Hey Histonetters,
Has anyone noted negative staining with the anti-human hepatocyte antibody
Clone OCH1E5 on hepatocytes surrounding the centrilobular vein? We are staining
mouse liver samples and get great staining on about 80-90% of the hepatocytes
in normal liver but then see very low to
! This project has been hell!
Thanks,
Julie
Message: 21
Date: Wed, 2 Nov 2011 08:46:28 -0500
From: Beckham, Sharon s...@stowers.org
Subject: RE: [Histonet] Luciferase IHC
To: 'O'Donnell, Bill' billodonn...@catholichealth.net, Kim Donadio
one_angel_sec...@yahoo.com, Randolph-Habecker, Julie
Has anyone had good results staining for luciferase in FFPE tissue? If
so, what antibody did you use.
Thanks!!
Julie
Julie Randolph-Habecker, Ph.D.
Director, Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave N, DE-360
Seattle WA
Folks,
I am looking for some standard turnaround times for histology services
including processing to embedding, processing to HE, and processing to
HE and IHC. Does anyone have that data?
Also, how do other folks handle standard turnaround time for research
samples? We may get anything
Folks,
I am looking for a control for some Flag-tag IHC I am doing on FFPE
tissue. I was wondering if someone could share some slides off of a
block of flag transfected cells.
THANKS!!
Julie
Julie Randolph-Habecker, Ph.D.
Staff Scientist - Director
Experimental Histopathology Shared Resource
Folks,
It's my annual inquiry as to whether someone has optimized
immunohistochemistry for CD4 and CD8 on formalin-fixed paraffin mouse
tissue. Over the years we have tried lots of different antibodies but I
am wondering if there is anything new that we have not tried. Any leads
would be great!
Folks,
I need some input on a problem we're seeing with some mouse brain
samples. The samples are from new born mice P0 to P14 which have been
fixed in 10% NBF (Fisher brand and well within expiration date) for 72
hours. They are then transferred to 70% etoh and processed on an 8 hour
process.
