-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Thursday, January 22, 2015 2:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Re: Embedding
That is so cool!! I wish I had one of those!!
"By bitching and bi
der by reply email and destroy all
> copies of the original message.
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
> Sent: Wednesday, January 21, 2015 8:29 AM
> To: gayle.cal...@bresn
ssage-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Thursday, January 22, 2015 11:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Embedding
We use empty pipette tip boxes. They are t
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Thursday, January 22, 2015 10:14 AM
To: gayle.cal...@bresnan.net; 'Tony Auge'; histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa'
Subject
...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Thursday, January 22, 2015 1:14 PM
To: gayle.cal...@bresnan.net; 'Tony Auge'; histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa'
Subject: RE: [Histonet] Re: Embedding
We use a
estern.edu; 'Morken, Timothy'; 'Goins, Tresa'
Subject: RE: [Histonet] Re: Embedding
A fabulous idea! I suspect one could use a cheap travel iron although one
needs to devise a way to collect melted paraffin. Even our fancy para trimmer
didn't have catch pan for paraffin
-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge
Sent: Thursday, January 22, 2015 9:58 AM
To: histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa
Subject: Re: [Histonet] Re: Embedding
If you want a cheaper alternative you can use a ski wax iron.
If you want a cheaper alternative you can use a ski wax iron. They cost
about $40. I mounted one upside down in a bucket and it works just as well
as the $500 para trimmers.
-Tony Auge HTL (ASCP) QIHC
___
Histonet mailing list
Histonet@lists.utsouthwes
esday, January 21, 2015 8:29 AM
To: gayle.cal...@bresnan.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Embedding
I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest
price by far - and are saving our finger joints. The amount of wax remaining
on the cas
Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders,
Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, January 21, 2015 8:39 AM
To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: embe
e.cal...@bresnan.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Embedding
I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest
price by far - and are saving our finger joints. The amount of wax remaining
on the cassette also appears to depend on the brand of
Honestly, the easiest thing I think is just don't overfill the base mold. A
small amount in the bottom, then the tissue, then the cassette top and just top
off enough paraffin to cover. Unless you accidentally slosh it as you are
moving it to the cooling tray you will always have clean sides.
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Wednesday, January 21, 2015 9:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Embedding
After years of never winning the battle of paraffin on cassette edges after
embedding, we
After years of never winning the battle of paraffin on cassette edges after
embedding, we purchased a paraffin block trimmer. It saves time and the
stress on finger joints compared to scraping cassettes daily. No matter how
careful we were during embedding to keep excess paraffin off cassette ed
I have found really hot molds more difficult to work with but it is really
an individual preference.
What has worked for me is - put just enough paraffin in base mold to come
up to rim of embedding area. Embed tissue. Add just a little more
paraffin to bring it up to where plastic cassette top co
My first job the molds were kept room temp or cool and I didn't have to scrape
as much. My 2nd job they were kept hot and had to scrape all the time. Have
fun with it and test some cold, some room temp, some hot see if there is a
difference. The problem with too cool molds is it takes a bit lon
Mike,
For EM work we fix, scrape the plate into a microtube, centrifuge at 1800 rpm
for 10 min and then embed in Histogel or agar. Processing after that is as
usual. They look fine by EM. I'm sure they would look fine by paraffin
processing as well.
Tim Morken
Supervisor, Electron Microscopy
We do this all the time. Here is our protocol:
Agarose Pre-embedding Cell Cultures Pellets Collected in 15 ml. Tubes
Material:
• 10% Neutral Buffer Formalin
• 1X Dulbeccos PBS
• NuSieve GTG Low Melting Point Agarose: Lonza, Cat# 50080
• Corning 15 ml conical Centrifuge
We stumbled on Polyscientific's GemCut Pink Sapphire Parrafin and really like
it. Before that we used Paraplast and it was OK but our blocks cut so much
nicer with the GemCut.
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology
I use McCormick Paraplast Plus.
On Tuesday, November 12, 2013 8:14 AM, "Goins, Tresa" wrote:
Paraplast X-tra by McCormick.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark
Sent: Tues
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Tuesday, November 12, 2013 11:14 AM
To: Matthew D. Roark; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Embedding Paraffin
Paraplast X-tra by McCormick.
-Original Message-
From: histonet-boun
Paraplast X-tra by McCormick.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark
Sent: Tuesday, November 12, 2013 8:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding Pa
We use Richard-Allan Paraffin Type 9. Embed nice and cuts beautifully for our
lab.
dermatology
CONSULTANTS
skin. care. experts.
NOTICE OF CONFIDENTIALITY: The information in this email may be privileged and
confidential and protected from disclosure. If the reader of this message is
not
Maybe one of the used equipment vendors would consider a short-term lease with
option to buy.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MARCELLYN A.
STONE
Sent: Wednesday, March 13, 2013 7:14 AM
To:
John
Have you tried paraffin? I know that we have been able to section some plastic
devices with paraffin sections, it may be worth a try. Looks like the device
you are working with is compatable with xylene, we have found in some cases
that we need to use a xylene subsititute.
Liz
Elizabet
I started using the embedding beads several months ago as a quick way to track
who embedded what cassette. Each tech is assigned a number according to the
number on their microtome. I get the beads from Cancer Diagnostics. You can
probably find something similar at a craft store.
These come in s
I've never seen beads used for identifying individual embedders by
marking blocks with the beads. I never heard of Hama Beads (that's how
it's spelled), which I think may be called Perler Beads in the US, but
I never heard of them either. I think you can get them from Amazon.
Bob Richmond
Samurai
Donna,
Cancer Diagnostics has them, numbers 0 thru 9 and letters A thru E. Note,
however, that the "6" and "9" are identical! They run about 2 cents per bead
and come in bottles of 500.
They are flat on two sides so don't roll around. We've been using them for
about 6 months and everyone is h
I would get them from a craft shop or a bead shop - much cheaper!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property
Yes, cleaning your wells would be the next possible thing to try. Clean them
out with a little xylene (or paraguard) daily. Also if your embedding unit has
the removable wells, placing them upside down in the oven may be something to
try.
Toysha N. Mayer, MBA, HT (ASCP)
Instructor, Educatio
Sakura's Tissue-Tek
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weaver,
Stephanie
Sent: Tuesday, Sept
depressing article.
Amos
On Fri, Aug 26, 2011 at 1:00 PM,
wrote:
> Message: 13
> Date: Fri, 26 Aug 2011 11:36:15 -0400
> From: Bob Richmond
> Subject: [Histonet] Re: Embedding process etc.
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> >
> Content-Ty
18:07:26 +
> Subject: RE: [Histonet] RE: Embedding process improvement and
> competencyassessment
> CC: histonet@lists.utsouthwestern.edu;
> histonet-boun...@lists.utsouthwestern.edu; sdatt...@stormontvail.org
>
> How disgusting to hear about cheating. I recall that someone
Bill O'Donnell observes:
>>I once worked for a very demanding pathologist and because of his
>>expectations, the whole crew put out near perfection. Pathologists that are
>>"OK" with what they get, so long as they can make a diagnosis, are a huge
>>part of that problem.<<
I very much agree, th
estern.edu; D'Attilio, Shelley
Subject: RE: [Histonet] RE: Embedding process
improvementandcompetencyassessment
Tom, (and all those following this thread)
And we got that ;) perfection.
(Warning, long rant ahead... Rene's might have been longer, but he
spread it out over a number of pos
2011 1:21 PM
To: O'Donnell, Bill; Heath, Nancy L.; Jennifer MacDonald
Cc: Histonet Listserv (E-mail);
histonet-boun...@lists.utsouthwestern.edu; D'Attilio, Shelley
Subject: RE: [Histonet] RE: Embedding process
improvementandcompetencyassessment
Man did that bring back some memories. Bill a
: AW: [Histonet] RE: Embedding process improvement
andcompetencyassessment
Lucky me.
Being a MT in Austria I've learned also histotechniqe during education. ;)
So no discussions like this. But in reality people working in histologic or
chemical labs are of different species.
I
Lucky me.
Being a MT in Austria I've learned also histotechniqe during education. ;)
So no discussions like this. But in reality people working in histologic or
chemical labs are of different species.
I think managing and technical supervising are different skills. In small
teams both is in close
:
Subject: [Histonet] Re: Embedding process improvement...
Back to the question at hand,
You will need to engage the pathologists to provide information as to the
correct way to embed the skin specimens. If you have a dermatopathologist in
your practice, that person will need to provide the
Back to the question at hand,
You will need to engage the pathologists to provide information as to the
correct way to embed the skin specimens. If you have a dermatopathologist in
your practice, that person will need to provide the information about how
he/she dissects it, what he/she wants, a
riginal Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heath,
Nancy L.
Sent: Thursday, August 25, 2011 11:39 AM
To: Jennifer MacDonald
Cc: Histonet Listserv (E-mail);
histonet-boun...@lists.utsouthwestern.edu; D'Attilio,Shelley
Joe"
To: Bea DeBrosse-Serra ; Jennifer MacDonald
; "Heath, Nancy L."
Cc: Histonet Listserv (E-mail) ;
"histonet-boun...@lists.utsouthwestern.edu"
; "D'Attilio, Shelley"
Sent: Thu, August 25, 2011 1:07:26 PM
Subject: RE: [Histonet] RE: Embedding p
tonet-boun...@lists.utsouthwestern.edu; D'Attilio, Shelley
Subject: RE: [Histonet] RE: Embedding process improvement
andcompetencyassessment
How disgusting to hear about cheating. I recall that someone was
supposed to sign off as a witness that the applicant had done the work
themselves. I spent months acquiring tis
D'Attilio, Shelley
Subject: RE: [Histonet] RE: Embedding process improvement and
competencyassessment
I heard of a lot of cheating as well. People paid others to do the blocks and
staining. How good does it do? In the end, these people are cheating
themselves. Very sad!
Beatrice DeBrosse-Ser
tern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heath,
Nancy L.
Sent: Thursday, August 25, 2011 11:39 AM
To: Jennifer MacDonald
Cc: Histonet Listserv (E-mail);
histonet-boun...@lists.utsouthwestern.edu; D'Attilio,Shelley
Subject: RE: [Histonet] RE: Embedding process improvementand
ct of having the HT/HTL behind
> your name.
>
>
>
> From: Jennifer MacDonald [mailto:jmacdon...@mtsac.edu]
> Sent: Thursday, August 25, 2011 10:58 AM
> To: Heath, Nancy L.
> Cc: Histonet Listserv (E-mail);
> histonet-boun...@l
...@lists.utsouthwestern.edu;
D'Attilio, Shelley
Subject: RE: [Histonet] RE: Embedding process improvement and
competencyassessment
I fail to see the correlation of a non HT person supervising the Histology
lab and the lack of a practical exam for HT/HTL staff. One of the issues
that Shelley brought up wa
To: nhe...@lifespan.org
> From: jmacdon...@mtsac.edu
> Date: Thu, 25 Aug 2011 07:57:46 -0700
> Subject: RE: [Histonet] RE: Embedding process improvement and
> competencyassessment
> CC: histonet@lists.utsouthwestern.edu;
> histonet-boun...@lists.utsouthwestern.edu; sdatt...@storm
lley;
Podawiltz, Thomas
Subject: RE: [Histonet] RE: Embedding process improvement and
competencyassessment
I fail to see the correlation of a non HT person supervising the
Histology lab and the lack of a practical exam for HT/HTL staff. One of
the issues that Shelley brought up was the staff
x27;Attilio, Shelley" , "Podawiltz, Thomas"
, "Histonet Listserv (E-mail)"
cc
Subject
RE: [Histonet] RE: Embedding process improvement and competencyassessment
This is exactly why the powers that be should have NEVER gotten rid of
the practical portion of the HT/HTL
Well said.
"Johnson, Teri"
Sent by: histonet-boun...@lists.utsouthwestern.edu
08/25/2011 07:39 AM
To
Histonet
cc
Subject
[Histonet] Re: Embedding process improvement and competency assessment
Dear Shelly,
I would work for you without reserve.
I have managed both cyt
Dear Shelly,
I would work for you without reserve.
I have managed both cytometry and Electron Microscopy successfully, and I
cannot do either technique. However, I understand enough about it to make sound
decisions and empower my people enough that it works well. It is possible to do
well if d
Sent: Thursday, August 25, 2011 9:45 AM
To: Podawiltz, Thomas; Histonet Listserv (E-mail)
Subject: [Histonet] RE: Embedding process improvement and
competencyassessment
Hi Tom,
Thank you for your kind words. I am off the bench almost completely. I
can work in the gross room in a pinch and my cou
Hi Tom,
Thank you for your kind words. I am off the bench almost completely. I can
work in the gross room in a pinch and my counting skills are excellent, so I
can always file slides and block if an emergency arises:) I occasionally cover
a bench in Chemistry as well, but my staff is all pret
sing one paraffin type is
> fine, all personal preference. We could go on forever with this!!
>
>
> I am not endorsing any paraffin on here, used many different paraffins and
> each work as anticipated.
>
> Thanks,
>
> Tim
>
>
>
>
> Sent: Monday, July 11,
lower, but usually higher) is a mistake.
Select a paraffin adequate for your subject and use it for both the
infiltration and the embedding.
René J.
--- On Mon, 7/11/11, amitapan...@torrentpharma.com
wrote:
From: amitapan...@torrentpharma.com
Subject: Re: [Histonet] Re: Embedding Media
To: &quo
any feed back on Richard Allan product.
>
> Amita
>
>
> From: "Tim Higgins"
> To:
> Date: 07/08/2011 11:34 PM
> Subject:[Histonet] Re: Embedding Media
> Sent by:histonet-boun...@lists.utsouthwestern.edu
>
>
>
> Hi Paula,
an product.
Amita
From: "Tim Higgins"
To:
Date: 07/08/2011 11:34 PM
Subject: [Histonet] Re: Embedding Media
Sent by:histonet-boun...@lists.utsouthwestern.edu
Hi Paula,
Obviously there are no "compression free" paraffin's on the market. Look
f
Hi Paula,
Obviously there are no "compression free" paraffin's on the market. Look
for a paraffin with a higher polymer content, this makes the paraffin firmer
and less likely to compress. Other factor for into section compression but
paraffin is a good place to start.
The higher polymer paraff
There is also a company that makes great embedding presses called
Histopress. I use them and love them! Check Histopress.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
...@lists.utsouthwestern.edu]On Behalf Of Horn,
Hazel V
Sent: Monday, April 11, 2011 11:06 AM
To: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; Gale Limron;
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: embedding forceps
These are the same ones we like. We like the 7" ones.
Hazel Horn
Hazel Hor
ern.edu
Subject: [Histonet] RE: embedding forceps
I like these:
You can get them in different lengths.5 1/2" or 7"
http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/
Jeanine Bartlett, BS, HT(ASCP
I like these:
You can get them in different lengths.5 1/2" or 7"
http://www.leica-microsystems.com/products/total-histology/consumables/autopsy-dissection/dissection/details/product/512-14cm-ergonomic-1/
Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
I
4 Suite 100
Austin, Texas 78744
[DEL: (512)386-5107
:DEL]
Original Message --------
Subject: [Histonet] RE: embedding beads/tissue marking dyes
From: Maria Katleba <[1]Maria.Ka= tl...@stjoe.org>
Date: Tue, October 05, 2010 2:40 pm
To: Jacqueline Farnsworth
When you place dye on any tissue, make sure you pipette some bouins on to the
tissue... Apparently the bouins "sets" the stain... afterwards dab the tissue
with paper towel to sop up the excess dye/bouins.
Some dyes are better than others, but try the bouins first
Call me or email me directly, I
ton
[flna...@texaschildrens.org]
Sent: Thursday, September 02, 2010 11:38 AM
To: 'Madary, Joseph'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: embedding center just did alot of research!
Even over Tissue Tec
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[
Even over Tissue Tec
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph
Sent: Thursday, September 02, 2010 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding center ju
I am not sure if you are referring to bubble between the tissue and the bottom
of the molds, or bubbles causing holes between the tissue and the cassette,
causing the block to "collapse". The previous would probably have something to
do with the paraffin feeding into the block, perhaps there i
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