, Inc.5830 N Blue Lake
> DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.
> excaliburpathology.com
>
> From: "Cooper, Brian via Histonet" utsouthwestern.edu>
> To: "histonet@lists.utsouthwestern.edu" utsouthwestern.edu>
> Sent: Frida
"histonet@lists.utsouthwestern.edu"
Sent: Friday, September 1, 2017 1:34 PM
Subject: [Histonet] Need help identifying a product!!!
Happy Friday Histonet!
Our cassette printed died this week, and we've been handwriting cassettes for
the last couple of days. It's been YEARS sinc
Happy Friday Histonet!
Our cassette printed died this week, and we've been handwriting cassettes for
the last couple of days. It's been YEARS since I had to do this; BOY has my
handwriting atrophied since then! I remember we used to have this little metal
rack that held the cassettes perfectl
Good morning Rene,
I have worked out special staining IHC protocols to work on celloidin cut
sections from 60um to 600um thick. These are
free-floating human whole brainstem & whole brain sections. The IHC is amazing
using single, double & triple. We save on
antibodies, ABC, polymer, TSA using
You have a special project → special tasks so your approach has to be equally
special.Large brain sections are usually stained while floating but for IH with
different and successive steps requiring very expensive reagents, floating
sections is not well suited.You should affix the sections to la
Or lab is currently processing a human whole brain. In about a month or two,
the whole brain, which will be encased
in celloidin & serial sections will be cut at 120um each. Now, we’ve bought an
old Tetrander cast iron microtome. If
you haven’t seen one of these microtomes, I can tell you it
Hi all,
I just wanted to know if anyone out there had experience reviewing
undecalcified bone sections. I still feel like the morphology is not showing up
on my sections so I am in need of assistance in troubleshooting this issue. I
just uploaded two pictures on the histonet server for review.
I could use some help clarifying this question:
**NEW** 04/21/2014
ANP.11525
Tissue/Cytology Assessment Record
Phase I
If a statement of adequacy, preliminary diagnosis, or recommendations
for additional studies is provided at the time of tissue and cytology
sample collection, d
Hi All,
I am looking to purchase equipment to set up a new AP laboratory.
Looking at second hand quality Leica :
Tissue Processors
Coverslippers
Stainer - preferably ST4040
Any brand:
Embedding Centres
Low profile Manual microtomes
Water Baths
Cold Plates
Anything to do with Histo
Dear Histonetters,
If anyone has an original copy of Woods and Ellis, Laboratory
Histopathology: A Complete Reference, 1994 Churchill Livingstone, I would
like to know the chapter and page numbers for Anthony S-Y Leong, " Fixation
and Fixatives" chapter. Title of book, publisher, date and IS
Hi Guys
I am a LIS/ Histologist (HT & HTL ASCP Approved) and currently located in
Frisco Texas. I graduated with a B.S. in Biology with a minor in Psychology and
two yearsMedical Technology including Information Systems Technology and a part
time Internet Marketing.
I have ten years of Histol
: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Sheila Fonner
[fourfonn...@yahoo.com]
Sent: Monday, October 22, 2012 9:17 AM
To: Histonet
Subject: [Histonet] Need help with spirochete control
Hello all,
I am in need of some help with a spirochete
Hello all,
I am in need of some help with a spirochete protocol for the Leica Bond. I am
using a Biocare primary antibody. I've tried ER1 for 20 min. with 15 min. Ab
with no luck. Does anyone do this, and do you think increasing the primary Ab
time would help? I've used this same Ab before o
Histonetters,
I need some help with CD40 on frozen sections (spleen, non-treated). I cut my
frozens, let them briefly air dry and keep them at -20ºC. When I use them for
staining, I air dry them, fix them in cold acetone for 5 minutes and start the
staining process. I use the primary AB from BD
We have recently started doing tissue microarrays. The problem we have is that
some of the small cores drop out after the first few sections.
I think my problem is in the step where we are supposed to melt the donor and
recipient blocks together. We tried 5 minutes at 37 degrees, and then min
I am trying to work up Laminin in dogs and cats. I have tried the antibody
from Sigma L9393 with a variety of retrieval methods both enzymatic and HEIR
with no success. I would like to hear from anyone who is testing for laminin.
Do I need to use a different antibody?
Margaret Perry HT(ASCP)
Hi all,
I'm reading about OXPHOS diseases (defects in mitochondrial protein
subunits of respiratory chain). RFF in MERRF stands for "red ragged
fibers", see:
http://en.wikipedia.org/wiki/File:Ragged_red_fibers_in_MELAS.jpg
I don't get what is stained there exactly. The picture shows a muscle
biop
We use the mtm "predilute" mAb with excellent results. Due to the
sensitivity of our detection (Leica-Microsystems' Bond Polymer Refine
kit), we are able to dilute it to 1:10 and still obtain outstanding
immunoreactivity.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
D
Hello All,
I am having a hard time getting list price on MTM's p16 CINtec kit for
histology - anyone using is out there? Is this the only IVD test on the
market? What are people using? is it cost effective?
CINtec® Histology: Part No. 9517
Thanks,
Rhonda
_
Thanks for all the great responses. I have found a manual and lots of advice!
Thanks,
Adrienne
- Forwarded Message
From: Adrienne Anderson
To: histonet@lists.utsouthwestern.edu
Sent: Tue, January 4, 2011 12:19:13 PM
Subject: [Histonet] Need help with an old autostainer
Hello,
I know
Hello,
I know this is probably a long shot, but I'm trying to set up a stainer and
don't have a user manual. It was bought at auction by my company, so who knows
how old it is! It is a Microm DS 50, made by Zeiss (now Thermo Richard Allen).
I've been trying to get my hands on a manual (having t
Shirley
go to this site for more information on supply, plug type etc
http://www.kropla.com/electric2.htm
On Tue, Jun 1, 2010 at 6:52 PM, Shirley A. Powell wrote:
> Hi Histonetters,
>
> One of my pathologists is going on a mission to Haiti in July. She is
> taking battery powered microscope to
Hi Histonetters,
One of my pathologists is going on a mission to Haiti in July. She is taking
battery powered microscope to use. She is not sure what type of power is
available. All the manual says about the battery option is "the LED
configuration is operational with an in-built re-chargeab
Jinhui
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Need help with FISH staining protocol on
Dear Jinhui! We tried for a long time to perform SRY FISH for rats, paraffin,
frosen nothinhg worked. I know other people that did not suceeded on other
animals, I do not know anybody that could do it exept o
Dear Jinhui! We tried for a long time to perform SRY FISH for rats,
paraffin, frosen nothinhg worked. I know other people that did not suceeded
on other animals, I do not know anybody that could do it exept of somebody
in German, that worked with special plasmid for rat. There are not repeated
work
I have been using IDLabs porcine Y chromosome green label probe on frozen
pig tissue with good results most of the time. You say you denature the
probe and then hold it at 37oC...I would think that would allow the probe
to reanneal to itself since you also hybridize later on at that temp...try
Hi everyone,
Has anyone done FISH staining on mice tissue frozen sections for Y chromosome?
I need help with a successful protocol.
Recently I started to do FISH staining on mice liver frozen sections for
chromosome Y, but got no positive results even on male samples yet. The probe I
used was
Use mucicarmin stain. The crypt mucus will be evident and their sizes.
René J.
--- On Thu, 1/28/10, Perry, Margaret wrote:
From: Perry, Margaret
Subject: [Histonet] need help with stain
To: "histonet@lists.utsouthwestern.edu"
Date: Thursday, January 28, 2010, 4:00 PM
A researche
A researcher wants to measure the length of the intestinal crypts and asked for
a suggestion on what type of stain to use. I am thinking of using a
phloxine/tartrazine stain and do a double stain with a GMS to show the basement
membrane. What is your opinion on this?
Do you have other suggest
ubject: RE: [Histonet] need help in selecting blades for
MicrotomeLeitz1512and good place for service
Hi Bader,
I've been a Histotechnologist for 30 years, and the Leitz 1512 is the
best microtome I have ever used. (And I've used a lot)I can easily cut
1-2 micron sections of any tissue, inc
...@lists.utsouthwestern.edu on behalf of Bader Siddiki
Sent: Mon 10/19/2009 6:36 PM
To: Histonet
Subject: [Histonet] need help in selecting blades for Microtome Leitz1512and
good place for service
Hello Histonets
I need some help and advice from you experts.
We are new in cutting formalin fixed
...@lists.utsouthwestern.edu] On Behalf Of Armandi,
Arlene
Sent: Tuesday, October 20, 2009 10:15
To: Bader Siddiki; Histonet
Subject: RE: [Histonet] need help in selecting blades for
MicrotomeLeitz1512and good place for service
Hi Bader,
I've been a Histotechnologist for 30 years, and the Leitz 1512 is the
best microt
hs.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bader
Siddiki
Sent: Monday, October 19, 2009 3:37 PM
To: Histonet
Subject: [Histonet] need help in selecting blades for Microtome
Leitz1512and good place f
Hello Histonets
I need some help and advice from you experts.
We are new in cutting formalin fixed paraffin embedded (FFPE) tissue
sections.
We need your advice.
We have a used Leitz 1512 microtome.
--- perhaps it needs service, can you recommend a good and reasonable
place.
what will
Hi,
I am using ABC kit for DAB-IHC experiment. But somehow, I don't feel it
is a good choice since the background is kinda high. Is there anybody
having some suggestions? Which kind of steptavidin-HRP are you using for
DAB-IHC? From which company?
Thank you very much for your kind reply,
Best
I need to reach Charlie Dorner. If anyone can get her this message I would
appreciate it.
Thanks,
Pam Marcum
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Does anyone have any experience with staining for Elastase in
Neutrophils in tissue sections? Any help would be appreciated!
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hello there:
I am researching the Leica CM3600 XP cryomacrotome for a small-animal
imaging research facility and I would like to know who has experience
with this machine. I thank you in advanced for all the help I can get.
Gamal Akabani
___
Histonet
siddiqui
Sent: Friday, October 24, 2008 12:10 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Need help for CD95(FAS) and antigen retrieval system
Hi everyone,
I am sure some one in this large world of histonet will be able to help me.
I am trying to do CD95 staining on human skin FF
Hi everyone,
I am sure some one in this large world of histonet will be able to help me. I
am trying to do CD95 staining on human skin FF PE tissue sections. CD95 is 45
KD single chain type cell surface protein that mediates apoptosis when
crosslinked with agnostic anti-Fas antibodies.I have tr
You'll need to resuspend your pellet in a limited volume, do a cell count
(using a hemocytometer, etc) and dilute the original suspension to the desired
cell#/ul concentration with an appropriate diluent.
Albert
Albert C. Grobe, PhD
Tissue Engineering Lab
International Heart Institute of Mo
Dear Netters,
How can I set 50.000 cells in 1 ul suspension from a cultured cell pellet? I
need your kindly help.
Best regards...
Dr. Necat Yilmaz
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman
42 matches
Mail list logo
