Only 40 minutes in formalin is a problem.
Increase the formalin time to several hours and this should suffice for small
2-3mm punch biopsies of skin.
For larger skin sections the alcohol, xylene and wax times will need to be
tripled at least (as well as fixing the skins for longer (3-4 hours on
i agree with Liz, the fat in the skin requires a little= longer
processing than other soft tissues especially human, animals d= on't
have as much fat and should be processed on a shorter schedule to keep
them from becoming too hard and dehydrated.
&= nbsp;
nt' ; "histonet@lists.utsouthwestern.edu"
Sent: Friday, November 4, 2011 1:37 PM
Subject: [Histonet] RE: processing program for biopsies
In my personal opinion I think the processing cycle is too short. I know that
skin biopsies are tiny but we process all skin samples on longer proce
What does look bad mean? In what way do they look bad? Do they cut well?
What tissue processor do you use?
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com
-Original Message-
Fr
In my personal opinion I think the processing cycle is too short. I know that
skin biopsies are tiny but we process all skin samples on longer processing
cycles. Granted I'm in research but we use 45 minutes to 1 hour per station
for all of our skin samples and they cut just fine.
Liz
Elizab