You don't need to decolorize. Remove the coverslip, rehydrate to water (the
eosin will come out in the alcohol), and then begin your IHC procedure.
Hopefully, the tissue is mounted on an adhesive or charged slide. If not, you
may have to cut back your antigen retrieval. Also, you may want to
al & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Paula
Lucas
Sent: Monday, October 13, 2008 2:38 PM
To: [EMAIL PROTECTED]
Subject: [Histonet] Decolorize for IHC
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Paula
Lucas
Sent: Monday, October 13, 2008 2:38 PM
To: [EMAIL PROTECTED]
Subject: [Histonet] Decolorize for IHC
Hello,
I nee
Hello,
I need to decolorize an H&E stained section for Cytokeratin 20 and I would like
to know the proper way of decolorizing for IHC. Is it even possible to get a
CK 20 positive stain after doing so?
Thanks,
Paula Lucas HT
Lab Manager
Bio-Path Medical Group
__
