[Histonet] Congo Red filters

Fran - You can use Whatman #1 filter paper but it is a lot cheaper and just as good to use 2 coffee filters. They are the right size for filtering stains. Cheryl Crowder ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Congo Red

Carl Hobbs FIBMS, Histology and Imaging Manager at Kings College in London replies: >>That's a good point, Bob (mouse amyloid controls for human sections). The amyloid produced by the the various mouse models (TASTPM, TGs) is human amyloid... as you know. I suspect that it wouldn't be accepted as

Re: [Histonet] Congo Red- Glass wool

26, 2018 12:28 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Congo Red- Glass wool Can anyone tell me what kind and/or size glass wool do you use to for filtering the Working Congo Red solution right before use? From what I've read, it recommends not to use regular filter paper. Thank yo

[Histonet] Congo Red- Glass wool

Can anyone tell me what kind and/or size glass wool do you use to for filtering the Working Congo Red solution right before use? From what I've read, it recommends not to use regular filter paper. Thank you so much, Fran Pearsall, ASCP ___ Histonet

Re: [Histonet] Congo Red

That's a good point, Bob (mouse Amyloid controls for human sections) The amyloid produced by these various mouse models (TASTPM, TGs) is human amyloidas you know. I suspect that it wouldn't be accepted as a positive control ( I don't know) because it isn't pathologically produced It is

Re: [Histonet] Congo Red

You will need a polarizing filter. The size is just the necessary to cover your light source. René On Friday, March 23, 2018 10:27 AM, Frances Pearsall via Histonet wrote: Can anyone tell me what kind (or size)  glass filter to use to perform

[Histonet] Congo Red

Can anyone tell me what kind (or size) glass filter to use to perform Puchtlers Congo Red? And are the filters re-useable? Thank you, Fran Pearsall ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Congo red and LED illumination problem?

Something new to me... A pathologist said he is getting reports that LED illumination on newer microscopes is leading to faint or even false negatives for congo red amyloid birefringence due to the difference in spectrum between LED and tungsten filament lamps. Has anyone else noticed this?

Re: [Histonet] Congo Red Controls

e: 11/14/2016 12:26 PM Subject:[Histonet] Congo Red Controls Good Afternoon Histonet, How does everyone handle their Congo Red Controls; thickness, room temp or refridgerator, how long do they last once cut, keep them baked or unbaked and anything else I did not list. Thank you ve

[Histonet] Congo Red Controls

Good Afternoon Histonet, How does everyone handle their Congo Red Controls; thickness, room temp or refridgerator, how long do they last once cut, keep them baked or unbaked and anything else I did not list. Thank you very much. Irene Irene Myles, HT (ASCP) Senior Histology Technician

Re: [Histonet] congo red - decolor

boratories > > 205 Bluff Street, Suite 1 > > Dubuque, IA 52001 > > Ph. 563-690-4142 > > Check us out at www.uclaccess.com > > > > > > > > > > *From:* Jay Lundgren [mailto:jaylundg...@gmail.com] > *Sent:* Thursday, March 17, 2016 13:10 PM >

Re: [Histonet] congo red - decolor

Are you sure you don't mean Oil Red O stain for fat? Just checking. Congo Red is for amyloid. Destain Congo Red by soaking in 100% Propylene Glycol, warmth and stirring would speed it up. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Mar 17, 2016 at 12:41 PM, Nancy

[Histonet] congo red - decolor

Happy St. Pat's Day! Any thoughts on how to decolorize Congo Red stains that were done on fat pad slides? We appreciate any insight you might offer:) Nancy United Clinical Labs Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the

[Histonet] Congo Red problems

Hi All: Lately, I have been having problems with my Congo Red staining too lightly. The Bielschowsky silver stain shows lots of amyloid in the cerebral blood vessels and the cores of the senile plaques. However, the Congo Red stain is pretty faint, although you can still see it. I have just

Re: [Histonet] Congo Red problems

Why don´t you use the classic Highman´s Congo red hidroalcoholic solution? (0,5% in 80% ethanol). Best luck, Carlos. *** REPLY SEPARATOR *** On 26/11/2014 at 20:48 Wheelock, Timothy R. wrote: Hi All: Lately, I have been having problems with my Congo Red staining too lightly.

[Histonet] Congo red stain

We have been experiencing major difficulty with our Congo Red stain. We used to do it on the Artisan, but they changed raw products which has altered the stain and is not acceptable by our hematopathologists. Now we are back to manual and cannot seem to get it consistently acceptable. I

[Histonet] congo red

Hi to all- What is your process for pretreating congo red smears? Are you fixing them? We are running the stain on the Dako Artisan staining system. Thanks Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s)

Re: [Histonet] congo red

Yes, smears should be fixed. I used NBF. René J. From: Nancy Schmitt nancy_schm...@pa-ucl.com To: Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Sunday, February 3, 2013 7:48 AM Subject: [Histonet] congo red Hi to all- What is your process for pretreating

Re: [Histonet] Congo red

The opinions expressed are mine, and do not reflect those of my employer. -Original Message- From: Bryan Llewellyn Sent: Thursday, March 01, 2012 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Congo red Bennhold's method is not the easiest to give good results

[Histonet] Congo red

Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol

Re: [Histonet] Congo red

Bennhold's method is not the easiest to give good results. Try Highmans's method (http://stainsfile.info/StainsFile/stain/amyloid/congohighman.htm). It is much easier to control. Remember that congo red is not an intensely coloured dye and the results are often pale. If your pathologists

Re: [Histonet] Congo red

If it's taking you a week, you're doing it wrong. Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) __**_ Histonet mailing list

[Histonet] Congo Red for Amyloid

Need help with Congo Red for Amyloid problem.  We have been using automated staining with Dako's Artisan and recently tried manual procedure with fresh reagents from Polyscientific kit with little to no staining on two commercially prepared controls and two different patients who have been sent

Re: [Histonet] Congo Red control slides

- Original Message - From: mesruh turkekul turke...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 30, 2011 2:45 PM Subject: [Histonet] Congo Red control slides Hi, I would like to know if anybody is purchasing positive control tissue slides for congo red staining. Can you

RE: [Histonet] Congo Red control slides

American Mastertech, Cat. #CSA0425P -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mesruh turkekul Sent: Tuesday, August 30, 2011 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Congo Red

[Histonet] Congo Red control slides

Hi, I would like to know if anybody is purchasing positive control tissue slides for congo red staining. Can you suggest any vendor and catalog number? Thanks, Mesru Turkekul mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Congo Red Controls

Hi,A pathologist told me to try medullary thyroid carcinoma cases for Congo Red. Cheaper than purchasing if positive.Good luck! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] congo red staining method for abdominal fat aspirates

dear colleagues, I have a problem with congo red staining of abdominal fat aspiration slides.i perform the same way as for slides prepared from paraffin-embedded blocks, but the results are  poor.     thank you in advance for your help.     sincerely, sabeti 

[Histonet] congo red

TGIF!! We are having a bit of a problem with Congo Red control coming off; we keep the block refrigerated and reseal after each use. The patient specimen does not seem to be an issue and both are cut at 6. We do not cut control slides ahead of time. Any thoughts would be appreciated. These