Hi fellow Histo people!
I am setting up a new histology lab and I am needing to find inventive ways
to obtain controls for IHC and Special stains. I have lots of normal tissue
but I am in need of melanoma, P16 positive, fungus, positive p63, and
positive p504. If anyone would be interested in
7 PM
To: 'Histonet (histonet@lists.utsouthwestern.edu)'
Subject: [Histonet] Controls
STOP, THINK, READ. This is an external email. Exercise extra caution responding
to it, opening attachments and following links.
Good afternoon!
Could any of you tell me off hand how old patient tissue has to be before you
Good afternoon!
Could any of you tell me off hand how old patient tissue has to be before you
can use it as a control? My lab has only been operating since February. We run
quite a few Alcian Blue PAS stains a week, and my last control is getting
sparse. I have some beautiful patient tissue
Hello Garrey,
Curious myself, CAP contact info seems to be greyed out on website unless I
officially log in and for now my concerns are with the Washington State Science
and Engineering Fair for K-12 and golf game.
(1) There are at least two phrases in the ANP.21450 which could be parsed
Subject: Re: [Histonet] Controls
I am in need of both a gram and fungal control and will have to try the slim
Jim, hamburger and hotdog tricks. Thanks. If they work I will post
A link to pictures.
Sent from my iPhone
On Mar 5, 2015, at 11:58 AM, Ronda Mire rm...@cvpath.org wrote
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ronda Mire
Sent: Thursday, March 05, 2015 8:59 AM
To: Baker, Michael
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Controls
Slim Jim will work as a control for gram stain. Can you believe people eat
this crap?
On Mar 5, 2015
-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Wednesday, March 04, 2015 1:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls:
Off the wall question, I have been told that slim jims (pepperoni stick) at the
gas station can be processed and used as good gram controls
-requ...@lists.utsouthwestern.edu
wrote:
--
Message: 9
Date: Wed, 4 Mar 2015 14:04:52 -0700
From: Jb craiga...@gmail.com
Subject: [Histonet] Controls:
To: Histonet@lists.utsouthwestern.edu
Message-ID: b98f49d1-de91-4675-b53b-a34cfcc43...@gmail.com
Content-Type
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
There was actually an article written in The Journal of Histotechnology a
few years back on this and it was, I f I recall, using a hot dog to create
the gram control. Might be in the archives on nsh.org.
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
On Mar 5, 2015, at 10:51 AM, histonet-requ...@lists.utsouthwestern.edu wrote:
--
Message: 9
Date: Wed, 4 Mar 2015 14:04:52 -0700
From: Jb craiga...@gmail.com
Subject: [Histonet] Controls:
To: Histonet@lists.utsouthwestern.edu
Message-ID: b98f49d1-de91-4675
Sent: Thursday, March 05, 2015 9:21 AM
To: Jb; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
We have successfully used hamburger meat to make Gram controls. As far as GMS
controls go, we ran across a post from someone smearing cream cheese onto lung
tissue and letting it sit
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Wednesday, March 04, 2015 1:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls:
Off the wall question, I have been told that slim jims (pepperoni
, March 05, 2015 7:21 AM
To: Jb; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
We have successfully used hamburger meat to make Gram controls. As far as GMS
controls go, we ran across a post from someone smearing cream cheese onto lung
tissue and letting it sit for a couple
[mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Thursday, March 05, 2015 7:21 AM
To: Jb; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
We have successfully used hamburger meat to make Gram controls. As far as
GMS controls go, we ran
...@cvpath.org
Cc: histonet@lists.utsouthwestern.edu, Baker, Michael
michael.ba...@cchmc.org
Date: 03/05/2015 11:10
Subject: Re: [Histonet] Controls
I am in need of both a gram and fungal control and will have to try the slim
Jim, hamburger and hotdog tricks. Thanks. If they work I will post
Of James Watson
Sent: Thursday, March 05, 2015 1:01 PM
To: 'Jb'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:
Years ago (70's and 80's) Slim Jims were used as Gram pos neg controls in all
the labs at AFIP.
James Watson HT ASCP
GNF Genomics Institute of the Novartis
Off the wall question, I have been told that slim jims (pepperoni stick) at the
gas station can be processed and used as good gram controls. Has anyone done
this and do they work for GMS also?
Thank you,
Sent from my iPhone
___
Histonet mailing list
Help! Running low on CMV and AFB positive control blocks. Looking into buying
precut slides but the price is steep. Any suggestion ? Which company has the
best price.
The information in
Is a candid control one that's taken when the fungus doesn't know you're
sampling it?
Sincerely?
Jay A Lundgren,
M.S., (HTL) ASCP
On Fri, Jan 10, 2014 at 11:58 AM, Webb, Dorothy L
Does anyone out in histoland have a Candid control for fungus that you could
spare? We are very much in need and would appreciate the help and see what we
could possibly trade for.
Much thanks and have a great week-end all!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
What are people using to control direct IF?
We use frozen tonsil for immunoglobulins and light chains, but what about
fibrinogen, albumin, C1q, C3c and C4c?
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
We are in need of Aspergillus and Amyloid controls. Does anyone on the
histonet have any suggestions, or overabundance that they could share with us?
Thanks,
Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport,
TN 423 224-5793 fax 423 224-5349
It is the most effective and economical way to run your IHC's.
We have been doing it that way for as long as I have been in histology.
Thanks,
Tim
- Original Message -
From: histonet-requ...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, July 19, 2011 6:04
Of
Rathborne, Toni
Sent: Wednesday, July 20, 2011 8:19 AM
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks
for a case, or just on one
: Richard Cartun [mailto:rcar...@harthosp.org]
Sent: Tuesday, July 19, 2011 6:40 PM
To: histonet@lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch
controls. Many
In our Lab we put a control on every slide. We keep multiple controls cut
and stored in refrigerator. Most are multi- tissue controls. We use the
Ventana detection kits and it is not cost efficient to use 2 uses of the
detection kit per antibody. Of course there are times that this will not
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, July 20, 2011 9:19 AM
To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient
Hi Toni,
We put the control on the same slide and after it's stained and coverslipped
we draw a line to seperate the control and patient tissue with each side of
the line having either a C or P written on it.
Mark
On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni
, 2011 2:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls with patient specimen on same slide
Hi,
I'm interested in knowing how many of you are performing ihc with the
control tissue and the patient tissue on the same slide. I have seen
slides available which have designated
...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Tuesday, July 19, 2011 2:43 PM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Controls with patient specimen on same slide
Hi Toni,
We put the control on the same slide and after it's stained and coverslipped
we
We do not put our positive control tissue on the test slide; we run batch
controls. Many of the unstained slides (breast, GI, and prostate biopsies)
that we use for IHC testing are cut in our Histology Laboratory as part of a
part-type slide protocol. For example, we cut 7 slides, 2 sections
] On Behalf Of Richard Cartun
Sent: Tuesday, July 19, 2011 18:40
To: histonet@lists.utsouthwestern.edu; Toni Rathborne
Subject: Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch
controls. Many of the unstained
This response is late due to the overwhelming time commitments of NCAA
Basketball Tournaments.
The only multi-tissue control we use is for HER2 protein overexpression. In
my opinion, multi-tissue controls are completely unnecessary for every-day
use for the majority of IHC tests that we do.
Hello everyone,
I have an issue that I would like some help with. We are currently running
SMM-HC and E-Cad on Breast cores and specimens from a derm lab. With the
regulations being so strict regarding the processing times of breast
specimens, how are you all dealing with that when it comes
Hello,
It seems we go back and forth between multi-tissue control blocks and
single tissue control blocks when it comes to immunostaining.
In the past we used single blocks and still continue to use some for the
IPs that are rarely used. Then, switched to creating multi-tissue
blocks for
Does anyone out there in Histoland have a control block for MDM2 for
liposarcoma that they would be willing to share? We don't get very many
liposarcomas so control blocks are a problem. Any help is greatly appreciated.
Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
I second that. Marsha is the loveliest person I know.
- Original Message -
From: Denise Piontek dbpion...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, April 08, 2009 8:49 PM
Subject: RE: [Histonet] Controls needed!
Newcomer Supply has been a reliable control
...@primecare.org]
Sent: Tuesday, April 07, 2009 3:06 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Controls needed!
We are looking for H. Pylori control tissue and also GMS/fungus control
tissue. Is there anyone out there that might have extra to share? We
have
good GRAM control blocks
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Hofecker, Jennifer L
Sent: 08 April 2009 13:14
To: Knutson, Deanne; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls needed!
Hi Deanne,
Have you checked with the NSH Control Tissue Bank
We are looking for H. Pylori control tissue and also GMS/fungus control
tissue. Is there anyone out there that might have extra to share? We have
good GRAM control blocks that we would be happy to exchange. Please let me
know if you can help us out. Thank you!
Deanne Knutson
Anatomic
41 matches
Mail list logo
