We did use freeze spray in the cryostat years ago. Then one fateful day we all
had to go on protocol for exposure to TB because a resident sprayed a lung with
the freeze spray and there you go. There is a turn around time for frozen
sections we are all working with, HOWEVER it is not worth
I make my tech’s wear PPE’s, goggles/masks, gloves and N95 masks for potential
TB and regular masks for normal tissue.
Akemi Allison
Sent from my iPhone
> On Sep 25, 2019, at 1:00 PM, Charles Riley via Histonet
> wrote:
>
> I have to agree with Terri. I also believe that CAP is going to be
I have to agree with Terri. I also believe that CAP is going to be putting
in regulations forbidding the use of freeze sprays in cryostats soon (at
least I think I heard a rumor about this).
Anyway another useful item is liquid nitrogen if you really need a quick
freeze. Dip a brush into the
My question is why would you ever want to use an aerosol in the cryostat,
taking a chance on aerosolizing some nasty bug?
Is the 30 seconds you save in using freezing spray worth the exposure to TB or
goodness-knows-what?
We use a simple metal heat extractor (comes standard in our Leicas) and
