wrote:
>
> Good Morning,
>
> I would like to post a question on histonet:
>
> My organization is made up of multiple hospitals and surgery centers. All
> specimens are couriered to the Pathology Department which is located at only
> one of the facilities. The sit
Good Morning,
I would like to post a question on histonet:
My organization is made up of multiple hospitals and surgery centers. All
specimens are couriered to the Pathology Department which is located at only
one of the facilities. The sites without a fully operational pathology lab
provide
The longer the tissue sits in paraformaldehyde the more the proteins in the
tissues will become cross-linked due to multiple methylene bridge
formations and could require very rigorous antigen retrieval or may even be
beyond retrieval; the tissues could become brittle as well due to
Merced is correct about longer fixation inducing more cross-linking,
possibly making immunostaining more difficult.
Much depends on the antigen you are looking for.
I disagree that the tissue will become brittle, this is not my experience.
Geoff
Salim Yalcin Inan wrote:
Hello,
I have a
Yalcin Inan
Sent: Wednesday, September 22, 2010 5:39 PM
To: Histonet
Subject: [Histonet] post-fixation question
Hello,
I have a question about post-fixation. As far as I know, for most of the
immunohistochemistry studies in mouse/rat brain, post-fixation period is
between 2 hours to 24 hours
Hello,
I have a question about post-fixation. As far as I know, for most of the
immunohistochemistry studies in mouse/rat brain, post-fixation period is
between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius followed by
cryoprotection with 20-30% sucrose solution. Are there any
