Re: [Histonet] Need help identifying a product!!!

2017-09-04 Thread Jay Lundgren via Histonet
, Inc.5830 N Blue Lake > DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www. > excaliburpathology.com > > From: "Cooper, Brian via Histonet" utsouthwestern.edu> > To: "histonet@lists.utsouthwestern.edu" utsouthwestern.edu> > Sent: Frida

Re: [Histonet] Need help identifying a product!!!

2017-09-01 Thread Paula Keene Pierce via Histonet
"histonet@lists.utsouthwestern.edu" Sent: Friday, September 1, 2017 1:34 PM Subject: [Histonet] Need help identifying a product!!! Happy Friday Histonet! Our cassette printed died this week, and we've been handwriting cassettes for the last couple of days.  It's been YEARS sinc

[Histonet] Need help identifying a product!!!

2017-09-01 Thread Cooper, Brian via Histonet
Happy Friday Histonet! Our cassette printed died this week, and we've been handwriting cassettes for the last couple of days. It's been YEARS since I had to do this; BOY has my handwriting atrophied since then! I remember we used to have this little metal rack that held the cassettes perfectl

Re: [Histonet] need help staining 120um human whole brain sections!

2017-03-20 Thread Maria Mejia via Histonet
Good morning Rene, I have worked out special staining IHC protocols to work on celloidin cut sections from 60um to 600um thick. These are free-floating human whole brainstem & whole brain sections. The IHC is amazing using single, double & triple. We save on antibodies, ABC, polymer, TSA using

Re: [Histonet] need help staining 120um human whole brain sections!

2017-03-20 Thread Rene J Buesa via Histonet
You have a special project → special tasks so your approach has to be equally special.Large brain sections are usually stained while floating but for IH with different and successive steps requiring very expensive reagents, floating sections is not well suited.You should affix the sections to la

[Histonet] need help staining 120um human whole brain sections!

2017-03-19 Thread Maria Mejia via Histonet
Or lab is currently processing a human whole brain. In about a month or two, the whole brain, which will be encased in celloidin & serial sections will be cut at 120um each. Now, we’ve bought an old Tetrander cast iron microtome. If you haven’t seen one of these microtomes, I can tell you it

[Histonet] Need help reviewing undecalcified bone slides

2015-05-19 Thread Hernandez, Jesus Willibaldo
Hi all, I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review.

[Histonet] need help on a cap question

2014-10-07 Thread Kolman, Kimberly D.
I could use some help clarifying this question: **NEW** 04/21/2014 ANP.11525 Tissue/Cytology Assessment Record Phase I If a statement of adequacy, preliminary diagnosis, or recommendations for additional studies is provided at the time of tissue and cytology sample collection, d

[Histonet] Need help with equipment for a New Laboratory

2014-09-22 Thread Mitchell Wan
Hi All, I am looking to purchase equipment to set up a new AP laboratory. Looking at second hand quality Leica : Tissue Processors Coverslippers Stainer - preferably ST4040 Any brand: Embedding Centres Low profile Manual microtomes Water Baths Cold Plates Anything to do with Histo

[Histonet] Need help with a publication reference

2014-01-29 Thread gayle callis
Dear Histonetters, If anyone has an original copy of Woods and Ellis, Laboratory Histopathology: A Complete Reference, 1994 Churchill Livingstone, I would like to know the chapter and page numbers for Anthony S-Y Leong, " Fixation and Fixatives" chapter. Title of book, publisher, date and IS

[Histonet] ## NEED HELP NEED JOB ##

2013-04-02 Thread Ali A Krasht
Hi Guys I am a LIS/ Histologist (HT & HTL ASCP Approved) and currently located in Frisco Texas. I graduated with a B.S. in Biology with a minor in Psychology and two yearsMedical Technology including Information Systems Technology and a part time Internet Marketing.  I have ten years of Histol

RE: [Histonet] Need help with spirochete control

2012-10-22 Thread Lynette Pavelich
: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Sheila Fonner [fourfonn...@yahoo.com] Sent: Monday, October 22, 2012 9:17 AM To: Histonet Subject: [Histonet] Need help with spirochete control Hello all, I am in need of some help with a spirochete

[Histonet] Need help with spirochete control

2012-10-22 Thread Sheila Fonner
Hello all, I am in need of some help with a spirochete protocol for the Leica Bond.  I am using a Biocare primary antibody.  I've tried ER1 for 20 min. with 15 min. Ab with no luck.  Does anyone do this, and do you think increasing the primary Ab time would help?  I've used this same Ab before o

[Histonet] Need help with CD40 on frozen sections

2012-07-09 Thread Bea DeBrosse-Serra
Histonetters, I need some help with CD40 on frozen sections (spleen, non-treated). I cut my frozens, let them briefly air dry and keep them at -20ºC. When I use them for staining, I air dry them, fix them in cold acetone for 5 minutes and start the staining process. I use the primary AB from BD

[Histonet] need help with TMA

2011-10-28 Thread Patricia F Lott
We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections. I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then min

[Histonet] Need help with Laminin

2011-10-17 Thread Perry, Margaret
I am trying to work up Laminin in dogs and cats. I have tried the antibody from Sigma L9393 with a variety of retrieval methods both enzymatic and HEIR with no success. I would like to hear from anyone who is testing for laminin. Do I need to use a different antibody? Margaret Perry HT(ASCP)

[Histonet] Need help with interpretation: Gomori Trichrome / MELAS-Syndrom

2011-02-10 Thread V. Neubert
Hi all, I'm reading about OXPHOS diseases (defects in mitochondrial protein subunits of respiratory chain). RFF in MERRF stands for "red ragged fibers", see: http://en.wikipedia.org/wiki/File:Ragged_red_fibers_in_MELAS.jpg I don't get what is stained there exactly. The picture shows a muscle biop

Re: [Histonet] Need help with p16 pricing

2011-01-12 Thread Richard Cartun
We use the mtm "predilute" mAb with excellent results. Due to the sensitivity of our detection (Leica-Microsystems' Bond Polymer Refine kit), we are able to dilute it to 1:10 and still obtain outstanding immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology D

[Histonet] Need help with p16 pricing

2011-01-11 Thread Rhonda Henshall-Powell
Hello All, I am having a hard time getting list price on MTM's p16 CINtec kit for histology - anyone using is out there? Is this the only IVD test on the market? What are people using? is it cost effective? CINtec® Histology: Part No. 9517 Thanks, Rhonda _

Fw: [Histonet] Need help with an old autostainer

2011-01-04 Thread Adrienne Anderson
Thanks for all the great responses. I have found a manual and lots of advice! Thanks, Adrienne - Forwarded Message From: Adrienne Anderson To: histonet@lists.utsouthwestern.edu Sent: Tue, January 4, 2011 12:19:13 PM Subject: [Histonet] Need help with an old autostainer Hello, I know

[Histonet] Need help with an old autostainer

2011-01-04 Thread Adrienne Anderson
Hello, I know this is probably a long shot, but I'm trying to set up a stainer and don't have a user manual. It was bought at auction by my company, so who knows how old it is! It is a Microm DS 50, made by Zeiss (now Thermo Richard Allen). I've been trying to get my hands on a manual (having t

Re: [Histonet] Need help

2010-06-02 Thread louise renton
Shirley go to this site for more information on supply, plug type etc http://www.kropla.com/electric2.htm On Tue, Jun 1, 2010 at 6:52 PM, Shirley A. Powell wrote: > Hi Histonetters, > > One of my pathologists is going on a mission to Haiti in July. She is > taking battery powered microscope to

[Histonet] Need help

2010-06-01 Thread Shirley A. Powell
Hi Histonetters, One of my pathologists is going on a mission to Haiti in July. She is taking battery powered microscope to use. She is not sure what type of power is available. All the manual says about the battery option is "the LED configuration is operational with an in-built re-chargeab

RE: [Histonet] Need help with FISH staining protocol on

2010-03-11 Thread Shen, Jinhui
Jinhui Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need help with FISH staining protocol on Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin, frosen nothinhg worked. I know other people that did not suceeded on other animals, I do not know anybody that could do it exept o

Re: [Histonet] Need help with FISH staining protocol on

2010-03-11 Thread J C
Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin, frosen nothinhg worked. I know other people that did not suceeded on other animals, I do not know anybody that could do it exept of somebody in German, that worked with special plasmid for rat. There are not repeated work

Re: [Histonet] Need help with FISH staining protocol on frozen tissue sections

2010-03-10 Thread Merced M Leiker
I have been using IDLabs porcine Y chromosome green label probe on frozen pig tissue with good results most of the time. You say you denature the probe and then hold it at 37oC...I would think that would allow the probe to reanneal to itself since you also hybridize later on at that temp...try

[Histonet] Need help with FISH staining protocol on frozen tissue sections

2010-03-09 Thread Shen, Jinhui
Hi everyone, Has anyone done FISH staining on mice tissue frozen sections for Y chromosome? I need help with a successful protocol. Recently I started to do FISH staining on mice liver frozen sections for chromosome Y, but got no positive results even on male samples yet. The probe I used was

Re: [Histonet] need help with stain

2010-01-28 Thread Rene J Buesa
Use mucicarmin stain. The crypt mucus will be evident and  their sizes. René J. --- On Thu, 1/28/10, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] need help with stain To: "histonet@lists.utsouthwestern.edu" Date: Thursday, January 28, 2010, 4:00 PM A researche

[Histonet] need help with stain

2010-01-28 Thread Perry, Margaret
A researcher wants to measure the length of the intestinal crypts and asked for a suggestion on what type of stain to use. I am thinking of using a phloxine/tartrazine stain and do a double stain with a GMS to show the basement membrane. What is your opinion on this? Do you have other suggest

Re: [Histonet] need help in selecting blades for MicrotomeLeitz1512and good place for service

2009-10-20 Thread Andrea Grantham
ubject: RE: [Histonet] need help in selecting blades for MicrotomeLeitz1512and good place for service Hi Bader, I've been a Histotechnologist for 30 years, and the Leitz 1512 is the best microtome I have ever used. (And I've used a lot)I can easily cut 1-2 micron sections of any tissue, inc

RE: [Histonet] need help in selecting blades for Microtome Leitz1512and good place for service

2009-10-20 Thread Bonner, Janet
...@lists.utsouthwestern.edu on behalf of Bader Siddiki Sent: Mon 10/19/2009 6:36 PM To: Histonet Subject: [Histonet] need help in selecting blades for Microtome Leitz1512and good place for service Hello Histonets I need some help and advice from you experts. We are new in cutting formalin fixed

RE: [Histonet] need help in selecting blades for MicrotomeLeitz1512and good place for service

2009-10-20 Thread Weems, Joyce
...@lists.utsouthwestern.edu] On Behalf Of Armandi, Arlene Sent: Tuesday, October 20, 2009 10:15 To: Bader Siddiki; Histonet Subject: RE: [Histonet] need help in selecting blades for MicrotomeLeitz1512and good place for service Hi Bader, I've been a Histotechnologist for 30 years, and the Leitz 1512 is the best microt

RE: [Histonet] need help in selecting blades for Microtome Leitz1512and good place for service

2009-10-20 Thread Armandi, Arlene
hs.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bader Siddiki Sent: Monday, October 19, 2009 3:37 PM To: Histonet Subject: [Histonet] need help in selecting blades for Microtome Leitz1512and good place f

[Histonet] need help in selecting blades for Microtome Leitz1512 and good place for service

2009-10-19 Thread Bader Siddiki
Hello Histonets I need some help and advice from you experts. We are new in cutting formalin fixed paraffin embedded (FFPE) tissue sections. We need your advice. We have a used Leitz 1512 microtome. --- perhaps it needs service, can you recommend a good and reasonable place. what will

[Histonet] Need help on Streptavidin-HRP conjugate for IHC

2009-08-14 Thread Karen Cai
Hi, I am using ABC kit for DAB-IHC experiment. But somehow, I don't feel it is a good choice since the background is kinda high. Is there anybody having some suggestions? Which kind of steptavidin-HRP are you using for DAB-IHC? From which company? Thank you very much for your kind reply, Best

[Histonet] Need Help

2009-07-02 Thread Pamela Marcum
I need to reach Charlie Dorner.  If anyone can get her this message I would appreciate it.  Thanks, Pam Marcum ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] need help with Elastase staining

2009-02-09 Thread Patricia F Lott
Does anyone have any experience with staining for Elastase in Neutrophils in tissue sections? Any help would be appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Need help on understanding the pro and cons of a Leica CM3600 XP crymacrotome.

2009-01-05 Thread Gamal Akabani
Hello there: I am researching the Leica CM3600 XP cryomacrotome for a small-animal imaging research facility and I would like to know who has experience with this machine. I thank you in advanced for all the help I can get. Gamal Akabani ___ Histonet

RE: [Histonet] Need help for CD95(FAS) and antigen retrieval system

2008-10-26 Thread Patsy Ruegg
siddiqui Sent: Friday, October 24, 2008 12:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help for CD95(FAS) and antigen retrieval system Hi everyone, I am sure some one in this large world of histonet will be able to help me. I am trying to do CD95 staining on human skin FF

[Histonet] Need help for CD95(FAS) and antigen retrieval system

2008-10-24 Thread soofia siddiqui
Hi everyone, I am sure some one in this large world of histonet will be able to help me. I am trying to do CD95 staining on human skin FF PE tissue sections. CD95 is 45 KD single chain type cell surface  protein that mediates apoptosis when crosslinked with agnostic anti-Fas antibodies.I have tr

Re:[Histonet] need help for cultured cells

2008-10-23 Thread AGrobe2555
You'll need to resuspend your pellet in a limited volume, do a cell count (using a hemocytometer, etc) and dilute the original suspension to the desired cell#/ul concentration with an appropriate diluent. Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Mo

[Histonet] need help for cultured cells

2008-10-23 Thread Nejat Yilmaz
Dear Netters, How can I set 50.000 cells in 1 ul suspension from a cultured cell pellet? I need your kindly help. Best regards... Dr. Necat Yilmaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman