[Histonet] C4d

Hello, Does anyone in here run C4d (SP91) on the Benchmark Ultras and would be willing to share your protocol? I am having a really hard time getting this stain to where the pathologists want it. Thank you Emily Horst Histology Supervisor University of Cincinnati Medical Center

[Histonet] C4d IHC in myocardial biopsy

Are any of you an expert with C4d IHC in myocardial biopsy tissue? We had a specimen this week that showed a pattern of immunoreactivity that I am not familiar with and I need to find out if this represents a specific disease process. Thank you. Richard Richard W. Cartun, MS, PhD Director,

AW: [Histonet] C4d IF on FFPE kidney

-Ursprüngliche Nachricht- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Mittwoch, 01. Februar 2012 21:21 An: gu.l...@gmx.at Betreff: Re: [Histonet] C4d IF on FFPE kidney Hi Gudrun: Is there reason why you want to use IF and not immunoperoxidase? Richard Richard W. Cartun, MS, PhD

Re: AW: [Histonet] C4d IF on FFPE kidney

2012 21:21 An: gu.l...@gmx.at Betreff: Re: [Histonet] C4d IF on FFPE kidney Hi Gudrun: Is there reason why you want to use IF and not immunoperoxidase? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] C4d IF on FFPE kidney

Hi! Can someone provide me a immunofluorescence protocol for C4d on formalin fixed human paraffin sections? thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] C4d immunofluorescent positive controls

We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest

[Histonet] C4d

Hi Everyone, Does anyone know of a FITC C4d that is ASR or IVD? All I have been able to find is RUO. Also, is anyone using something different to show rejection in transplant kidneys? Thank you, Lacie Lacie Algeo, HTL, MP(ASCP) Histology Supervisor Providence Sacred Heart Medical Center

[Histonet] C4d nuclear staining?

Has anyone ever noticed any nuclear staining with C4d in a renal biopsy? The control stains great, but the patient's tissue exhibits some nuclear staining in what look to be inflammatory cells. There has been a report of granular and some nuclear staining in lung transplants on structures

[Histonet] C4d stain for IF

Patrick, or anyone else using the alpco product...can you give some details/feedback on how this anti human C4d (FITC-Conjugated) is working for you, and whether you were able to replace two steps with one for C4d IF staining? I checked that website, and the pdf on this product reads: The

[Histonet] C4d on paraffin sections

I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much!

AW: [Histonet] C4D Antibody

] Im Auftrag von kk...@bidmc.harvard.edu Gesendet: Freitag, 21. August 2009 19:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4D Antibody Just wondering if anyone out there routinely works with C4d. I was wondering what you use for retrieval and the Type of detection kit you use

[Histonet] C4d

Thx Everyone for your input on C4D..I'm running it right now. Trying new things and special thx to Sally Drew. Kwadwo kwaa BIDMC Pathology Department 617-667-5769 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

AW: [Histonet] C4d Antibody

Francisco Romero Gesendet: Dienstag, 24. März 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now

Re: [Histonet] C4d Antibody

We get our from Alpco. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced!

AW: [Histonet] C4d Antibody

: Dienstag, 24. März 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal

[Histonet] C4d Antibody

Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations

RE: [Histonet] C4d question (not CD4)

Cartun; Histonet Subject: RE: [Histonet] C4d question (not CD4) Biocare has an IVD predilute that is very good. (PM153AA) We use it on our Benchmark XT. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL

[Histonet] C4d question (not CD4)

We have been using an RUO-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years. We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is