Hi all, Since the key to the question lies in Optimal Fixation, the answer to
this question is A, and it is taken right out of the Histotechnology
self-instructional text 2nd edition pg 98. (See note)
Under Frozen Sections
I've included a procedure I have used successfully in the past. It
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy
Wenk
Sent: Thursday, October 03, 2013 9:22 AM
To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...
I agree, there is probably more
Hi,
Please clarify why this answer to the HistoDeck study question is a) and not
b).
Here is the question:
'Frozen section slides cut from fresh, unfixed tissue specimens are optimally
fixed in which of the following solutions?
a) 37%-40% formaldehyde
b) Cold acetone
c)
, Sheryl
Sent: Thursday, October 03, 2013 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HT HistoDeck question...
Hi,
Please clarify why this answer to the HistoDeck study question is a) and
not b).
Here is the question:
'Frozen section slides cut from fresh, unfixed tissue
...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk
Sent: Thursday, October 03, 2013 8:16 AM
To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...
Personally, I think it's a is a wrong answer, and that you are correct
that b is a better
[mailto:histonet-
boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk
Sent: Thursday, October 03, 2013 8:16 AM
To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...
Personally, I think it's a is a wrong answer, and that you
: [Histonet] HT HistoDeck question...
We fix HE's in 95% and our IHC protocol is acetone/alcohol fixation.
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
; Stephenson, Sheryl ;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HT HistoDeck question...
For frozen cut sections, would the fixation also depend on what you plan on
doing with it. For example, HE, Special Stain or IHC? Please correct if I
am wrong. I think that is a trick question
Sent: Thursday, October 03, 2013 9:22 AM
To: Watson, Linda; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...
I agree, there is probably more than one correct answer to this question,
depending upon whether you are planning on doing stains
I'd go with A, but it really depends on what you are going to do with the
sections after fixation.
In the protocol for Oil Red O in Freida's second edition (that I use almost
daily combined with some steps from PolyScientific's ORO protocol), step #1
says to fix in 40% formaldehyde. Doesn't
) algra...@email.arizona.edu
To:
Cc: HISTONET histonet@lists.utsouthwestern.edu
Date: 10/03/2013 08:33 AM
Subject:Re: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu
I'd go with A, but it really depends on what you are going to do
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Thursday, October 03, 2013 2:04 PM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] HT HistoDeck question...
We also use this oil red O method and use the 40
: Thursday, October 03, 2013 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HT HistoDeck question...
Hi,
Please clarify why this answer to the HistoDeck study question is a) and not
b).
Here is the question:
'Frozen section slides cut from fresh, unfixed tissue
...@lists.utsouthwestern.edu] Im Auftrag von Rathborne,
Toni
Gesendet: Donnerstag, 03. Oktober 2013 20:34
An: 'Jennifer MacDonald'; Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Betreff: RE: [Histonet] HT HistoDeck question...
For those of you who use the 40
@lists.utsouthwestern.edu,
histonet-boun...@lists.utsouthwestern.edu
histonet-boun...@lists.utsouthwestern.edu
Date: 10/03/2013 11:42 AM
Subject:RE: [Histonet] HT HistoDeck question...
Sent by:histonet-boun...@lists.utsouthwestern.edu
For those of you who use the 40% formaldehyde
15 matches
Mail list logo
