Hi,
I am having some trouble with Nissl staining newborn (P0) mouse heads/hindbrain.
The nissl stain appears very faint in the centre of my sections. (Paraffin
embedded, 7um thick)
Is this likely to be a penetration issue?
I fixed in 4% PFA O/N before processing - Would that affect it?
I left
Did you decal the skull?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 Eas= t Riverside Dr. Bldg 4 Suite 100
= Austin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] Nissl Stain P0 Mouse Head
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