Hi Eric
Thanks for your input regarding the Citrate buffer pH2.0. Yep this pH came
as a surprise to me too. The data sheet from the company specifies 0.01M
citrate at pH2.0 and a publication which used the same antibody also
indicates a "modified citrate buffer" but doesn't give the exact p. I wil
-boun...@lists.utsouthwestern.edu] On Behalf Of Neil Macintyre
Sent: Tuesday, April 17, 2012 10:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Citrate Buffer pH2.0
Hi
Can anyone help me with a formula for 0.01M citrate buffer pH2.0 for antigen
retrieval as this pH has been recommend
Why do you want the pH so low? Citrate buffers are most effective in the
range of pH 3.0-6.0.
I would check with the company and make sure that the pH is supposed to be
2.0, and ask them for a formula to achieve such a solution. It probably
won't have much buffering capacity at pH 2.0.
There is
Hi
Can anyone help me with a formula for 0.01M citrate buffer pH2.0 for antigen
retrieval as this pH has been recommended by the company supplying AM3K
macrophage antibody and I can't find this in any of the usual IHC buffer
protocols.
Thanks
Neil MacIntyre CSci FIBMS
Laboratory Manager
Veterin
