Re: [Histonet] curling of kidney sections in water path

There is two things I do for kidney and liver tissue. 1. Add 5ml glycerol to the 100% Alcohol in the processor 2. After I face the block I soak the block in a dilution of liquid fabric softener. 40% fabric softener 60% water. If the first thing doesn't fix the problem then I move on to the sec

Re: [Histonet] canine teeth

I would decal with TBD-2 for about two weeks. TBD-2 is a formic acid decal solution. I would shake the specimen 24 hrs a day. Vacuum and release the specimen several times a day. Change the decal every couple of days. Do not remove the paraffin it helps to protect from over decalcifying.

Re: [Histonet] staining of plastic sections

I use McNeal as a substitute for H&E. I use it on MMA embedded bone. It is to intense (for us) to be used on JB-4. I think you might need to try a few different stains to select the best. */ /* */-- You are what you do - not what you say --/* */Karen Bowden Staff Research Associate II Univ

Re: [Histonet] 100 micron sections

Francisco *From:* kbowden *Cc:* histonet *Sent:* Wednesday, July 22, 2009 4:46:31 PM *Subject:* [Histonet] 100 micron sections Hi, I am being asked to section paraffin embedded fat at 100 microns. I haven't sectioned anything in paraffin t

[Histonet] 100 micron sections

Hi, I am being asked to section paraffin embedded fat at 100 microns. I haven't sectioned anything in paraffin thicker than 20 microns. What are the tricks to get it to unroll in the water bath? -- */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Ortho

[Histonet] Fat histology

I work with bone and connective tissue. I have just been asked to process and section some fat. I would like to get some information on how to work with fat. Any information will be very helpful. -- */-- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthop

[Histonet] Opinions: motorized microtomes Thank you!!

RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. kbowden wrote: I'm looking to buy a motorized paraffin microtome. I have been looking to see what is out there, and there seems to be quit a few. I would li

[Histonet] Opinions on motorized microtomes wanted

I'm looking to buy a motorized paraffin microtome. I have been looking to see what is out there, and there seems to be quit a few. I would like to get feedback from people who have used them. I have a problem with my shoulder and the continuous motion of using a rotory microtome is a issue f

Re: [Histonet] Bones

One of the departments (body donations) here uses bugs to clean soft tissue off of bones. I thinks it takes about a week or so. You might look into the type of bugs you have in your region for that purpose. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of O

Re: [Histonet] citrisolve?

I have used Citrisolve for serveral years. I don't think anything compares to Xylene, but I do think this comes very close. I change it about once a week. It pretty much deparaffinizes in the same amount of time. Disposal is dependent on the regulations in your area. When I started using i