Hello all,
The School of Veterinary Medicine at the University of Wisconsin, Madison has a
full-time position for a Necropsy Technician. The position is responsible for
assisting the veterinary pathologists in the biopsy and autopsy service - much
like a human hospital but with a much wider
Hello,
We have two positions open in anatomic pathology at the School
of Veterinary Medicine at the University of Wisconsin, Madison, the links are
below...
https://jobs.hr.wisc.edu/en-us/job/509270/histology-technology-supervisor
(application deadline June 10th)
Hello,r
Has anyone has used printed labels successfully on cassettes that
can withstand xylene/paraffin processing and long term storage? (I am NOT
looking for a cassette printer...)
Thanks!
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of
Hello all,
The handle snapped off our RM2255 microtome block holder, and
the replacement we were sent does not open the clamp far enough. (We are
currently managing by using an allen wrench in the drilled out hole...) Does
anyone have any suggestions?
Cheers,
Sandy
Sandra J.
Hello all,
Our lab is looking to replace our 10 year old VIP6. We process
anywhere from 50 to 200 cassettes daily. Any recommendations are appreciated.
Thank you,
Sandra
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine
Hello all,
Actually it's a necropsy question for those of you who work in
veterinary medicine. We perform necropsies on a very large variety of
vertebrates that require removal of the spinal cord. (From hedgehogs to
bovines.) What equipment are you using to remove spinal cords
Ho-ho-ho all,
I'm looking for a deep water bath, (3" minimum), that has an
interior light source. Most of them seem to be only 2" tall, or have a slanted
front control panel that none of us in the lab want. Does anyone know of a
brand of flotation water baths that are 3" deep
Hello,
Can anyone comment on the current formalin substitutes Excel
PLUS and/or FineFIX?
-Cost
-Benefits
-Drawbacks
-Performance in tissue processors
Thanks!
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine
Hello all,
Does anyone have a source for oversized coverglass? (35x50,
35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab,
some of which are from horses, cows, etc. that are put on the larger slides.
Fisher used to carry their own brand, but it's
Hello all,
Has anyone had experience using the Galileo Series 2 microtome, made by Diapath?
Thanks!
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine
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Histonet mailing list
Hello all,
Our only motorized Microm microtome is slowly dying, and I'm
looking for a replacement that can be used manually, semi, or fully automatic.
I'm leaning towards Leica as I have had good luck with their manual microtomes.
Please, experienced histotech
Hello,
We had to switch sources (to Novus) for Thyroglobulin
TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet
suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and pH9
EDTA AR, to no avail. Any suggestions?
Thanks!
Sandy
Sandra J.
Hello all,
The block clamp lever of one of our Leica RM2235 microtomes
snapped off. The broken end is still in the (threaded?) hole. Any advice or
source for fixing/replacing this is most appreciated.
Thanks,
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
Hello all,
I have 1 inch thick cross sections (band saw) of calf hooves.
The pathologist has already decaled the bone, and now we have to soften the
hooves so she can take thinner sections for processing.
* Which is better, potassium hydroxide or sodium hydroxide, and
Hello all,
Do any of you have to cut any of you antibody controls "fresh"?
We currently cut the MUM-1 fresh, and the Synaptophysin. (We seldom do a
Syanpt., and have found the fresh cut patient tissue has been positive while
the not-so-fresh positive control slide is negative.
Hello all,
Does anyone know of a company who buys used Histology
equipment? Our Lab Vision 720D Immunostainer still works fine, and had a full
PM in July 2018. (We purchased a new IHC stainer that is better suited for
research.)
Cheers,
Sandy
Sandra J. Cheasty, HT (ASCP)
Hello all,
Does anyone have any ideas on de-mineralizing an
ortho-keratotic, mineralizing, odontogenic cyst? It is so hard the pathologist
cannot cut it into small enough pieces to fit into a cassettes. (It's been in
decal for days with no change.) I was thinking of taking one
Hello all,
Our Sakura tape coverslipper is doing something I've never
encountered before - the lever that holds the blade and goes up and down and
cuts the tape, has gotten sticky and slow. It will chop down fine, but has a
hard time returning to the up position. Before I take
Hi all,
Does anyone know where I can get my hands on some PT module
reagent level sensors? (The black bar on the back of each tank.) Our pH 9 AR
destroyed it in one tank, and the pH 6 sensor now has some wear and tear also.
Otherwise the PT module works great.
Thanks!
Sandy
Hello all,
Does anyone know where I can get CD204, Anti-MSR1 monoclonal,
clone SRA-E5?
Thanks!
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine
___
Histonet mailing list
Hi all,
It seems Fisher has stopped carrying the "Ultra Clean Diluent
125ml". (Item TA-125-UC). Can anyone recommend someone else who carries it, or
a likely replacement? We use the Po-Link 2+Broad HRP detection kit.
Cheers!
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology &
Hello all,
We are in the market for a new IHC stainer. (Our Lab Vision 720
is no longer reliable.) We average about 20 slides a day, but sometimes get
research projects that are 50+ slides. Not interested in a closed system; it
is for a veterinary histology lab, and we need the
Hello all,
Thanks for everyone's response to error 90 on our Prisma
stainer; it has been cleared! Does anyone use filtered, softened, or DI water
in their H stainer to avoid clogging up the system?
Thanks!
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
Hello all,
We have been getting an occasional, (maybe once every 20 runs),
error 90 on our Prisma stainer. (Vacuum Breaker Error) Has anyone encountered
this? Does it have to do with the incoming water lines? I'm trying to avoid a
costly visit by a Sakura vetted repair tech.
Hello all,
I am in need of some parts for our Lab Vision 720-2D IHC
stainer that don't cost an arm and a leg. (Plunger, plastic piece that holds
the wide top of the plunger, tubing, stopcock, connections.)
If you e-mail me, I can send a picture of exactly what I'm
Hello all,
Can someone recommend an economical disposable blade for
grossing eye globes? Sharp enough to slice through sclera smoothly, strong
enough to finish the cut through the lens.
Cheers!
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School
Hi all,
We are having difficulty sectioning mouse tissue, (brain,
spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has anyone
had issues with paraformaldehyde fixed tissue? They were processed routinely,
starting in 10% NBF, with other tissues, and we are
Hello everyone,
Does anyone know of a company that sells the cardboard drawers
and drawer dividers for filing blocks, separate from the outer cardboard file
that the drawers slide into?
We came into possession of 25 outer files with no drawers,
which means we
Hello all,
We use the "Supa Mega Clamp" (Ted Pella) for sectioning large
tissues in the oversized cassettes. Lately, it seems not to grip as tight as it
did when we received it a month or so ago. The literature is little more than
photos of how to attach the clamp to our
Hello,
Can anyone recommend a band saw for pathology? We are looking
for a new band saw for use in necropsy and research. It has to be a "meat" band
saw, as workshop band saws aren't compatible with wet tissue.
A table-top model is preferred, and cheaper than a floor model.
Hello all,
I am looking for opinions on giant block clamps for the Leica
RM2235 and Microm HM355S microtomes.
* I know CellPath makes one, are their others?
* Is it easy to swap them out for a regular clamp, or is it advisable
to dedicate one microtome for these
Hi everyone,
I have a newly refurbished Leica RM2135 at my disposal, but the
knife holder is for high profile blades. 2 questions.
1-Is it that different using high-profile blades vs. low. (I have used
low-profile blades forever.)
2-There is a slot on the bottom of the blade
Hello all,
Can anyone recommend an economical sharps disposal container
for long plastic pipettes?
Cheers,
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine
___
Histonet
Hello all!
Does anyone have a source for Thyroglobulin Ab-6 (Clone
TGB04+TGB05), 200ug/ml? We use it primarily on canine and feline, (It also
works on guinea pigs,).
Thanks much!
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary
Hello,
We have 2 Microm HM355S motorized microtomes. We use the motor
to move the block close to the blade, but turn the wheel manually when
sectioning. Both are "jumping" forward on their own occasionally while
sectioning, which is unsettling and chunks into the block. One of
Hello all,
Picasa, the program we currently use for re-naming and
organizing our gross digital photos, will no longer be supported by Google. If
anyone can suggest another program for this purpose, please let me know. We
don't need any fancy editing of the photos themselves
Hello All!
We are looking for a dedicated Histologist, with at least 1 year of experience
in a full service histology lab, to join our Veterinary Pathology team. Our
Histology lab serves the Veterinary Medical Teaching Hospital in Madison,
Wisconsin, as well as many research needs at the School
Hello all,
Has anyone using Richard Allen Hematoxylin-2 noticed an odd
artifact on the slides after using the Hematoxylin for more than a few days on
their stainer? We are seeing small spore or pollen-like blue dots here and
there on the slides. It is not coming from the water
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