[Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background)

Hello Histonetters,I am writing this to seek your help regarding a very recent problem that I am currently facing with Picro Sirius red staining of lab animal (mouse and rat) liver samples. I follow the procedure that was provided by John Kiernan in the histonet archives (please see below),

[Histonet] Troubleshooting Gomori's Trichrome Stain (Blue Collagen, Richard-Allen) staining

Dear histo experts,Please provide me with your valuable suggestions for the problem described below.Objective: Staining rat hearts (fibrosis) with Gomori's one step Trichrome Stain (Blue Collagen, Richard-Allen) to demonstrate the collagenProcedure: Paraffin sections of 4 micron thickness;

[Histonet] Import of staining reagents(richard allan/microm, now thermo)

Hello histonetters, This is with regard to import of Richard allan(microm, now thermo)staining reagents from US to India. After facing several problems from stains manufactured by local manufacturers, we thought of switching over to RA stains. The local distributor here is telling that these

[Histonet] Your expert advice please_histology systems

Dear Histonetters, We have planned to procure one set of histology systems for our laboratory(We have the existing one set of instruments all from Leica). We are considering the purchase of Tissue-Tek® VIP® 6(sakura) for tissue processing and Robot-Stainer HMS 740(from microm). Any of our

[Histonet] Oil red o_Dextrin_reg

Dear Members, I am trying to standardize the oil red o staining method in murine frozen tissues using Churukian method. I am having following queries. 1. The type and source of aqueous dextrin used in the staining procedure. Search in sigma catalogue provided different types of dextrin (dextrin

[Histonet] Snap freezing_method by John Tarpley_reg

Dear Histonetters, This query is with regard to snap freezing technique of rodent liver for cryotomy. Thanks a lot for the extensive details available with our forum especially from Gayle Callis. But I have seen numerous quotes regarding the use of the method from John Tarpley, who published

[Histonet] Any help for this oil red o paper

Dear histonetters, Can anybody will help me with the following oil red o paper from Charles Churukian. Journal of Histotechnology, 22(4):309-311 Charles Churukian, Lillie's Oil Red O method for neutral lipids. I am not able to access the NSH site,the following error message is coming.