Hi Martha
I think that Caroline is correct but you have nothing to lose by removing
the wax and then trying to dehydrate and so on. A more gentle way after
removing the wax and dehydrating is to use chloroform instead of xylene and
hand processing. Chloroform is much more gentle although tissue do
Having morphology problems when "re-processing" a tissue after an accident as
you had is the "unavoidable" consequence. The problem is rooted in the fact
that usually you remove the paraffin using the tissue processor cleaning cycle
and that is a very harsh method (albeit the most expeditious).
I am afraid your tissue might be 'toast'. I tried to reprocess from that
same issue and things did not go well. We were processing mouse tissue,
which is dry enough, but then when we put the tissues back through xylene
and into 100% and back again it got even worse.
We had to just get what we coul
I am Martha Davenport, supervisor, at the University of KY histology lab. We
had a processing problem caused by accidently placing 70% (instead of 100%)
into the last dehydration container. Would anyone please give me info on how
you would remedy this? We usually reprocess the tissue but have
M
Subject: [Histonet] Processing Problem
Hi All,
I need help with two issues. I have a researcher who is sending
us porcine skin, about 2mm x 2mm x 1mm tall.
Issue 1: There is a lesion in the center, and although he wants the skin
sectioned through the lesion eventually, he says if
Hi All,
I need help with two issues. I have a researcher who is sending
us porcine skin, about 2mm x 2mm x 1mm tall.
Issue 1: There is a lesion in the center, and although he wants the skin
sectioned through the lesion eventually, he says if he bisects the chunk of
skin befor
Hi everyone,
We are having a problem with a processing artifact that looks like very pale
staining in splotches just above the junction between the epidermis and dermis
but not extending all the way to the skin surface, and not everywhere at the
position. One of the processors had a clog in a
Hi All,
My Pathologist showed me several slides from the past few weeks that have a
strange appearance in the fatty areas. The fat cells are squished together and
don't have the open areas they usually have. Any ideas as to what is
happening? And we haven't changed our processing schedule. T
