RE: [Histonet] Good Perfusion..."Sacrifice" vs "anesthesia"

2009-07-01 Thread JR R
com To: chana.de.w...@gmail.com; jf...@gladstone.ucsf.edu CC: histonet@lists.utsouthwestern.edu; rosenfeld...@hotmail.com Subject: Re: Re: [Histonet] Good Perfusion... I think many, many labs perfuse animals under deep anesthesia. Notice the word "DEEP". It is hard to judge whether the animal

RE: Re: [Histonet] Good Perfusion...

2009-07-01 Thread gayle callis
estern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TF Sent: Tuesday, June 30, 2009 11:46 PM To: Chana de Wolf; jfish Cc: histonet; JR R Subject: Re: Re: [Histonet] Good Perfusion... I think many, many labs perfuse animals under deep anesthesia. Notice the word "DEEP&

Re: Re: [Histonet] Good Perfusion...

2009-07-01 Thread Merced M Leiker
Hey everyone...just thought I'd add an "official" word from the authorities in this debate...as I'd been taught or somehow led to believe otherwise: :-) Forwarded Message Date: Wednesday, July 01, 2009 11:19 AM -0400 From: IACUC To: Merced M Leiker Subject: RE: gener

Re: Re: [Histonet] Good Perfusion...

2009-06-30 Thread TF
7-01 03:42:30 收件人: jfish 抄送: histonet; JR R 主题: Re: [Histonet] Good Perfusion... Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic c

Re: Re: [Histonet] Good Perfusion...

2009-06-30 Thread TF
let me say like this during the overdose injection, look into the animals' eyes. take their soul away. then the pre-mortem perfusion is humanized. 2009-07-01 TF 发件人: Chana de Wolf 发送时间: 2009-07-01 09:30:01 收件人: Ingles Claire 抄送: histonet 主题: Re: [Histonet] Good Perf

Re: [Histonet] Good Perfusion...

2009-06-30 Thread Chana de Wolf
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Chana de Wolf > Sent: Tue 6/30/2009 2:37 PM > To: jf...@gladstone.ucsf.edu > Cc: histonet@lists.utsouthwestern.edu; JR R > Subject: Re: [Histonet] Good Perfusion... > > > > Histonetters, > > Perfusion under deep anesthesia

RE: [Histonet] Good Perfusion...

2009-06-30 Thread Ingles Claire
f Chana de Wolf Sent: Tue 6/30/2009 2:37 PM To: jf...@gladstone.ucsf.edu Cc: histonet@lists.utsouthwestern.edu; JR R Subject: Re: [Histonet] Good Perfusion... Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is n

[Histonet] Good Perfusion--I stand corrected.

2009-06-30 Thread JR R
I stand correct on the perfusion under anesthesia issue. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Tue, 30 Jun 2009 12:37:20 -0700 Subject: Re: [Histonet] Good Perfusion... From: chana.de.w...@gmail.com To: jf...@gladstone.ucsf.edu CC: rosenfeld

Re: [Histonet] Good Perfusion...

2009-06-30 Thread Chana de Wolf
t; The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R > Sent: Tuesday, June 30, 2009 10:49 AM >

RE: [Histonet] Good Perfusion...

2009-06-30 Thread Jo Dee Fish
ssage- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Tuesday, June 30, 2009 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Perfusion... No, I wouldn't nick the atrium. Instead, cut the femoral

[Histonet] Good Perfusion...

2009-06-30 Thread JR R
No, I wouldn't nick the atrium. Instead, cut the femoral artery at the groin. And of course, you cannot perfuse a living, anesthetized animal. That would be unethical and also illegal. Jerry Ricks Research Scientist University of Washington Department of Pathology ___