[Histonet] Histoline company

Dear All we have a limited fund for buying new automatic microtom. we have got offer from an agent for model RM3600 histoline company - Italyhave any one worked befor with such model or any recommendations please thanks ___ Histonet mailing list

[Histonet] Rabbit and chicken eye processing

Dear all What is your optimal protocol for processing rabbit and chicken eyes ? and do you prefer to cut it 2 halfs or inject it with NBF 10% or whole processing is the best ?? Thanks in advance Mohamed ___ Histonet mailing list

[Histonet] clearing time

Dear all what is your optimal clearing time in xelene for prostate and tests of dog samples (about 0.5 cm) ??is there a need for additional clearing in methyl benzoat ? thanks Mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] unsolved proplem

Dear allI have submitted a proplem about reprocessing tissue blocks that have patches of stained areas and unstained pale yellow areas (H stain)!!!, unfortently the proplem didn't solved yet and i have tried all possible solutions, increasing processing time and ordered new chemicals from other

[Histonet] tissue reprocessing

Dear all I have couple quesions please 1- is there a protocol for reprocessing of mice kidney and liver after paraffin embedding due to insufficient processing time? 2- Is there a formula to add to processing agents as softening agents? thanks Mohamed

[Histonet] curling of kidney sections in water path

dear all, i'm facing an issue that trouble us when we process tissue samples of different organs and cutting them; all organs are ok and spread fine and somoothly on ater path except for kidney sections (looks wrinkled and curled as if the praffin fram around it prevent it from spread!!!). i

[Histonet] paraffin dispenser help!

Hi all i'm in urgent need for operating SHANDON LIPSHAW PARAFFIN DISPENSER MODEL 224 how to start using it please in simple stepsthanks in advance mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] our first histology conference

email me for arrangment all advice from you well be appreciated thanks   Dr. Mohamed abd El Razik Ass. Lec. of Vet. Histology Faculty Of Veterinary Medicine Cairo University - Egypt http://www.facebook.com/mohamed.abdelrazik.9 ___ Histonet mailing

[Histonet] RE: ammonium hydroxide

program   hope that help   Mohamed Abd El razik Ass. Lec. of Cytology Histology Faculty of Vet. Med. cairo University- Egypt From: Rathborne, Toni trathbo...@somerset-healthcare.com To: 'Canal, Erica' eca...@hei.org; histonet@lists.utsouthwestern.edu histonet

[Histonet] lab. setting up

dear all on histonet I'm going to setup a new histology lab. in Egypt. I need to contact with you to advice me about the best and comfotable facilities microtomes- processors-automatic stainers and so on .   Mohamed Ass. Lec. of histology Faculty of Vet. Med. Cairo University Egypt

Re: [Histonet] Re-send Darker /or granular hematoxylin staining (no photo)

I think these granules are of insoluble hematoxylin or preceptated hematoxylin granules a had these condition 2 years ago and resolved it by prepairing new one. hope these could help   Mohamed Abd el Razik Ass. Lec. of veterinary histology Cairo University- Egypt

[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] which conferences?

dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and a link if avaliable   thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] scholarship

hi all i'm going to apply for PhD degree in using educational technology in Histology teaching. do you know any university i could contact for acceptance letter? i have my funded scholarship   thanx ___ Histonet mailing list

[Histonet] why no replay????

thanx to all kind replays from sgoebel, Paula Pierce and JR R. but i know that Alcian blue 2.5 stain most acidic mucins and pH 1 stain sulfated one. my quistion is:  i found in several serial section on intestine that 100 goblet cell react to AB ph 1 and only 50 cell reacts to AB 2.5 why??

[Histonet] why no replay??

i have posted my quistion 2-3 times several days ago and recieve no negative or positive comment !!! why?? is my quistion not clear or what?? --- On Thu, 6/2/11, mohamed abd el razik k8...@yahoo.com wrote: From: mohamed abd el razik k8...@yahoo.com Subject: Alcian blue quistion To: histonet

[Histonet] Alcian blue quistion

hi every body i have a confusing quistion reagrding Alcian blue pH 1 2.5 stains. suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these

[Histonet] Alcian blue quistion

hi every body i have a confusing quistion reagrding Alcian blue pH 1 2.5 stains. suppose you have 100 goblet cell react positive to pH 1 which mean all have sulfated mucin. and 50 cell from other section react positive to pH 2.5 which mean acidic type(sulfated or sialated). why these 50 cell

[Histonet] chicken immunohistochemistry

dear all i'm looking for vendors or companies that we could order some anti chicken antibodies (primary secondary). speacially for CD4 CD8 i'm new in that feild and need your help.   mohamed   ___ Histonet mailing list

[Histonet] elecron microscope needed

hi all i miss you several days. we are medium research lab and looking forward to bring scanning transmission electron microscope . so which manufacture or brand and model you recommend us and average price ?? any offers are welcomed to my mail    thanx in advance   mohamed

[Histonet] pituitary stain

--- dear all can you share me pituitary stain protocol called PAS/ celestine blue / Orange G?   thanks in advance   mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] alkaline phosphatase

 dear all i'm going to detect alkaline phsphatase in avian intestine and i didn't do that befor so any help with protocol?? or kits for that and how to use it can we detect it in FFPT?   by the way i have sent a question twice befor about how to  diff. between red muscle and white one and have

[Histonet] red and white muscle?

Dear all how can i differentiate between Red Muscle and white one (formalin fixed)? would you mind to share your protocol   thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] red and white muscle

Dear all how can i differentiate between Red Muscle and white one (formalin fixed)? would you mind to share your protocol   thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] RE: Cutting, Processing, etc

i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its

[Histonet] how to be qualified?

cert. to work as i have master degree?? if yes i have searched there site but can't find where i can pass the exams in Egypt!! can you send me a link if you reached it ortell me how to be certified if i'm in Egypt??   thanx in advance Mohamed Abd El Razik Histology Dep. Faculty of Vet. Med. Cairo

[Histonet] please enter

Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the

[Histonet] massons question

hi all i'm going to stain some slides of small intestin which were fixed in susa. i'm going to stain it by Massons trichrome. i have read that slides should be secondry fixed in Bouin's or saturated picric solution.   does it mean fixation of processed tissue after cutting with microtom?? how

Fw: [Histonet] HE stainers

we are going to bring new one too for our small lab. any suggestion please. you can send me at k8...@yahoo.com --- On Wed, 6/30/10, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] HE stainers To:

Fw: [Histonet] Tissue Processors

i need it too as we are going to bring new tissue processor to our small lab --- On Sat, 6/26/10, Shirley Pan sj_...@yahoo.com wrote: From: Shirley Pan sj_...@yahoo.com Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Date: Saturday, June 26, 2010, 7:55 AM We are in

[Histonet] what do you think?

dear histonetters i have a quistion please regarding the thickness of lamina propria in small or larg intestine. what is the significant of it?? does it mean inflamation and pathological condition or mean more size and so absorption of nutrients? i mean does the thickning is favorable or not?  

[Histonet] manual morphometric analysis

hi all histonetters i need to do some morphomtric analysis on some samples of small intestin but we have no image analysis software so  i need to do it manually by occular micrometer. i need to know how to calculate villus surface area? overall mucosal surface area of cross section?  surface

[Histonet] why no response??!!

dear all i have posted few days ago a quistion about enzyme significance in tissue?? eg. why i looking for alkaline phosphatase??? what it indicat??  i need a source or a sheet answering me   and i have nearly no response from you any help her??   thanx mohamed

[Histonet] enzymes significance?

dear histonetters as i'm learning HC IHC i need to know a reference or internet site that could help me to know the significance of different enzymes in tissues?? any help in these!? a second quistion please i'm trying to locat Alk. Phosphatase in chicken intestine and i found in old reference

[Histonet] fixtives

are you using bouin's , susa or formol zinker?? how many times do you use the same fixtive? i know that zinker need to be freshly prepaired   thanxs     mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Happy Easter!

Dear histonetters I wish to you, Happy Holidays with a lot of peace and health! dr.Mohamed Abd el razik Histology Dep. Faculty of Vet. Med. Cairo University- Egypt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] cryostat help please!

our cryostat is Minotome Plus Microtome Cryostat Cat. No. 2563-- For 120 VAC, 60Hz   --- On Sat, 3/20/10, Cheryl Cornett-Early cornettear...@bellsouth.net wrote: From: Cheryl Cornett-Early cornettear...@bellsouth.net Subject: Re: [Histonet] cryostat help please! To: mohamed abd el razik k8

[Histonet] RE: cryostat help

 i forget to  introduce my self. i'm  demonestrator  of histology in faculty of  vet. med. Cairo University- Egypt.(nearly 2 years experience) and i'm so happy to know you all histoneters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] cryostat help please!

hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room

Re: [Histonet] cryostat help please!

thanks for your replay ray yes i set the temp. to -20 degrees but it is 20 for the last week. --- On Sat, 3/20/10, Mark Ray darkd...@comcast.net wrote: From: Mark Ray darkd...@comcast.net Subject: Re: [Histonet] cryostat help please! To: mohamed abd el razik k8...@yahoo.com Date: Saturday