Hi All,
Hope all are having a great Monday
I am trying to do a Picro Sirius Red stain and have looked at many protocols
that seem to not use a particular reagent that is in a manufacturers kit. I was
hoping if someone could enlighten me as to why this reagent would be used and
if someone
I do a Picro Sirius Red stain for collagen (got the protocol from Gayle Callis)
and don't use Phosphomolybdic acid.
My protocol is simple:
one hour in PSR
two rinses in acidified water
rinse
you can counterstain but my clients don't want a counterstain so I dehydrate,
clear and coverslip.
Boom,
Dear John,
I usually perform Picrosirius Red stain in cardiac tisse sections.
I use phosphomolibic acid treatment in order to eliminate the cytoplasmic
staining improving collagen/myoplasm contrast.
Have a look at this paper:
Picrosirius red staining of cardiac muscle following phosphomolybdic
