...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Rui TAHARA
Gesendet: Freitag, 23. August 2013 09:49
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Bone/cartilage/epithelial tissue stain
Hi,
Would anyone suggest me what staining is best to color differentiate
A HE will allow you to identify those three tissue types, in normal tissues,
of most organisms ( certainly
in avian embryos, imho), IF you know your Histology.
There is no stain/demonstration technique that will substitute for this
knowledge, imho.
Sure, a special stain will help to confirm
Hi,
Would anyone suggest me what staining is
best to color differentiate between cartilage and bone and epithelial tissues
in avian embryos?
I have been trying Mallory Trichrome for
embryos but recently I was suggested that Mallory Trichrome stains cartilage
differently
in embryos compared
At one time I did a lot of work on cartilage and growth plate
and used Toluidine Blue and Fast Green. T-blue (buffered appropriately)
stains the
proteoglycan a lovely metachromatic blue and the bone and most
everything else
is green. The nuclei of cells are also green since we used no nuclear
