YJ,
You would probably be interested in this:
Scale: a chemical approach for fluorescence imaging and reconstruction
of transparent mouse brain. Hiroshi Hama et al., Nature Neuroscience
14, 1481–1488 (2011)
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Message: 1
Date: Fri, 18 Nov 2011 09:17:36 -0800
From: aget liang
If your antibodies are labeled with quantum dots and you strip the
antibodies (e.g. with low pH) you will no longer have labeled targets in
your tissue. Not clear if you understood this from your post.
Message: 2
Date: Sun, 30 Oct 2011 11:58:19 -0700
From: Claire Weston
It may however if you're doing immunofluorescence, as MB has been used
to quench autofluorescence (Seeing the Wood through the Trees: A Review
of Techniques for Distinguishing Green Fluorescent Protein from
Endogenous Autofluorescence; Nicholas Billinton and Andrew W. Knight,
Analytical
Kevin,
This article has a nice photo showing mouse trigeminal ganglia. Google
images is your friend--
Mike
Nature Protocols 2, - 152 - 160 (2007)
Production of dissociated sensory neuron cultures and considerations for
their use in studying neuronal function and plasticity
Sacha A Malin,
Ashley,
Phosphatases are relatively resistant to fixation which is why the
histochemical phosphatase methods work. You may want to try putting
phosphatase inhibitors in you fix or post-fix solutions to stop
dephosphorylation, and see if it makes a difference in your ihc. I
think Sigma has a
Frauke,
There are some published methods for doing Golgi on brain sections:
Freund TF, Somogyi P. The section-Golgi impregnation procedure. 1.
Description of the method and its combination with histochemistry after
intracellular iontophoresis or retrograde transport of horseradish
a motion is hereby offered to have the active members of histonet vote
this troll off the island, or at least request that the list moderator
do so. this person has proven to have absolutely nothing of value to
contribute to this group, and is only intent on disrupting it.
second?
