August 2018 11:22:55
To: Hobbs, Carl
Cc: histonet
Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
Hello Carl,
Alcianblue is q quite interesting stain! Maybe this selection of a presentation
helps you to find a suitable method. I attach you a PDF-document to this email
From: Mac Donald, Jennifer
Sent: 23 August 2018 19:52:52
To: Hobbs, Carl
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
The hyaluronidase is digesting out hyaluronic acid, a component found in
connective
blue
pH 2.5 and negative for pH 1.0.
-Original Message-
From: Hobbs, Carl via Histonet
Sent: Thursday, August 23, 2018 11:32 AM
To: histonet
Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide
Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab
pH1 ( highly sulphated mucins only) on a sequential slide?
Sure, instead use Alcian blue CEC method?
I will be interested to read other responses to your request.
Mucin-illy
carl
Carl Hobbs FIBMS
Histology and
Help? we have a student with one of the online schools and we're doing
specials like it is 1972. She has to do a Al Blue 2.5 w Hyaluronidase
digestion. We haven't done this since the development of clinical IHC two
dozen years ago -
Is there any other way to acheive this outcome on a colon
