Charles,
The easiet way to remember it is this: you want a 1:100 dilution - so you want
1 part antibody in 100 parts of diluent. I was taught that meant add 1 part
antibody to 100 parts of diluent. However, there are some who add 1 part
anitbody to 99 parts diluent.Thus you end up with 1 part
Pink and purple stainThe slide pink and not too blue.Benign, very good news
Sincerely,
Paula Sicurello
On Sat, Jul 20, 2024 at 1:29 PM, Bob Richmond via
Histonet wrote: Saffron, eosin,
Stamens of autumn crocus:
So yellow! So red!
This actually is a haiku, with the obligatory seasonal re
Jay,
Groaning and eye rolling.
Sincerely,
Paula Sicurello
On Wednesday, May 15, 2024 at 12:06:24 PM PDT, Jay Lundgren via Histonet
wrote:
The Mummy's major weakness is fire, a common weakness among the undead.
Since mummies tend to be dry and coated with various oils and resins, t
issue and ready for xylene
and wax?
Phillip Kara, HTL | Senior Research Associate
University of North Texas Health Science Center
Division of Research and Innovation
a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107
p: 918-281-9060
w:www.unthsc.edu/corelabs
From: Paula Sicurello via Histonet
Good Morning,
My question two part:
Best tissue processor ever and why?
What is percentage is your first alcohol step?
I see that lots of places start at 70% but I favor a lower %. One because
it's gentler on the tissue, and two because 10% NBF precipitates out if you
start at 70%.
Thanks for y
Good Morning and Happy Monday,
If you work at a Department of Defense (DOD) Histology lab, can you tell me
what make and model of cassette printer and slide writer you use?
Do they have an ATO?
We are in the market for both and want to know what y'all are using. Plus how
do you like whatever it i
Why use a different wax for embedding? Cost.
Those who don't fully understand the art & science of histology think a wax is
a wax is a wax.
Bon-bon,
Paula
Sent from Yahoo Mail on Android
On Sat, Feb 10, 2024 at 11:23 AM, Carl Hobbs via
Histonet wrote: Why would anyone use a
different wa
Hello Histoteckies,
What are y'all doing regarding the CAP requirement to monitor the pH of
formalin?
We buy tons and tons of the 5 gallon cubitainers and we are still debating over
how to check the pH.
Looking forward to your replies.
Toodles!
Sincerely,
Paula Sicurello
__
I'm glad to hear that the staff suggested the tracking of tasks. If I had to
guess the items causing the most trouble are:
Poorly grossed specimens if someone other than a Histotech grosses - all of
which cause poor processing: too big filling the entire cassette; too thick
squishing out the t
Is this documentation for a work/labor study to determine pricing or show upper
leadership how hands-on and time consuming histology is?
If not, then what you are asking is punitive. Not to mention that it will kill
any comradery that your histotechs may have. This will be seen as either a
com
Well..I did not pass the exam on my second attempt. Waa!
Anyway, it got me wondering: are there statistics out there that give a break
down of the pass rate of the QIHC exam?
What's the percentage of HTLs that are QIHC?
Sincerely,
Paula Sicurello
___
Hi Ken,
A few questions:
1. What is the melting point of the paraffin you use? 2. Is there a specific
reason the temperature of your water bath is 38.5 degrees? 3. Do your forceps
have any type of imperfections? Bumpy, or ridged? Something that could snag on
the sections?
If there isn't a spec
Gurtrude,
It's not a common practice anymore to use glass with a microtome. If you are
using a methacrylate you have to use glass, a razor blade isn't strong enough
to handle the shear forces.
The art of hand breaking the kniveswell that's becoming a lost art. I
can't do that anymore.
The one thing that bothers me about the preloaded programs is the paraffin
temperature is set at 65 degrees C. That's close to 10 degrees higher than the
melting point of our paraffin.
Does anyone know why the temp. is so high? To me, a temp that high will cause
problems with IHC and small bio
Hi Renee,
Brains are notorious for being difficult. Processing has to be just so,
embedding has to be just so, and sectioning? Well, just plain tricky.
It sounds like your water bath may be a smidge too cool. Brains are picky that
way. Water bath too warm? Poof! The section explodes. Too
Ted Pella, Inc. (Support small business) they sell EM, and Histology items.
Paula Sicurello
On Thu, Mar 2, 2023 at 5:19 AM, Karen Heckford CA-San Francisco via
Histonet wrote: I got mine through Thermo
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450
Good Morning Listers,
We are in the market for a new cassette printer. Has anyone used the General
Data LaserTrack FLEX system? I'm interested in how well it actually prints on
3 sides of the cassettes.
Any input about any other vendors printers is helpful. I have quotes from
General Data, Sa
Hi Brian,Unfortunately, no. The most you can do is to put it back in the oven
and let it bake forva very long time.
You won't be able to section it. It will gum up the knife edge. If they are
using a diamond knife they need to clean it right away.
To add insult to injury, the poorly polymerize
Hello Listers,
Happy Saturday, it's a beautiful day here in San Diego.
I have a question: Is there an alternative route for the experience
requirement for the QIHC? I need to get certified but I haven't been doing IHC
at my new job. I have been doing IHC since the early 1990's (remember the
V
Hello Listers,
UC San Diego Health has an opening for a part-time Histologist. HT/HTL
required. Experience in a high volume academic setting preferred. We need
someone with strong sectioning and trouble shooting skills. Familiarity with
IHC or Electron Microscopy a plus.
Go to jobs.ucsd.edu a
Hello,
We are hiring a histotechnologist for our growing histology lab. UCSD is
adding a new hospital and soon our volume will increase. We are looking for an
experienced histotech. Please go to:
https://jobs.ucsd.edu/bulletin/job.aspx?cat=health&sortby=post&jobnum_in=81586.
Sincerely,
Paula
Hi Linda,
I've set up three histology labs for animal research: one in an academic
setting, one for a pharmaceutical firm, and one for a research foundation.
1. Having money to buy the proper equipment is tricky, start with used
equipment and make due until you can afford to buy new. As long a
Hello Netters,
Does anyone have an electronic copy of the owners manual for a Tissue Tek VIP6?
If you do can you send it to me?
Sakura charges $129 for one!!
Thanks,
Paula
UC San Diego Health
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Hello Listers,
Has anyone had any problems with fixation using Richard-Allan Scientific 10%
NBF? Some of our gastric biopsies are looking a little off. The rest of the
biopsies fixed in the same NBF and processed on the same processor look fine.
This issue would have come up in the last coupl
Well Listers,
I finally get around to posting my survey. Only 35 people took it but it still
provided useful information and I got an A in my class.
Thank You to the 35 who participated
All the data: 23 HT's, 8 HTL's, 4 with other certification (2 with
HT/HTL) took my survey
Good Morning Listers,
Are there companies out there that work with traveling histologists? If so,
can you send me their contact information?
Companies dealing with traveling histologists are free to contact me as well.
Sincerely,
Paula
Paula SicurelloHistotechnology SpecialistUC San Diego Health
Good Morning Listers,
I will be closing my survey this week on Friday. If anyone else would like to
take it, please follow this link:
Histology Questionaire Survey
| |
| | | | | |
| Histology Questionaire SurveyWeb survey powered by SurveyMonkey.com. Create
your own online survey no
Good Evening Netters,
I've created a short survey using Survey Monkey. Please take my survey and
help me gather information I can use in my class and to help update the
productivity standards and staffing benchmarks for our Histology labs.
https://www.surveymonkey.com/r/8HFSY3V
Thank you and th
Hello Netters,
Thank you for taking my survey. I will use your replies for my research
project and I will also let everyone on the list know the results as well.
From what I've found, the surveys performed in the past asked similar questions
but more at the institution level. I would like to ge
Hello Netters,
I am taking a research techniques class for my MBA this term and need to have a
project that I can ask quantifiable questions. I was thinking about
productivity and benchmarks in the Histology and IHC labs.
If I send an email with my questions, will you be kind and answer them in
Hello Netters,
I am taking a research techniques class for my MBA this term and need to have a
project that I can ask quantifiable questions. I was thinking about
productivity and benchmarks in the Histology and IHC labs.
If I send an email with my questions, will you be kind and answer them in
Good Morning Netters,
This is a test to see if I can get an email through using this email account.
My posts haven't been making it through and I know you miss me.
Sincerely,
Paula
Paula Sicurello
Histotechnology Specialist
UC San Diego Health
___
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