Hi all,
Looking for pros and cons on immunostainers for a small volume laboratory.
Thanks in advance,
Valerie
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Ted Pella has some options. We use the Immunostain Moisture Chambers
#21049, but there are others.
http://www.tedpella.com/glasswar_html/slidedsh.htm#21049
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S and then start
your IHC procedure.
Robert Schoonhoven, HT/HTL (ASCP)
From: Allyse Mazzarelli
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 13, 2013 1:19 PM
Subject: [Histonet] Immuno Bed Plastic Question
Hi histonetters!
I have posted on
I think that you should bring this issue to the provider. For sure you will get
a better answer than bringing this issue to HistoNet.
René J.
From: Allyse Mazzarelli
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 13, 2013 1:19 PM
Subject: [Histonet] Immuno Bed Plastic Question
Hi histonetters!
I have posted on this forum a while ago asking for opinions regardnig new
types of plastics that work well with IHC. It was brought to my attention
that "Immuno-Bed" (Polysciences) works rather well. I recently purchased
the plastic, infiltrated/embedded well, and sectioned great,
If anyone has an Immuno Job Description you are willing to share I would
appreciate it. My email address is carol.fie...@northside.com.
Thanks to all.
Carole
Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@northside.com
CONFIDENTIALITY NOTICE: T
Looking for the same
Ian
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carlos Hernandez
Sent: Tuesday, July 03, 2012 11:34 PM
To: Histonet
Subject: [Histonet] Immuno Controls
Hi All!
I was wondering if
Hi All!
I was wondering if there is anybody out there that has an over abundance of
controls for mart/melan a, s100, hmb45, pan keratin, ki-67, and mitf that they
are will to share or sell? Please let me know if you can help me out.
Thanks,
Carlos
_
We have the IntelliPath as well. I have the same sentiments as Linda regarding
all aspects of Biocare and the instrument. We use their antibodies and
detection. The company as a whole is excellent to deal with and very customer
oriented.
Thanks, Karen Lahti
Arizona Digestive Health
Phoenix
I have the Intellipath from BioCare. I love it. Service is spectacular. It's
very user friendly. You do have to be consistent with your cleaning protocol.
In the past 3-4 years I have only had 1 day of down time. The company is very
proactive when it comes to service. You couldn't ask for bette
Hello Histonetters
Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how
times flies when you have time off. Now it's back to the old laboratory
grind.
We are in the market for a new immuno stainer. I currently use a Dako Link,
which I am very pleased with. I have demoed the new
ith CD4, I
assume it is likely to do so with other finicky epitopes.
Have a nice day,
Amos
On Wed, Aug 3, 2011 at 1:00 PM,
wrote:
> Message: 7
> Date: Wed, 3 Aug 2011 11:49:40 -0500
> From: "Troutman, Kenneth A"
> Subject: [Histonet] Immuno. confusion
> To:
works so I don't argue with it.
R= egards,
Patsy
Original Message ----Subject: [Histonet] Immuno.
confusion
From: "Hannen, Valerie" >
Date: Wed, August 03, 2011 8:57 am
To: "[1]histonet@list
37232
From: Hannen, Valerie
Subject: [Histonet] Immuno. confusion
To: "histonet@lists.utsouthwestern.edu"
Date: Wednesday, August 3, 2011, 11:57 AM
Hi folks...
I am going to try to revamp out Immuno. procedure and am looking at a couple of
spec. sheets from our primary vendor. I am get
irst.
HIER will remove the formaldehyde cross linking and this step is required
before any other in the IHC protocol.
René J.
--- On Wed, 8/3/11, Hannen, Valerie wrote:
From: Hannen, Valerie
Subject: [Histonet] Immuno. confusion
To: "histonet@lists.utsouthwestern.edu"
Date: Wednesday
Hi folks...
I am going to try to revamp out Immuno. procedure and am looking at a couple of
spec. sheets from our primary vendor. I am gettin conflicting information...so
I am turning to you all for help.
When doing immuno's that require either HIER or enzyme digestion, do you
perform the retr
Also Sprach Zarathustra
René J.
From: Carrie Disbrow
To: histonet@lists.utsouthwestern.edu
Sent: Sunday, May 22, 2011 10:00 PM
Subject: [Histonet] Immuno controls
Hi all,
After consideration I disagree with the consensus and also agree with Curt's
pathologist. More supplies and
Hi all,
After consideration I disagree with the consensus and also agree with Curt's
pathologist. More supplies and labor will be involved as cutting more
controls will be neccessay to meet the requests of the pathologist. The patient
tissue is put on two controls slides. One slide is used
...@email.cs.nsw.gov.au
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of C B
Sent: Saturday, March 05, 2011 10:06 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] immuno stain for nerve in arterial wall
We had an investigator inquire about flourescent immuno stains for nerve in
arterial wall (not sure what species they are working with). I've never worked
with nerve before. Can someone give me some advice about what primary should I
be searching for? I realize I have to look in to species cr
IMMUNOHISTOCHEMISTRY TECHNICIAN
Sun - Thurs 12am - 8:30am
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas
is seeking an Immunohistochemistry Technician. Responsibilities include
slide preparation (paraffin and frozen sections),
manual IHC staining
Hi All,
Does anyone out there know a lab that is able to do a Human Herpes
Virus, Type 6 immuno?
Thanks for any help on this. We haven't been able to find a reference
lab that does this.
Carole
Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fie...@norths
ozeman
PO Box 173610
Bozeman MT 59717-3610
> To: histonet@lists.utsouthwestern.edu
> Date: Fri, 9 Apr 2010 09:11:59 -0400
> From: brusk...@aol.com
> Subject: [Histonet] Immuno staining on plastic sections
>
>
>
> We are trying to do immuno staining on plastic sections and
We are trying to do immuno staining on plastic sections and are not having much
luck. Does anyone have any experience with this? We are embedding in technovit
8100. It is a device with encapsulated VEGFR cells. I have been told that we
might have to do an etching step. Any help would be greatl
Does anyone use anti-trypsin (not alpha-1-anti-trypsin) for Acinar carcinoma on
paraffin IHC?
Thank you so much!
Paula Wilder
_
With Windows Live, you can organize, edit, and share your photos.
http://www.windowslive.com/Des
)
Immunohistochemistry Supervisor
University of Rochester
Department of Pathology
(585) 275-7210
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Diana McCaig
Sent: Fri 7/24/2009 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet
We had a series of PIN 4 cocktail immuno stains and got a poor reaction.
I am afraid to cut deeper in the block and miss the area of concern.
I have determined the problem was due to the heat bar not being turned
on.
What can I do the slides to reverse the process and restain them. The
DAB stain
...@lists.utsouthwestern.edu] On Behalf Of Jacobs,
Genie
Sent: Thursday, July 16, 2009 5:18 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] immuno staining
We use Premiere Charged Slides purchased from Cardinal and/or Mercedes for
our immuno stains. We recently purchased the Ven
We use Premiere Charged Slides purchased from Cardinal and/or Mercedes for our
immuno stains. We recently purchased the Ventana Ultra and really like it. We
dual mount the control and patient. We have recently had several cases where
the control was positive but the patient was not. We repea
Has anyone had any experience with an Immuno survey group out of Denmark
named NordiQC.
Judith Pardue
Memorial Health Care System
Chattanooga,Tn. 37404
This message and accompanying documents are covered by
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Subject: [Histonet] immuno set-up
To: histonet@lists.utsouthwestern.edu
Message-ID:
<
[EMAIL PROTECTED]
>
Content-Type: text/plain
Hello all,
I work at a private lab with one pathologist, and one histotech (me).
We do
about 5,000 cases a year. We are intrested in doin
: Thu, 20 Nov 2008 20:21:21 +
From: [EMAIL PROTECTED]
Subject: [Histonet] immuno set-up
To: histonet@lists.utsouthwestern.edu
Message-ID:
<
[EMAIL PROTECTED]
>
Content-Type: text/plain
Hello all,
I work at a private lab with one pathologist, and one histotech (me). We do
about
Hello all,
I work at a private lab with one pathologist, and one histotech (me). We do
about 5,000 cases a year. We are intrested in doing our immuno's in house
(right now we send them out), and wanted to know your opinion on the type of
equipment that would be best for this situation. Right no
I have a question for those doing immunos by hand. I typically dilute my
primary, secondary and tertiary antibodies with manufacturer supplied
diluent. Recently, I was running a test to determine the source of a
mysterious precipitate that had begun to appear on my slides (It has
since then disappe
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