Health-MDH; (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] frozen section problem
Hi Dorianne,
You need to freeze your tissue faster. Ideally, isopentane placed in a
metal cup, that is that is then frozen in a dewar of liquid nitrogen, works
best. The isopentane, o
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-Original Message-
From: Bonello Dorianne M at Health-MDH via Histonet
Sent: Friday, July 16, 2021 11:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] frozen section problem
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From: Bonello Dorianne M at Health-MDH via Histonet
Sent: July 16, 2021 11:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] frozen section problem
Dear all,
We are experiencing freezing artifacts on our frozen sections. Basically
Dorianne Bonello, Allied Health Practitioner (MLS), Histology Laboratory -
Pathology Health-Mater Dei Hospital, on the island of Malta asks:
> >>We are experiencing freezing artifacts on our frozen sections.
> Basically, we are seeing cavity-like structures under the microscope,
> mostly
Dear all,
We are experiencing freezing artifacts on our frozen sections. Basically, we
are seeing cavity-like structures under the microscope, mostly elongated,
especially when it's a frozen section on brain tissue. This is most probably
happening due to ice crystal formation. We're not using
