Hi:
Have you considered the possibility that there is something wrong with
the microtome? Sadly equipment that is "inherited" can have issues.
Perhaps a good cleaning is in order -
By the way, what the make/model of the tome? I have a couple of B&L's
that are available for a trade --;-)
Late
With phloroglucinol you are staining for lignins which can be present in
varying abouts in plant tissue. Also, please note that different types
of lignins occur in different types of plants at different levels so
sadly negative results don't mean much.
Other carbos to consider are cellulose or c
Scott:
Does the chuck have a clamp in it? If so soak a piece of soft wood that
fits in the chuck in 100% ETOH (pulling a vacuum on it would help) and
transition into paraffin. Once its soaked in paraffin pull it out and
allow to solidify. Your tissue will need to be flat embedded in a
simple bo
Hi:
I'm not sure if this helps but Botanists have long used tert-butanol
(not N-butanol) to embed tissue in paraffin. This classic protocol was
popularized by D. A. Johansen in the mid 1930's and involves transition
from water to alcohol to t-butanol (I can supply more detailed info if
you need
Hi:
There are a couple of sources besides the makes and vendors but as
always it is "buyer beware".
eBay - you can go to www.ebay.com and do a search for microtome, right
now they have several for sale. The good thing about eBay is the
shipping is often reasonable and you can get a good deal. T
