Hi Renee,
Are you using two different fixatives for bone marrow vs lymph node? And, if
yes, have the assays been validated on both fixatives?
Teri Johnson, HT(ASCP)QIHC
Manager, Clinical Trial Testing
T +1 760 516 5954
teri.john...@navigatebp.com
Navigate BioPharma, Inc.
A Novartis
Hello Renee,
We do K & L by ISH on the Bond-III and BondMax with Refine detection
without issue on any of the tissues we have run (GI, skin, BM, LN). We have
a normal tonsil control section on every slide. Do you also run a positive
control on every slide? How does it perform? Next step would be
Hello histonet,
I am doing K ISH on BM and Lymphoma cases using the Leica Bond refine red
detection. The BM are staining beautifully however, the lymph nodes are not
staining at all in particular Follicular Lymphoma cases. Any suggestions?
Thank you
Hi,
Please I am wondering whether you guys run controls with your
Kappa/Lambda ISH.
Thanks,
Wilson
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Histonetters...
Would anyone performing ISH probes for Kappa Lambda on the BOND III be
willing to share their protocol for B+ fixed, decaled, paraffin-embedded bone
marrow core sections. I have tried a variety of different combinations and
times with ER1, ER2, Enzyme incubation, yielding
Hi: Is anyone running the new Kappa and Lambda ASRs on the Ventana
Benchmark and would be willing to share the protocols?
Thanks in advance,
--
*Marian L. Powers*
*Q*uality *L*aboratory *C*onsultants
*Doctors Pathology Services * **
c| 302.747.0580
o| 302.677. ext: 110
f | 302.677.0010
Would anyone be willing to share their protocols for the Kappa and
Lambda ISH? I cannot get rid of the eosinophilic background staining.
Thanks,
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office: 913-234-0576
How are you doing your ISH? A commercial manual kit, home-made kit,
instrument?
On Fri, Aug 5, 2011 at 8:31 AM, Johnson, Nacaela
nacaela.john...@usoncology.com wrote:
Would anyone be willing to share their protocols for the Kappa and
Lambda ISH? I cannot get rid of the eosinophilic
From: Mark Tarango [mailto:marktara...@gmail.com]
Sent: Friday, August 05, 2011 10:54 AM
To: Johnson, Nacaela
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Kappa/Lambda ISH background
How are you doing your ISH? A commercial manual kit, home-made kit,
instrument?
On Fri
@lists.utsouthwestern.edu
Subject: [Histonet] Kappa/Lambda ISH on Ventana
Hi Histonet!
I have been tasked with validating Kappa and Lambda on our Ventana
Benchmark
XT machines. Does anyone who uses this platform have suggestions as to
which Ventana protease works best (1, 2 or 3)? Additionally, any
: [Histonet] Kappa/Lambda ISH on Ventana
Ventana can no longer give out protocols. Theses probes are ASR products
and the FDA doesn't allow them to tell you the protocol. They can offer
guidance if you have a protocol that doesn't work well, but they can't
outright tell you a protocol. Another
Hi Histonet!
I have been tasked with validating Kappa and Lambda on our Ventana Benchmark
XT machines. Does anyone who uses this platform have suggestions as to
which Ventana protease works best (1, 2 or 3)? Additionally, any other
information concerning the optimization (suggested incubation
Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven
Wilkes
Sent: Thursday, March 31, 2011 3:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Kappa/Lambda
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