation, etc) charts.
Laurie Col
If there is anyone out there that uses the blue sponges for biopsy specimens,
would you please contact me offline? I would like to know what your processing
times are. Thanks!
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214
Is there anyone out there who processes both large and small tissue on the
Thermo STP420 tissue processor using both the rotation chamber and the biopsy
chamber? Would you please email me directly? Thanks.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles
Does anyone have the phone number and/or email for Robin Simpkins at Biocare?
Thanks,
Laurie Colbert
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Has anyone that uses recycled xylene on the tissue processor ever noticed that
it dries out the biopsies (specifically GI bx's)?? We are having issues with
the GI's being dried out, and I'm wondering if this may be the cause.
preferred. Please submit resumes
to: lcolb...@pathmdlabs.com<mailto:lcolb...@pathmdlabs.com>
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
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Histonet m
Does anyone know who I can purchase Accu-edge blades from, other than Cardinal
or American Master Tech?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
I've tried kits from both Richard Allan/Thermo and American Master Tech. Both
staining methods are similar and very easy to perform - 20 minutes in Congo Red
and counterstain in Hematoxylin.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
Is there anyone out there who is an "expert" on nails - specifically how to get
them to stay on the slides?? We use Nair to soften the nails, and we have used
plain "plus" slides, chrome alum, Elmer's glue, and gelatin - all with very
limited success.
Laurie Col
ition should be posted tomorrow. Please apply online at:
www.huntingtonhospital.com. Feel free to email me if you would like
additional information.
Laurie Colbert
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I've ordered it from Cardinal and Fisher and it comes in plastic
bottles.
Laurie
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Wednesday, September 28, 2011 7:49 AM
To: Histonet@lists
I received the same info. If someone has another source, please let us
all know.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M.
Gorham
Sent: Tuesday, September 27, 2011 2:18 PM
To: histonet@lists.
e had several occasions where a bottle was not pushed in all the
way, and I can walk someone through that over the phone.
Laurie colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Thu
I just received some Lysol IC from Lab Safety Supply.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
barbara.cr...@lpnt.net
Sent: Thursday, September 15, 2011 7:11 AM
To: histonet@lists.utsouthwestern.edu
S
Tyrone,
American Master Tech carries a product called Bouins 2000 which contains
no picric acid. Search it on their website - they carry it in various
sizes.'
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet
ld like to see the exact source so that we can read the full text.
Does anyone know where we can find the source?
Joyce Weems - you may have further info. Can you help me??
Thanks,
Laurie Colbert
__
they clean their forceps and their work surfaces
diligently. My problem is in determining where the floater actually
came from. Any suggestions??
Thanks,
Laurie Colbert
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We get ours from Lab Safety Supply.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae
Staskiewicz
Sent: Thursday, August 25, 2011 2:54 PM
To: 'Sebree Linda A'; barbara.cr...@lpnt.net;
histonet@lists.utsout
last year), and the
process was very extensive and time-consuming - but I have lots and lots
of documentation to show the inspectors.
Laurie
-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org]
Sent: Thursday, August 25, 2011 10:05 AM
To: Laurie Colbert; Vickroy, Jim; M
I think it may depend on the inspector. We had something similar happen
in Cytology during inspection. They had no validation records for their
Thin Prep processing, which they had been doing for years. They were
required to validate and provide documentation to CAP.
Laurie Colbert
For those of you who have the Thermo Fisher Printmate Cassette Labeler:
I just had service in and they changed the ribbon while they were here,
and the barcodes started printing really light. The tech called in to
Fisher and was told that there is a bad lot of ribbons. The lot # is
6325. Fis
veral occasions. I'm
not sure what control slides they carry, if any.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Wednesday, August 17, 2011 7:16 AM
To: Histonet@
I'm looking for a reference lab in the Los Angeles area that does
cytochemistry stains - specifically a TRAP stain. Does anyone know
where I could send tissue sections for this stain?
Laurie Colbert
(626) 397-8620
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enough for our pathologists to use. Any suggestions?
Vendors welcome to contact me offline.
Thanks,
Laurie Colbert
Huntington Hospital
Pasadena, CA 91105
(626) 397-8620
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We use recycled xylene for the cleaning cycle on our VIP 5's and have
had no problems.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gaiser,
Marcia
Sent: Monday, August 08, 2011 7:34 AM
To: histonet@lists.
American Master Tech.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
wanda.sm...@hcahealthcare.com
Sent: Monday, July 25, 2011 1:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Where Can I B
Does anyone have a PSLIM/SlideMate procedure that they would be willing
to share with me?
Laurie Colbert
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Make sure the level of the alcohols after the eosin covers the tops of
the slides. It's most likely water dripping down the slides, and this
cause the eosin on the sections to bleed so they looked striped.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwester
We heat from 38-40 degrees for all reagents except the paraffin, which
is heated to 60 degrees. I don't use any heat at all on my small
biopsies.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.ed
We process with recycled xylene and have no problems. We also use it
for staining but use fresh for coverslipping.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Marshall, Kimberly K
Sent
t's better.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla,
Melissa
Sent: Wednesday, May 18, 2011 7:24 AM
To: Walter Benton; Dessoye, Michael J; histonet@lists.utsouthwestern.
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