Hello,
Does anyone in here run C4d (SP91) on the Benchmark Ultras and would be
willing to share your protocol? I am having a really hard time getting this
stain to where the pathologists want it.
Thank you
Emily Horst
Histology Supervisor
University of Cincinnati Medical Center
Are any of you an expert with C4d IHC in myocardial biopsy tissue? We had a
specimen this week that showed a pattern of immunoreactivity that I am not
familiar with and I need to find out if this represents a specific disease
process. Thank you.
Richard
Richard W. Cartun, MS, PhD
Director,
-Ursprüngliche Nachricht-
Von: Richard Cartun [mailto:rcar...@harthosp.org]
Gesendet: Mittwoch, 01. Februar 2012 21:21
An: gu.l...@gmx.at
Betreff: Re: [Histonet] C4d IF on FFPE kidney
Hi Gudrun:
Is there reason why you want to use IF and not immunoperoxidase?
Richard
Richard W. Cartun, MS, PhD
2012 21:21
An: gu.l...@gmx.at
Betreff: Re: [Histonet] C4d IF on FFPE kidney
Hi Gudrun:
Is there reason why you want to use IF and not immunoperoxidase?
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic
Hi!
Can someone provide me a immunofluorescence protocol for C4d on formalin
fixed human paraffin sections?
thanks in advance
Gudrun Lang
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We are interested in finding out what type of positive control tissue everyone
is using when performing C4d immunofluorescence on heart biopsies. Thanks in
advance for any and all responses.
Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest
Hi Everyone,
Does anyone know of a FITC C4d that is ASR or IVD? All I have been able
to find is RUO. Also, is anyone using something different to show
rejection in transplant kidneys?
Thank you,
Lacie
Lacie Algeo, HTL, MP(ASCP)
Histology Supervisor
Providence Sacred Heart Medical Center
Has anyone ever noticed any nuclear staining with C4d in a renal biopsy? The
control stains great, but the patient's tissue exhibits some nuclear staining
in what look to be inflammatory cells. There has been a report of granular and
some nuclear staining in lung transplants on structures
Patrick, or anyone else using the alpco product...can you give some
details/feedback on how this anti human C4d (FITC-Conjugated) is working for
you, and whether you were able to replace two steps with one for C4d IF
staining? I checked that website, and the pdf on this product reads:
The
I need some advice. We have been trying to do C4d on paraffin sections
without much success. Does anyone have a good protocol we could try?
We use the C4d from Quidel for our frozen sections and ideally I would
like a procedure that we can use with our Bond Max, but I may be hoping
for too much!
] Im Auftrag von
kk...@bidmc.harvard.edu
Gesendet: Freitag, 21. August 2009 19:39
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] C4D Antibody
Just wondering if anyone out there routinely works with
C4d. I was wondering what you use for retrieval and the
Type of detection kit you use
Thx Everyone for your input on C4D..I'm running it right now.
Trying new things and special thx to Sally Drew.
Kwadwo kwaa
BIDMC
Pathology Department
617-667-5769
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Francisco Romero
Gesendet: Dienstag, 24. März 2009 05:01
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] C4d Antibody
Dear People from Histonet
Could you share your experience with C4d Antibody ?
I couldn't get any results with the mouse monoclonal from Quidel.
I will try now
We get our from Alpco.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!
: Dienstag, 24. März 2009 05:01
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] C4d Antibody
Dear People from Histonet
Could you share your experience with C4d Antibody ?
I couldn't get any results with the mouse monoclonal from Quidel.
I will try now the rabbit polyclonal
Dear People from Histonet
Could you share your experience with C4d Antibody ?
I couldn't get any results with the mouse monoclonal from Quidel.
I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe.
I work in a Public Hospital with many kidney transplantations
Cartun; Histonet
Subject: RE: [Histonet] C4d question (not CD4)
Biocare has an IVD predilute that is very good. (PM153AA) We use it on
our Benchmark XT.
Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
Chico, CA
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL
We have been using an RUO-labeled anti-C4d antibody on certain renal
transplant cases for the immunohistochemical detection of humoral rejection for
the past several years. We have now been asked by our Transplant team to do
this test routinely on all transplant kidney biopsies (which is
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