[Histonet] Okajima stain control

HI, What would I use for a control for the Okajima stain (hemoglobin). This is my first time doing this stain so any advice would help as well. Thanks! Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (f

[Histonet] Leica part, thank you

Thank you for the responses. You have given me lots of leads. Sincerely, Betsy Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.org | www.texasheart.org 6770 Bert

[Histonet] Leica 2135 parts

Hi, I am looking for a flex shaft cable for our Leica microtome 2135. I have been told by Leica they no longer service or carry parts for this machine any longer. Ours is in excellent condition and a new one is not in our budget. Is there a Leica “junkyard”? Thanks. Betsy Betsy Molinari HT(ASCP

RE: [Histonet] Karnovsky's

rjbu...@yahoo.com] Sent: Wednesday, June 19, 2013 1:21 PM To: Molinari, Betsy; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Karnovsky's Are you referring to a whole heart? If that is the case I think the fixation of the tissue will be very poor because Karnovsky's penetrate ve

[Histonet] Karnovsky's

Hi, I received a heart that was fixed in 2% Karnovsky’s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remov

[Histonet] Movats problem solved

Good Friday to all. Histology 101. The Alcian Blue dye itself had lost its efficacy. I borrowed a solution from another lab and voila, perfect staining. Thank you to everyone for their suggestions. I will certainly keep them on file in the event this ever happens again. Have a good weekend. Bets

RE: [Histonet] another Movats question (paraffin)

y 28, 2013 6:40 PM To: Molinari, Betsy Subject: Re: [Histonet] another Movats question (paraffin) Any chance this is a new batch of alcian blue? From where did you purchase it? I have a theory, but would like some more information first. Peggy A. Wenk, HTL(ASCP)SLS Lee & Peggy Wenk -

[Histonet] RE: another Movats question (paraffin)

Message- From: Donella Stillings [mailto:dstilli...@unipathdx.com] Sent: Tuesday, May 28, 2013 10:56 AM To: Molinari, Betsy Subject: RE: another Movats question (paraffin) Are you using Weigert's Hematoxylin or regular Hematoxylin? Donella -Original Message- From: histonet

[Histonet] another Movats question (paraffin)

Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then i

[Histonet] "harder" paraffin

Hi Histonetters, Good Friday to you all. Do you know if there is a “harder” paraffin that may offer more support to the tissue while cutting? I currently use Paraplast Plus and love it, but now I am cutting some polymer material and there is some tearing and was thinking that maybe a different

[Histonet] Verhoeff's with a green counterstain

Hi, I have not heard of this stain before, but saw mention of it in a paper. I could not find a protocol. Could someone tell me more about it? Thanks! Betsy Molinart HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houaton, TX 77030 832-355-6524 (lab) 832-355-6812 (fax)

[Histonet] Zinc-Tris mouse hearts

Hi, I am going to have some mouse hearts that have been fixed in zinc-Tris fixative. I have never worked with this before. My question is about processing. After doing some looking, I found an article that advised checking the solutions on the processor , the first couple of alcohols , to be su

[Histonet] calcium buildup

Hi, Does anyone know of a way to remove calcium buildup on the wash nozzles in the water reservoirs. I have the Tissue-Tek DRS 2000. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) [THI Celebr

[Histonet] FW: bone regrowth

From: Molinari, Betsy Sent: Thursday, June 28, 2012 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: bone regrowth Hi all, I have a couple of questions regarding decal and staining. I have done some searching but could use some hands on experiences. It involves bone regrowth. The sample

[Histonet] Von Kossa vs Alizarin Red

Hi, Under what circumstances would you prefer one over the other? Thanks! Happy Friday! I hope all you convention goers ( is that even a word??) are having a great time! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355

RE: [Histonet] How many tissues an histo tech is suppose to cut per

SorryI meant 300 in a 8hr day..including embedding, etc -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, June 30, 2011 6:10 AM To: histonet@lists.utsouthwestern.edu

RE: [Histonet] How many tissues an histo tech is suppose to cut per

I once went to an interview at a research lab , will remain unnamed, and they asked if I could cut 300 /hr. I said "Is this a trick question?" They just stared back. I withdrew my app. LOL Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 83

[Histonet] stainless steel cassettes

Hi , Does anyone have any stainless steel cassettes they would like to sell or does anyone know where we could purchase them? As always, thank you for your help and happy hump day! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 7703

[Histonet] trichrome/ frozen cardiac tissue

Hi, I have some frozen house hearts that were perfused with 4% paraformaldehyde. I found protocols for FF, but not fixed frozen. I tried staining them with my usual Gomori I use on my paraffin blocks but the doc thinks they picked up too much red, not enough green. Any suggerstions..I need to g

RE: [Histonet] Bielschowskys Stain

Glawon, Thank you for your help. I appreciate it. Betsy -Original Message- From: Glawon Flood [mailto:smoo...@gmail.com] Sent: Tuesday, March 15, 2011 2:33 PM To: Molinari, Betsy Subject: Re: [Histonet] Bielschowskys Stain Betsy, I don't know of any modifications for Eager's

[Histonet] Bielschowskys Stain

Hi , An investigator would like show axonal degeneration in peripheral nerves. Is Bielschowsky's the preferred stain? Thanks! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax)

[Histonet] Bielschowskys stain

Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/li

RE: [Histonet] H&E stain uneven

I had this problem awhile back. Did you change your solvent for deparaffinization? I changed from xylene to another solvent and realized I had to extend the time my slides were in it. Betsy Molinari HT(ASCP) Texas Heart Institue Cardiovascular Pathology 1101 Bates Street Houston, TX 77030 832-35

RE: [Histonet] 70% alcohol

That is how I handle ours as well. Start in 95% and proceed with the normal processing protocol. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.u

[Histonet] frozen lung sections

Hi all, I have received some lung samples on OCT. These lungs have not been in PBS or any fixative. They went directly from dissection into OCT and frozen. I cannot get a section. The temp of the cryostat is -20. I have done some reading and it seems the best results come from immersion in PBS

[Histonet] staining questions

Good morning! I will be staining vessels for proteoglycans using Alcian Blue. I was going to use the 2.5pH protocol with a Nuclear Fast Red counterstain. Does anyone have any other suggestion? The investigator does not want Movats. Also, I normally use PTAH as my fibrin stain for clots but was

[Histonet] Movats on mouse aortas

Hi, I have had a problem with "splotchy" elastin staining on certain sections of mouse aorta. I use the Modified Movats and do not use a microwave. But from this one lab some areas of the elastic fibers will retain the hematolylin after differentiation while some areas lose it completely, all in

[Histonet] Leica RM2165 instruction handbook

Hi, Does anyone have a handbook for this microtome that they would be willing to fax to me? We inherited one with no instruction book. I have searched online and cannot find it. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houst

RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT

This had nothing to do with an inspection, but follows this comment. There were 3 plants in our lab and there was one histotech who starting sneezing and got congested whenever he sat at his microtome. The plants were in front of him on a raised shelf. Finally we had a thought it may be the plan

RE: [Histonet] KP Marker Plus

Mercedes Medical 800-331-2716 Betsy Molinari HT (ASCP) Texas heart Institute Cardiovascular Pathology Houston,TX 77090 832-355-6524 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Fr

[Histonet] glass

I have some glass knife strips measuring 16"x1.5"x1/4" . We found them during some lab spring cleaning. They are free to whoever may want them. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-681

[Histonet] decolorizing MMA

Good morning, Is it possible to decolorize MMA slides? Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing l

RE: [Histonet] dumping formalin

: Friday, March 13, 2009 9:37 AM To: Molinari, Betsy Subject: RE: [Histonet] dumping formalin Betsy, Nice to hear from a (former)Bostonite. Although, if you are from Boston, you are always from Boston! You are right: the city did spend a lot of money cleaning up the harbor and all that feeds into

[Histonet] dumping formalin

We collect ours and the Hazardous Waste Dept of the hospital collects it. Now what they do with it I have no idea. We collect all our waste, fixatives, stains etc. I know of at least 2 facilities in Houston that pour theirs down the drain. I am from Boston I was so pleased to read the steps they

RE: [Histonet] aqueous mounting media

I use Aqua-Mount. I have never had a problem. It is from Lerner Laboratories but I think you can get it from any major vendor. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From:

[Histonet] contrast

Hi, I have some mouse hindlimbs that have catheterized with Omnipaque. The Omnipaque contains 302 ml of iohexol which is equivalent to 140 mg of organic iodine per ml. The mice will be sacrificed immediately after being radiographed. They would like them to be processed in paraffin. I am wonderin

RE: [Histonet] uncertified techs in Histology

Thanks for the clarification Bernice! -Original Message- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Wednesday, February 18, 2009 1:31 PM To: Molinari, Betsy Subject: RE: [Histonet] uncertified techs in Histology Betsy I was referring to those researchers that

FW: [Histonet] uncertified techs in Histology

-Original Message- From: Molinari, Betsy Sent: Wednesday, February 18, 2009 12:54 PM To: 'Bernice Frederick' Subject: RE: [Histonet] uncertified techs in Histology I don't like the generality of this statement. I work in a research lab and my "stuff" is no

RE: [Histonet] gma/h...@e

Richard, I use the same mountant that I use for my paraffin slides, Sakura Tissue Tek Glas. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, Texas 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists

RE: [Histonet] Mounting media question

I use Sakura Tissue Tek Glas mounting media. I can file the next day. Betsy Molinari HT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Wednesday, February 04, 2009 10:56 AM To: hist

RE: [Histonet] Plastic embedding

You and the rest of the world Armando. Information like this is costly and hard to come by. Best of luck none the less. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77225 832-355-6524 832-355-6812 (fax) -Original Message- From: his

RE: [Histonet] Coverslippers

I use the Sakura Tissue Tek Glas and love it. Low maintenance, easy to clean and simple to program. >From what I understand if you have a high production of slides glass may be too slow . Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Avenue MC1-283 Houston,T

RE: [Histonet] Paraffin wax

We have used Paraplast Plus for years. For both processing and embedding. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [

FW: [Histonet] honey as a formalin substitute

I had kept this in my emails. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Truscott, Tom Sent: Thursday, September 21, 2006 5:07 PM To: Rene J Buesa; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitu

RE: [Histonet] RE: Histonet Digest, Vol 60, Issue 32

Hi Rudy, I may have some. I will look around. Does it have to be fresh, unfrozen? Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Avenue Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTE

[Histonet] wavy mid myocardium

Hi, I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in 70% for anywhere from 24-48hrs. They were processed on a short run. The epicardium looks good but the mid myocardium is wavy. 15 min 80% 15 min 95 x 2 15 min 100% x 2 15 min Xylenes x2 10 min paraffin x 4 Any sugg