Nancy,
You used the word “blot” in your reply to Charles, and while I can’t be
certain that you did not mean the same...I would like say in the interest
of clarity, that it is safer to carefully “wick” the water away from under
each section prior to baking. Blotting (like we might do after a Gram
To: Histo List
Subject: [Histonet] IHC troubleshooting
EXTERNAL email from Outside HHC! Do NOT open attachments or click links from
unknown senders.
Our pathologists are complaining that chunks of the dermis are missing from IHC
slides yet the entire section is present prior to staining.
Does anyone
onet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 26, 2020 9:44 AM
To: Histo List
Subject: [Histonet] IHC troubleshooting
EXTERNAL email from Outside HHC! Do NOT open attachments or click links from
unknown senders.
Our pathologists are complaining that chunks of the dermis are mis
Hi Charles,
It adhesion problems can arise from several sources, some have already been
mentioned. Here are two that I have experienced:
1) Bad slides. Either a manufacturing defect such that the positive charge
is insufficient or the slides were somehow compromised after opening them
on the bench
In my experience;
If the the specimen is under-fixed or under-processed, the section will be
sloughed off during IHC staining.
I find proper fixation time and drying the slides at room temperature (before
baking them in oven) optimal.
Andy
Abdulbasit Andijany (Andy)
AMLS, BBMS, MSc, CT
Do you float and pick up your sections from a water bath of distilled water
with no adhesive added? If not, doing that will help.
John
On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet
wrote:
> Our pathologists are complaining that chunks of the dermis are missing from
> IHC slides
Our pathologists are complaining that chunks of the dermis are missing from
IHC slides yet the entire section is present prior to staining.
Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process? We use the Leica Bond stainers.
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