ProLong Gold (Invitrogen)
Montina J. Van Meter, HT (ASCP)
Lab Manager
Dept. of Autonomic Neuroscience
Pennington Biomedical Research Center
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Guiller
Carmen,
I routinely stain 40-60 micron sections for IHC. I currently run all my
IHC with a free-floating protocol which allows for maximum staining
penetration from both sides of the section and then mount them on the
slides. In the past, we mounted the 60um sections on (300 bloom)
gelatin coate
Shelly,
Is your new employee a certified histotech? Did she rotate through
clinical labs while in histo school? I would think she would have
experienced this sensitivity during that time period as well.
Tina
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:hi
Contact Leica to see if they have any demo models for sale.
Sent from my iPhone
On Aug 26, 2011, at 2:10 AM, "Jette Barlach" wrote:
> Does anyone have either a used Leica Polycut E or SM 2500 for sale.
> For the present we are using a very old (50 years) Reichert-Jung heavy duty
> microtome
ProLong Gold (Invitrogen)
Montina J. Van Meter, HT (ASCP)
Lab Manager
Dept. of Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA 70791
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsou
Susan,
I have stained 60um fixed sections on plus slides without any problem. I
free float the sections in a multi-well plate containing PBS or TBS.
When mounting the sections onto the plus slide, I wipe away the excess
fluid after EACH section, so that the previous section will not be
exposed to
National Society for Histotechnology (Sept 16-21, 2011). www.nsh.org
Montina J. Van Meter, HT (ASCP)
Lab Manager
Dept. of Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA 70791
225-763-2564
-Original Message-
From: histonet-boun...@list
Invitrogen - cat# 750118
Sent from my iPhone
On Jul 5, 2011, at 10:46 AM, "Reynolds,Donna M" wrote:
> I just learned the company making the Brigati Stable DAB is no longer making
> it. Does anyone know if someone else has been able to pick up this
> formulation and produce it or are plannin
Dear Histonet Colleagues,
The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 3 & 4, 2011, in Baton Rouge, LA.
Meeting location: The Embassy Suites Hotel
4914 Constitution Ave.
Dear Histonet Colleagues,
The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 3 & 4, 2011, in Baton Rouge, LA.
Meeting location: The Embassy Suites Hotel
4914 Constitution Ave.
Nick,
I too have tried to limit my exposure after making it from scratch for
30+ years. I currently purchase it from USB Corp. located in Cleveland
Ohio. They have been acquired by Affymetrix and you will be directed to
that website when you type in www.usbweb.com.
Cat.# 19943 1LT Paraformal
Neil,
When I read the paper in question, it reminded me of the pretreatments
one would use in an In Situ Hybridization protocol. I have used this
protocol in the past and it doesn't seem to harm the tissue as long as
the tissue has been adequately perfused. We use the pSTAT3 #9131 (Cell
Signaling
Stacy,
How are you preserving the tissue prior to sectioning (i.e. liquid nitrogen,
isopentane, etc.)? Flash freezing is essential when dealing with unfixed
tissue.
Tina
Sent from my iPhone
On Feb 13, 2011, at 3:10 AM, "Stacy Deppeler" wrote:
> Hi Histonetters,
>
> I've been trying to pe
PM
To: histonet@lists.utsouthwestern.edu
Cc: Montina Van Meter; Guillermo Palchik
Subject: Re: floating section incubation times
Hi Histonet, Tina & Gil
I work on the same material as Tina and just wanted to echo
her "overnight" comments with the addition that I routinely
incubate
Gil,
I routinely incubate free-floating 40um rat brain sections overnight or
longer @ 4 degrees centigrade according to the particular antibody that
I am working with. Our sections are perfused in 4% paraformaldehyde and
cryoprotected with 30% sucrose prior to sectioning. How long are you
fixin
Jennifer,
I don't use the Mach 1 for animal tissue. I am working with rat tissue
and use the Biocare Rabbit on Rodent or Mouse on Rat HRP or AP Polymers.
I have absolutely no background and my sections are 40um thick. Perhaps
you are using the wrong kit.
Hope this helps,
Tina
-Original Me
Hello Histonetters,
The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.
Meeting location: The Embassy Suites Hotel
414 Constitution Ave.
Hello Histonetters,
The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.
Meeting location: The Embassy Suites Hotel
414 Constitution Ave.
Hello Histonetters,
The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.
Meeting location: The Embassy Suites Hotel
414 Constitution Ave.
Hello Histonetters,
The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.
Meeting location: The Embassy Suites Hotel
414 Constitution Ave.
My high school guidance counselor received a letter from Barbara
Hrapchak, co-author of "The Theory and Practice of Histotechnology and
director of the Ohio State University Hospital School of Histotechnology
in 1975. I knew that I wanted to go into a scientific field that
involved dissection as I
Here is Ethel's contact information:
Ethel R. Macrea, HT(ASCP)QIHC
Ventana Medical Systems
HC 3 Box 1023, 22191 S. Branch Road
Tucson, AZ 85739
UNITED STATES
Phone: (520)219-8471
Fax: (502)219-8454
Email: dawnb...@earthlink.net
Montina J. Van Meter, HT (ASCP)
Lab Manager
Mike,
I free float all of my rat brain sections (40-60um) and transfer them
with a paintbrush. It takes a delicate touch to do this without causing
damage to the tissue. I do know people who use pipettes to change the
solutions. Either way works, it's a matter of personal preference.
There
I can remember back in the late 70's sitting at my microtome in a
clinical lab (that was the size of an elevator shaft = no
ventilation),where my supervisor would puff away on cigarettes with an
ashtray perched on top of her microtome. When we had inspections
everyone would put their contraband i
Yes, ThermoFisher
Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA 70791
225-763-2564
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwes
Fellow Histonetters,
The Louisiana Society for Histotechnology is pleased to announce the
26th Annual Symposium/Convention:
"Your
His
Fellow Histonetters,
The Louisiana Society for Histotechnology is pleased to announce
the 26th Annual Symposium/Convention:
"Your
Histeaux Surplus Package"
June 12 & 13, 2009
at the
B
Fellow Histonetters,
Does anyone have knowledge of guidelines or regulations on whether a
fume hood must remain "on" 24/7? In a cost cutting measure a facility
is considering turning off the fume hoods from 7pm to 5am. I have
expressed my concern with the idea because of the huge health and s
Fellow Histonetters,
The Louisiana Society for Histotechnology is pleased to announce
the 26th Annual Symposium/Convention:
"Your
Histeaux Surplus Package"
June 12 & 13, 2009
Hello Histonetters,
The Louisiana Society for Histotechnology is pleased to announce
the 26th Annual Symposium/Convention:
"Your Histeaux Surplus Package"
June
Tifei,
I routinely section rat brain at 40 microns and free-float them through the
immuno protocol. Here are a few
suggestions:
1. Did you perform a dilution series to optimize the best antibody
concentration?
2. I antigen retrieve difficult antibodies (especially with thick sections)
ProLong Gold w/ DAPI (Invitrogen/Molecular Probes)
This is a permanent mounting media - works great!
Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA 70791
225-763-2564
-Original Message-
From:
Hello Histonetters,
The Louisiana Society for Histotechnology is pleased to announce
the 26th Annual Symposium/Convention:
"Your Histeaux Surplus Package"
June 12 & 13, 2009
at the
Bourbon Orleans Hotel
717 Orleans St.
New Orleans, LA 70117
www.bourbonorleans.com
The LSH bl
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