Jim, unless they've changed methodologies since I left clinical, they process
all DIF for renal biopsies for cryosectioning.
I'm very aware of the different methods for quenching autofluorescence, and I
know that what works for some samples doesn't work entirely for all.
Additionally, what may
Original Message-
From: WILLIAM DESALVO [mailto:wdesalvo@hotmail.com]
Sent: Thursday, August 27, 2009 12:51 PM
To: Johnson, Teri; histonet
Subject: RE: [Histonet] Re: DIF tissue in GLUT
I suggest you use a counterstain for your IF to reduce the autofluoresence.
Evans Blue - the prod
:47 -0500
> Subject: [Histonet] Re: DIF tissue in GLUT
>
> Anne,
>
> Tissue that has been fixed in glutaraldehyde has very, VERY bright
> autofluorescence. Unless there is some way to minimize this (none that I'm
> aware of), your immunofluorescence will be impossible to
Anne,
Tissue that has been fixed in glutaraldehyde has very, VERY bright
autofluorescence. Unless there is some way to minimize this (none that I'm
aware of), your immunofluorescence will be impossible to read over the
background signal.
Teri Johnson, HT(ASCP)QIHC
Managing Director Histology F
