Hi Carol,
We can do that here; I am copying an internal e-mail I got from the pathologist
that runs our EM. Hope this helps:
Regards, Mike
***
Mike,
Can you follow up on this and give me some idea of projected number of cases? I
would be interested in talking with Carol Wilson if you h
Hi All,
Can anyone recommend a reference lab that can use paraffin sections or formalin
fixed tissue for EM?
Thanks in advance for suggestions.
Carol
Carol Wilson, HT(ASCP)
Associate Scientist III
Team Leader/Histopathology
Ricerca Biosciences, LLC
___
17, 2013 10:43:02 AM
Subject: [Histonet] Paraffin sections mouse brain
Hi everyone:
While I have sectioned a lot of different stuff, I have not done mouse brain
before and I am having considerable difficulty. The brains are fixed and
were embedded by our pathology department on their aut
Hi everyone:
While I have sectioned a lot of different stuff, I have not done mouse brain
before and I am having considerable difficulty. The brains are fixed and
were embedded by our pathology department on their automated system. When
cutting, the paraffin cuts nicely but the brain tissue shre
Hi!
For our KRAS-testing we clean the microtome with LTK008, especially surfaces
that come in contact with the sections. We use one-way-toothpicks, single
packed, to pick the slide from the blade and give it directly into a sterile
collection tube. 1-10 sections are collected depending on the dimen
lar water is cheaper
and don't stink like DEPC!
Good luck,
Amos
On Tue, Oct 4, 2011 at 1:00 PM,
wrote:
> Message: 2
> Date: Tue, 4 Oct 2011 16:44:17 +
> From: "Crowell, Thomas"
> Subject: [Histonet] Paraffin sections for molecular assays
> To: &quo
Dear Histonetters,
Can you tell me what procedures you use to prevent DNA/RNA contamination of
tissue sections when handling multiple samples. Do you just wipe down blade
holder and blade (or use a new blade) between samples, or do you use more
stringent cleaning?
Thomas Crowell
Diagnostic D
Dear collegues,
I am experiencing following problem.
I have embedded hypothalamus tissue in paraffin using the following procedure:
-fixation in 4% paraformaldehyde for 48 hours, fixation of the tissue in 50%
alcohol, next day in 70% alcohol,next day paraffin embedding.
During the paraffin embed
: Nagappan, Peri
Cc: Histonet
Subject: Re: [Histonet] Paraffin Sections
Sent: Aug 4, 2009 1:40 PM
Peri,
Sounds like poor infiltration to me. What kind of tissue are we talking
about, and what processing program did you use?
Kathleen
Principal Lab Technician
Neurotoxicology Labs
Dept of
Peri,
Sounds like poor infiltration to me. What kind of tissue are we talking
about, and what processing program did you use?
Kathleen
Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Rd
Piscataway, NJ 08854
Nagap
Hi Histonetters,
When I cut the paraffin sections in the microtome, I am not getting the whole
sections intact, rather some portion in the middle of the sections are brittle.
But the paraffin portion surrounds the tissue is smooth, nice and intact.
Thanks for your suggestion and help.
Hi James,
While I know that others with more experience are going to reply and have
very good insights to add, in my few years of experience I've stored the
paraffin sections at room temp. for up to several months. Sections are
stored only after baking them in a 60 C for 1 hour. This baking
I am a beginning to do paraffin section. I need to know the following as I have
heard many things and I have received my best advice here. As of this moment I
was storing them at 4 degrees following sections. The problem I am having is
many of my section have been coming off when I deparaffinize
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