I'm trying to locate aniline oil for the Holzer glial fiber stain. I
purchased what was listed as aniline oil from ENG, but the first hint that
something was wrong was that it was incompatible with the chloroform also
included in the differentiating solution. Looking at the fine print on the
Sorry, Freudian slip, of course I meant Advance for Medical LABORATORY
Professionals! Sometimes my "hats" get mixed up... thanks, Susan
-Original Message-----
From: Susan Bachus via Histonet
Sent: Saturday, September 03, 2016 1:12 PM
To: histonet@lists.utsouthwestern.e
I'm trying to find a pdf of this paper for my students. I have an ancient
battered xerox copy in shades of gray. For some strange reason it's not in
the Advance Medical Library Professionals online archives. I'm hoping that
Stephanie is in Histonet, or that someone else has access to a copy.
You got me to thinking, that I would be in a real jam if I ever lost mine!
I'd rather buy one from someone who has old ones they don't need any more,
but I did find some (at least I think this is what you're describing)
available online at:
http://www.psl-equip.com/shop/advanced_search_result.p
This may sound simplistic, but it works for us: whenever I need to use pH
something, I quickly first dunk the electrode in the buffers above & below
what I'm pHing (rinsing after each dunk, of course)--if the meter reads
accurately I proceed to measure, if it's off by more than a few % I
reca
I have an old surplus Mettler balance that is no longer worth recalibrating--I
hate to waste anything & am wondering if anyone might find it useful enough for
a display or something like that to be worth the cost of shipping?
___
Histonet mailing list
H
I have routinely seen that we obtain slightly different results (like 10-20%
variance) in using 35S dATP for TdT-catalyzed tailing reactions of
oligonucleotides from month to month as we use different batches (made fresh
monthly) of the 35S dATP. But on a few occasions over the years we have
Sorry to be so late to the party, hope I'm not too late to share my
story: I still vividly remember being shown a "career documentary" film
about histotechnology, in junior high school, back in the 60's, by my
biology teacher, Lynda McCurdy Ballingall--the lovely lady who influenced my
li
Good old fashioned gelatin subbing is very cheap & works great--I have never
seen tissue separate from a subbed slide! Susan
- Original Message -
From: "Peggy Bisher"
To: "Milne, Katy" ;
Sent: Friday, June 19, 2009 3:34 PM
Subject: Re: [Histonet] Superfrost plus gold slides vs super
Protocol #3 at this site is great for RNA probes:
http://intramural.nimh.nih.gov/lcmr/snge/Protocols/ISHH/ISHH.html
This protocol assumes fresh frozen tissue but can be used on fixed, paraffin
embedded tissue--obviously you would have to include a step at the beginning
to deparaffinize. You mi
There's a wonderful company called Oligo's, Etc. (Wilsonville, OR) that
synthesizes very affordable oligoprobes. But you have to supply the
sequence information. Susan
- Original Message -
From: "Sharon Campbell"
To:
Sent: Monday, January 26, 2009 9:20 AM
Subject: [Histonet] ISH pr
As long as they are
kept dry, they should be good for years.
- Original Message -
From: "Susan Bachus"
To: "Neil Fournier" ;
Sent: Tuesday, December 30, 2008 6:31 PM
Subject: Re: [Histonet] subbing slides
This always works like a charm for us and costs ~10x
This always works like a charm for us and costs ~10x less than buying
pre-subbed or electrostatic or whatever fancy expensive store-bought slides!
Have fun! Susan
- Original Message -
From: "Neil Fournier"
To:
Sent: Tuesday, December 30, 2008 6:21 PM
Subject: [Histonet] subbing sl
I tried earlier to send, for this thread, a wonderful paper by Nauta,
chronicaling the history his discovery of his tract tracing method, in which
serendipity and degradation of formalin played critical roles, not realizing
that the size of the attachment would prevent it from going through, so
I believe that the "swiss cheese" holes are due to ice crystal formation
during freezing, at least that's the rationale we were always taught for
using additional fixation in sucrose-formalin after the initial fixation in
formalin, i.e. the sucrose would prevent ice crystal formation. Susan
--
Here's one potential problem with listing real names: a few years ago a
very diligent student of mine inadvertently stirred up a lot of controversy
when she asked for advice from Histonet on a class assigment and someone was
concerned that she was "cheating". I had in fact recommended Histone
Back in the old days we used to use "india ink" to label slides--so I don't
know whether it would also work on tissue (as opposed to glass), but it
might be worth a try. Susan
- Original Message -
From: "Gudrun Lang" <[EMAIL PROTECTED]>
To: "'Leslie Chen'" <[EMAIL PROTECTED]>
Cc:
Sen
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