I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here is the web page where you can find the formula.
http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/
I use it with very good results.
Read this page about eosin counterstaining on StainsFile.
Bryan Llewellyn
Cesar Francisco Romero wrote:
I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here is the web page where you can find the formula.
Silly me: http://stainsfile.info/StainsFile/stain/hematoxylin/h-and-e-eo.htm
Bryan
Bryan Llewellyn wrote:
Read this page about eosin counterstaining on StainsFile.
Bryan Llewellyn
Cesar Francisco Romero wrote:
I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here
I too have been experiencing Eosin leaching. I have tried all different
options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols
and xylenes that follow, even added a running water wash step after the Eosin (
to remove excess). Before this, I have had no problems and had
Subject: [Histonet] RE: Eosin Leaching
I too have been experiencing Eosin leaching. I have tried all different
options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols
and xylenes that follow, even added a running water wash step after the Eosin (
to remove excess). Before
Hi Adrienne,
We see eosin leaching in our summer months when it is very humid in our lab.
The humidity is 70% for most of the summer. We change out all of our alcohols
daily to try and prevent it but we sometimes see it even with doing that.
Rhonda Gregoire, MLT
Supervisor, Clinical
This exact reason is how I convinced my lab to go back to xylene!! I figure
it's because there is water in your substitute. Because you can't see if there
is water in it (from the air moisture or wherever...) it becomes frusterating
and annoying to have to change out to fresh solutions
dry.
Tresa
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Wednesday, March 27, 2013 8:11 AM
To: Gerard Spoelstra; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Eosin
Ceri,
There are several ways to darken the eosin counterstain:
1. Stain longer in eosin, though with some eosins including eosin Y, this
might not necessarily darken the staining since the eosin Y is a yellow red,
eosin B seems to be more bluish red
2. Under-differentiate the
Supposedly eosin used to color small specimens, or put in the
processor, fluoresces enough in tissue sections to interfere with
fluorescent stains.
I've seen safranin O (from the microbiology lab) used to mark small
surgical specimens like GI biopsies. I don't know whether you can put
it in the
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Sunday, September 09, 2012 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol
I just wanted to thank everyone who has responded to my message. All of the
input is greatly appreciated. This is a great site to be utilized in our quest
for Histology perfection.
Valerie
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington
Buy one with Phloxine in it. It will make it more red and stronger...
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From:
-boun...@lists.utsouthwestern.edu] on behalf of Sarah Dysart
[sdys...@mirnarx.com]
Sent: Thursday, August 09, 2012 11:27 AM
To: Hannen, Valerie; histonet
Subject: [Histonet] RE: Eosin
Buy one with Phloxine in it. It will make it more red and stronger...
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Thursday, August 09, 2012 10:28 AM
To: Hannen, Valerie; histonet
Subject: [Histonet] RE: Eosin
Buy one with Phloxine in it. It will make it more red
How about the thickness of the tissue? Once we were cutting skins at 3 microns
and the pathologist complained the Eosin was too light so we started cutting
them at 4-5 microns.
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC
Wanda makes an excellent point, thinner sections have less contrast.
Geoff
On 8/9/2012 2:50 PM, wanda.sm...@hcahealthcare.com wrote:
How about the thickness of the tissue? Once we were cutting skins at 3 microns
and the pathologist complained the Eosin was too light so we started cutting
We have used a mixture of Eosin Y and Phloxine B with acetic acid as follows:
780mL 95 %ETOH
100mL Eosin
10mL Phloxine B
4mL glacial acetic acid
We seem to be consistent. We have used this for 17 years.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
We use eosin in our last 100% alcohol on the processor. It is a great help for
our person embedding to be able to see and orient the small biopsies. We have
been doing this for years and have never had any problem with the processor or
any subsequent staining that has been performed. We also
We also add liquid eosin (~75mL) to our dirtiest 100% to help aide the sight
of the small biopsies for embedding. You are correct though, that eosin does
fluoresce.
I understand why you would be concerned about FISH, so wanted to share this. I
have heard from fellow techs in the field that
Here is a paper I found:
Capillary Electrophoresis Artifact Due to Eosin
Journal of Molecular Diagnostics, Vo. 7, No. 1, February 2005
Our molecular people are studying this issue to see how big of a problem it is.
Tim Morken
Supervisor, Electron Microscopy
Department of Pathology
UC San
Carol Freeman asks about using eosin and other dyes to mark small
specimens for better recovery during embedding.
I've found safranin O to be the best of these. Use the Gram stain
counterstain used in microbiology. You mark the specimens directly, on
those blue biopsy pads. It doesn't dissolve
Allison Scott at LBJ Hospital in Houston, Texas asks about the use of
eosin to dye small biopsy specimens.
Several replies mention addition of eosin to one of the processing
alcohols. I have never seen this done, in maybe 60 pathology services
I've worked in. (I'd know, because I nearly always
@lists.utsouthwestern.edu
cc
Subject
[Histonet] Re: Eosin to dye small Biopsies
Allison Scott at LBJ Hospital in Houston, Texas asks about the use of
eosin to dye small biopsy specimens.
Several replies mention addition of eosin to one of the processing
alcohols. I have never seen this done, in maybe
...@lists.utsouthwestern.edu
10/22/2010 10:44 AM
To
histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] Re: Eosin to dye small Biopsies
Allison Scott at LBJ Hospital in Houston, Texas asks about the use of
eosin to dye small biopsy specimens.
Several replies mention addition of eosin to one
We put it in our 95%
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildrens.org
-Original
Hi Scott,
Your discription of eosin leaching out after a few days is classic for the
possibility of bleach contamination on your staining containers. There are
multiple possibilities of other causes, but this could be a simple cause,
easily solved.
Renee Grow, BA., HT (ASCP)
rg...@bmnet.com
Cheryl,
Yes, the Lecia XL is supposed to vibrate when it lifts the racks. If it's not
doing that, you need to call and ask for some assistance. It sounds to me like
there is something either turned off or broken in the setup.
Regarding the eosin getting the alcohol pink, that happens to us
Thanks for all your help. It makes sense that it would evaporate faster on the
Leica as opposed to hand staining. The eosin on the Leica is uncovered for 6
plus hours a day when hand staining it was covered except when we were staining
a rack. I will add 100 alcohol as needed. Again thanks for
Hi Cheryl,
Maybe the auto stainer does not drain the rack a well or it could just be a
matter of the dipping and dunking that is causing more evaporation.
Jan
Omaha, NÉE
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
Eosin is alcohol-based, thus the evaporation. The containers on my
autostainer get covered as soon as the last run is finished and that
slows evaporation somewhat but I do replenish as needed. I like my eosin
level in the container a little lower so we don't have pink-stained
frosted ends, but
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, January 15, 2010 10:22 AM
To: Mahoney,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.
Eosin is alcohol-based, thus
,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.
I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H E since the frosted end of the slides were getting stained as well.
We also try
, January 15, 2010 11:14:30 AM
Subject: RE: [Histonet] RE: Eosin evaporation.
We do this as well, that is why I am stumped.
Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148
-Original Message-
From: Sherwood, Margaret [mailto:msherw
I have had some great suggestions from many of you, the problem is I am doing
literally all of them. My question is on the Leica XL isn't the instrument
supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why
it is keeping a lot of carryover because it isn't vibrating.
I think that you should talk with the person who sold you this refurbished
instrument and present your problem. That will be more expeditious.
René J.
--- On Fri, 1/15/10, Cheri Miller cmil...@physlab.com wrote:
From: Cheri Miller cmil...@physlab.com
Subject: [Histonet] re; eosin
To: histonet
: [Histonet] re; eosin
I have had some great suggestions from many of you, the problem is I am doing
literally all of them. My question is on the Leica XL isn't the instrument
supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it
is keeping a lot of carryover because it isn't
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great tool
to use for embedding. However, we received a letter from the lab that we send
our prostate biopsies to saying that it was undesirable
@lists.utsouthwestern.edu
cc
Subject
[Histonet] Re: Eosin in alcohol
We have used Eosin in the last 95% alcohol on the tissue processor for
several years. I usually add approximately 5 ml to the full jug. It is a
great tool to use for embedding. However, we received a letter from the
lab that we send our
process of staining
immunos.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson
Sent: Wednesday, July 01, 2009 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Eosin
, 2009 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Eosin in alcohol
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great tool
to use for embedding. However, we received
I think they are probably getting weak unwanted staining with P504s/AMACR
antibody on benign glands on their PIN4s.
Since this antibody is usually pink/red, it sounds like someone might have
decided that the unwanted color came from eosin in the processor, but this
would be the wrong conclusion.
jmjohnso...@hotmail.com
Subject: [Histonet] Re: Eosin in alcohol
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, July 1, 2009, 1:06 PM
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great
...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
[lbla...@digestivespecialists.com]
Sent: Wednesday, July 01, 2009 1:47 PM
To: 'Jennifer Johnson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Eosin in alcohol
We use about the same amount or eosin in the first 100% alcohol and also do
immunos
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