Hello Histonetters,
I am looking for a good "Amyloid A control". We have an anti-amyloid A
antibody which is specific only for amyloid A. We have tried our commercial
congo red controls and they do not work. We have only had a few positive
cases. I am willing to trade control blocks. We ha
This is a well known phenomenon of amyloid staining and it is fairly
common. It is due to the fact that amyloid is a very variable material
and different samples may react differently to the dyes. I suggest you
restain using Highman's congo red method
(http://stainsfile.info/StainsFile/stain/am
2015 12:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid by Congo Red
Greetings to all Histotechs- Here's an amyloid question for the braintrust.
We are cutting our slides and controls at 9 and staining in Congo Red for 1
hour. The control stains fine but the pati
What protocol and reagents are you using for the stain?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On 1/23/15, 10:43 AM, "Jeffrey Robinson"
wrote:
>Greetings to all Histotechs- Here's an amyloid ques
Greetings to all Histotechs- Here's an amyloid question for the braintrust.
We are cutting our slides and controls at 9 and staining in Congo Red for 1
hour. The control stains fine but the patient tissue is staining negative even
on cases that the pathologist assures us should be positive fo
Does anyone out there use the Amyloid Red stain from Anatech in place of the
Congo Red dye? Due to the pricing, would like some critiques before I order
it!!!
Thanks,
Dorothy Webb
This e-mail and any files transmitted with it are confidential and are inten
Carole,
This may or may not be of help - Millipore's xAPP (MAB348) works on horses;
HIER using high pH retrieval solution. I don't have data on reactivity on
pigs yet.
Good luck,
Jan Shivers
UMN Vet Diag Lab
St. Paul, MN
On Mon, Sep 16, 2013 at 3:52 PM, Johnson, Carole wrote:
> Hello All,
>
>
Hello All,
Does anyone out there in Histoland have experience with this antibody? I have
been asked to look into running IHC on pig brain on the Bond Max platform. I am
looking into the antibodies from LS Bio and Invitrogen/LifeSciences, and would
appreciate any insight you could provide. Thank
Check/change the solution
René J.
From: Gale Limron
To: "histonet@lists.utsouthwestern.edu"
Sent: Thursday, May 30, 2013 11:26 AM
Subject: [Histonet] amyloid stain
Hello,
It's been awhile since we ran an amyloid stain.Today, using Richard
Allan Chromaview kit, ou
Hello,
It's been awhile since we ran an amyloid stain.Today, using
Richard Allan Chromaview kit, our staining tech noticed a lot of precipitant in
the working Congo Red solution. Any thoughts or advice?
Thanks,
Gale
Gale Limron, HT, CT (ASCP)
Union Hospital
Dover, Oh
This e-mail
I think this is a great article to read about amyloid. This is the link to the
paper
http://propath.com/newsletters-immunohistochemistry-173/181-september-2004-immunohistochemistry-in-amyloidosis
It belongs to a great Pathologist Dr. Rodney T. Miller
Dr. César RomeroBuenos AiresArgentina
: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid control tissue
Does anyone know where I could purchase (or trade for) a block of
amyloid positive tissue?
Alternatively, for those who purchase ready-cut amyloid control slides,
could you share what vendor you have been happy with
Does anyone know where I could purchase (or trade for) a block of amyloid
positive tissue?
Alternatively, for those who purchase ready-cut amyloid control slides, could
you share what vendor you have been happy with?
Thanks in advance!
Barb Moe
Gundersen Lutheran Medical Center
La Cros
Does anyone have a procedure to do an amyloid stain on a smear? Someone at the
university wants to see if there is amyloid in milk.
Roberta Horner HT/HTl
Animal Diagnostic Lab
Penn State University
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Yes, insulinomas can show amyloid; however, they are very rare tumors.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Off
Hi,
I was also told or read somewhere that insulinomas are good amyloid controls.
I've never had a chance to test any blocks from these cases. What has your
experience been? Thanks for your help.
Carrie
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I find IHC for amyloid A (AA) helpful only in kidney; however, it is not
requested often.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 0610
inal Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew
Meyer
Sent: Monday, April 05, 2010 1:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid Stain
Hey Guys,
I was hoping to get some help with
Hey Guys,
I was hoping to get some help with the best way to approach a request for an
amyloid stain. The only thing I've ever done in the past is the traditional
Congo Red... however we were wondering how the Amyloid A Immuno stain is.
Our pathologists were wondering if the Amyloid A is more/les
The method at the address below should work on cryostat sections.
http://stainsfile.info/StainsFile/stain/amyloid/siriusllew.htm
Bryan Llewellyn
- Original Message -
From: "Tony Henwood"
To: "Sharon Allen" ;
Sent: Wednesday, August 19, 2009 3:26 PM
Subject: RE
Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon
Allen
Sent: Thursday, 20 August 2009 6:57 AM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Amyloid st
Hi,
What stain works for amyloid on frozen muscle bx tissue? I did the a
Congo Red stain without much success.
Thanks
Sharon
sal...@hsc.mb.ca
This email and/or any documents in this transmission is intended for the
addressee(s) only and may contain legally privileged or confidential
information.
Hello fellow Histonetters,
I was wondering if anyone is running Biocare's Amyloid A or Amyloid P
antibodies on Ventana's Benchmark. If so, are you using the concentrated or
predilute antibody?
Also, are you running these alone or with a Congo Red stain (as in a panel)?
Any information is great
Does anyone have any Amyloid tissue they are willing to share?? Thanks,
Cheri
Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne.
402 738 5052
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you
are not the addressee intende
Hi Everyone~
We are having a hard time finding Amyloid controls. Can anyone
recommend a company that has good controls?
Thanks~
Beth
Beth Delescavage, BS, HTL (ASCP) QIHC
DISCLAIMER:
This message is intended for the sole use of the addressee, and may contain
information that is privileged
ist
Molecular Aging & Development Lab
Boston University Medical Campus
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, September 16, 2008 11:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid
We have been
Dorothy:
We does Highman's method (Bancroft&Stevens, 1977 p.164)
Both 8 and 10 microns give good results.
Maxim Peshkov
Russia,
Taganrog.
mailto:[EMAIL PROTECTED]
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http://li
No, but you should ask your pathologist where that "standard" comes from. S/he
should know since s/he brought the issue up.
René J.
--- On Tue, 9/16/08, Webb, Dorothy L <[EMAIL PROTECTED]> wrote:
From: Webb, Dorothy L <[EMAIL PROTECTED]>
Subject: [Histonet
No, but you should ask your pathologist where that "standard" comes from. S/he
should know since s/he brought the issue up.
René J.
--- On Tue, 9/16/08, Webb, Dorothy L <[EMAIL PROTECTED]> wrote:
From: Webb, Dorothy L <[EMAIL PROTECTED]>
Subject: [Histonet
We have been cutting our tissue for amyloid staining @ 8 microns. One
of my pathologists heard that 10 microns is now standard and to use a
negative and weakly positive control. Does anyone have any new
information in this area? Thanks ahead of time!
Dorothy Webb, HT (ASCP)
Histology Technical
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