[Histonet] Subject: braf (v6001e)

Hey Charles, I am sure you have thought about this but your retrieval process could be too stringent if you are having over expression. Thanks, Timothy Higgins, HT(ASCP), QIHC(ASCP)cm Histology Supervisor Center for Disease Detection/LabCorp tim.higg...@cddmedical.com Message: 6 Date:

[Histonet] Subject: Re: Trouble shooting question

Hey Charles, I have to agree with Rene. They are either being left out after sampling prior to fixation or possibly coming into contact with air during the processing run. Make sure your solution are covering all the cassettes, don't let them float free inside the processing chamber.

Re: [Histonet] Antibody Validation CLIA

Cap states, "should test a minimum of 10 positive and 10 negative tissues". Does not state "cases" but "tissues", so we used a block for positive and negative from the same case. Thanks, Tim -Original Message- From: histonet-requ...@lists.utsouthwestern.edu

[Histonet] RE: Buffer pH monitoring

I check pH every time I make a new batch(bottle) and then periodically there after. Timothy N. Higgins, HT (ASCP), QIHC Message: 10 Date: Tue, 11 Feb 2014 16:23:22 + From: Gupta, Nilesh ngup...@hfhs.org Subject: [Histonet] Buffer pH monitoring To: 'histonet@lists.utsouthwestern.edu'

[Histonet] RE: Ventana LEAN

They come in, give their opinions on how to make your workflow better. I didn't get much from it, we didn't make any changes, more of a selling tool in my opion. I guess if your workflow was terrible, they might be able to offer good advice. On a positive, they were all very nice. Thank,

[Histonet] FW: Fungus Controls

I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its naturally occurring, a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does

RE: [Histonet] edge effect on IHC

Hey Mary, I would suggest observing your stainer to ensure the proper drop zones are being performed and not just dropping closest to the label. I used Dako for years and loved them but you do have to make sure it dispenses properly from time to time. They use drop zones unlike Ventana which

[Histonet] Dimwits

Hello Annieinarabia, You are quite the advocate for Sir Rene J. of the most respected voices on this forum. Truly if you want to be respected, give respect and do not degrade other as he does from time to time (in my opinion). Give substance in your responses and not comment using some

[Histonet] RE: Histonet Digest, Vol 118, Issue 23

Hey Martha, You have to forgive Rene J. she speaks sometimes and has nothing good to say but keeps speaking. There is never a bad question, if you don't have a good answer then keep it to yourself as I usually do. Adequate forum. really? I guess no one on Histonet is allowed to share

[Histonet] Batch Controlling for IHC

Hello All, Have you ever had a Pathologist say, if you have one tissue control (placed on the same slide as the patient tissue) per antibody, per patient case, but there are multiple blocks (same tissue type) from the same patient case, that would be batch controlling? Hope that made

[Histonet] RE: High complexity test

Hey Kathy, It is not considered a High Complexity test on the staining side of it. You don't need any special degree or certification, I know we all want our profession to fetch more money and respect and a good way is make a mountain out of molehill to use an old saying. Now here is

[Histonet] MOHS

I need some MOHS procedure help, anyone want to help me offline? I would really appreciate it!! Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Re: Question

All I have to go by is CAP's response to the question, Is IHC a High Complexity Test. Their verbal answer is no, but there is nothing formally documented. The only documented statement is from 2010 Webinar during the Question and Answer portion. It is as follows, Laboratory Accreditation

[Histonet] Re: Question

The professional interpretation is considered a high complexity test but not the actual technical component. Thanks, Timothy N. Higgins, HT (ASCP), QIHC ___ Histonet mailing list

[Histonet] interview

Gale and Pam, people on Histonet love to sit on their soap box and spew out ridiculous statements without any thought behind them at times. To say you need to scale the volume down to a point where it is manageable (tell that to your boss!! Whatever!!) is coming from someone who

RE: [Histonet] interview

posting a problem. At least that is what I always try to do! René J. From: joellewea...@hotmail.com joellewea...@hotmail.com To: Tim Higgins thiggin...@msn.com; histonet@lists.utsouthwestern.edu Sent: Thursday, January 10, 2013 11:07 AM Subject: Re: [Histonet] interview You can always delete

Subject: [Histonet] Background staining on H pylori

Hey Deloris, I would have the dispensing nozzles on the XT checked, could be it is not getting rinsed properly. Had that issue with my Ultra not to long ago. No warnings or errors showed up, just a clogged nozzle tip. Tim -Original Message- From:

[Histonet] Re: Dako vs. Ventana IHC systems

Nobody has mentioned it, but can you imagine the amount of revenue the manufactures would loose if they came out and supported the decision on not using negative controls. That’s not going to happen!! I have used both systems the Dako and the Ventana (Benchmark, Benchmark XT, Ultra) and both

[Histonet] Re: Cutting Blocks

All great points, awesome information, that’s why we all enjoy histonet. One thing I've learned over the 20 plus years in histology is every person is different and you cant stick them all in the same box. I personally don't believe 40 blocks an hour is unreasonable as an average as long as

[Histonet] blocks and slides

Hey Dorothy, It's 10 years for CAP, but you need to check with your state as well. I know for New York you must hold them for 20 years. Thanks, Tim Message: 20 Date: Tue, 11 Sep 2012 08:09:24 -0700 (PDT) From: Dorothy Glass techman...@yahoo.com Subject: [Histonet] blocks and slides To:

[Histonet] Re: Frozens and antigen retrieval

Agree, only if the antigen has been bound by a fixative. -Original From: Daniela Bodemer daniela.bode...@mcri.edu.au To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 4, 2012 7:02 AM Subject: [Histonet] Frozens and antigen retrieval Hi all, A

Subject: [Histonet] nuclear fast red

Nuclear Fast Red needs to be heated during prep and it does take a little while to dissolve, it never really dissolves entirely, probably saturates the solution pretty fast. Tim Message: 1 Date: Tue, 29 Nov 2011 11:29:07 -0700 From: Patsy Ruegg pru...@ihctech.net Subject: [Histonet] nuclear fast

[Histonet] Spirochete Control Block

Hello Histonetters, Anyone have a spirochete control block(s) they would like to trade? Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Histostar Embedding Station

Hey Jessica, We have two Histostar's that have been here since March and have not had any issues with them. Can't say anything bad about them, yet. Thanks, Tim Subject: [Histonet] Histostar Embedding Station To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID:

[Histonet] DISPOSAL OF SPECIMENS

I have been in the histology field for 20 years and never heard of having to remove the labels prior to disposal through an approved vendor. I don't believe that the labels from blood tubes are removed prior to disposal, this would be the same type of scenario. Thanks, Tim Message: 9 Date:

[Histonet] p57 antibody

Hey Histonetters, Does anyone use the p57 (kip 2) antibody from Thermo (Lab Vision)? I am still having trouble with the pathologist saying it's not staining the syacitiotrophoblasts. Any help with a protocol or even me being able to send in a slide that you could stain and send back to be

[Histonet] p57 kip2 (Ab3) concentrated antibody

Does anyone use the p57 (Ab3) concentrated antibody from Lab Vision (Fisher) for Molar pregnancies on the Ventana platform using the UltraView detection system? What is the protocol and dilution you run? Any help would be greatly appreciated!! Thanks, Timothy Higgins, HT(ASCP), QIHC

[Histonet] Tissue handling at embedding

I have worked at labs that reuse molds and ones that clean between uses. Guess it all depends on what your definition of a clean mold is. If you have the time and space, not reusing the molds is best practice but not always practical. Thanks, Tim Message: 10 Date: Tue, 20 Sep 2011 09:44:26

[Histonet] STP420ES

Good instument, been working with it for about 6 months, using the Fast Flex system for rapid tissue processing. What did you want to know specifically? Tim Message: 8 Date: Tue, 13 Sep 2011 09:11:53 -0700 From: Davide Costanzo pathloc...@gmail.com Subject: [Histonet] STP420 To:

[Histonet] IHC validation

Hello Histo Friends, Has anyone had to validate IHC antibodies when you do not have tissue processed in house to use for your validation. I do have control tissue that stains properly but it was acquired from outsides sources with verified proper predicted staining. If you have done this

[Histonet] RE: Re:peggy wenk comments on HT/HTL practical

I have to agree with the fact that students should not be released into the workforce without any practical experience. We team up with a local college (St. Phillips College, San Antonio TX) that produces several students a year and we along with other facilities help in the rotations for

[Histonet] RE: Re:peggy wenk comments on HT/HTL practical

How would the expression, you need experience to get the job, and you need the job to get experience work if a person went through flight school to be a pilot but did not receive any actual flight training but was then allowed to get a job as a pilot flying a commuter plane in charge of peoples

[Histonet] Re: Recycled alcohol

Hey Michelle, The easiest way to find out if it's the recycled regeants is to use fresh reagents and see how the stain looks. Obvisously if it still shows sign of a lack of color or detail than I would think it could be the stains themselves. What manufacture and type of Hematoxylin and Eosin

RE: [Histonet] Controls with patient specimen on same slide

It is the most effective and economical way to run your IHC's. We have been doing it that way for as long as I have been in histology. Thanks, Tim - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 19, 2011 6:04

[Histonet] Re: Histonet Digest, Vol 92, Issue 18

On all stomach biopsies. Tim - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 13, 2011 10:45 PM Subject: Histonet Digest, Vol 92, Issue 18 Send Histonet mailing list submissions to

[Histonet] Control Tissue

My apologies, I wasn't ready for the number of responses I received over the control tissue and might have missed some people. If I didn't contact you back shoot me another email so I can start working on it. Control Tissue still Available Iron/FE Tonsil Hpylori CD15/30 TTF1 p16 AFB

Fw: [Histonet] Re: Embedding Media

Last comment on the paraffin conversion for me. You can use bees wax if you want in your processor and embedder that doesn't mean you going to get the optimal results for every situation. Paraffin types are a personal preference for most people or labs. It's a little more hassle to keep

[Histonet] Re: Embedding Media

Hi Paula, Obviously there are no compression free paraffin's on the market. Look for a paraffin with a higher polymer content, this makes the paraffin firmer and less likely to compress. Other factor for into section compression but paraffin is a good place to start. The higher polymer

[Histonet] Tissue controls

I have an abundance of H-pylori controls that I would be willing to part with and a number others as well. Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [IHCRG] RE: [Histonet] batching controls

What is the purpose of batching control? Unless you are having a hard time finding certain positive tissue types, it's good practice to run a positive control with each case to ensure every slide shows the desired results. Plus the patholigist have to call and verify that the control worked,

[Histonet] (no subject)

Hi Histonetters, This maybe a stupid question but I'm going to ask anyways. Is it legal to buy tissue blocks (with human tissue) from another lab to use as controls for specials and Immunos? I know we purchase slides from vendors to use with our specials and Immunos. Thanks! Tim

RE: [Histonet] mislabeling of slides and blocks

Glad I never worked at that lab, never would have made to a supervisory role. I have to agree with Sarah, two infractions in a 12 month period is extremely severe. I don't care if you have Vantage or any one of the million tracking software packages out there. Especially with the shortage of

RE: [Histonet] problems with staining IHC

Hi Mala, We've used the Fisher superfrost plus slides for years and have never had that issue, it sounds more like you need to add some Tween to your buffer. That will help the solutions spread along your slide. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original

RE: [Histonet] Marking inks

We use Statlab and Fisher marking inks. We dip our specimens in Bouin's or Vinegar to set the ink. We don't have any problems with the ink coming off. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] change in immuno machines

Agreed. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, October 01, 2010 12:27 PM To: histonet@lists.utsouthwestern.edu Subject:

[Histonet] Ventana Ultraview DAB detection kits for sale.

Hello Histonetters, We are overstocked on Ultraview DAB detection kits and looking to sale some for $2,300.00 per kit. We will pay for shipping within the continental US. The kits are NOT registered with the button still attached. We have 6 with the expiration Date: 4/2011 Lot #812955

RE: [Histonet] RE: Questions

Agreed! Thanks, Timothy Higgins, HT(ASCP) QIHC Histology Manager APA Amarillo, TX -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, September 20, 2010 10:33 AM To: Senn, Amy

[Histonet] Ventana Ultraview Detection

Hello Histonetters, We are in the process of selling some of our Ventana Ultraview Detection kits. The kits still have a long shelf life and have not been registered. If you are interested in purchasing one of more of these kits please email me with questions and or contact info. Thanks,