Good point by Teri Johnson.
I don't decalcify, thus my results may well be compromised by this
procedureand, sure, the bone is disrupted.
I am only interested in CNS/PNS so, I don't worry about bone/cartilage
disruption.
I fix in std 10% NBF.
Imho, it is better to overfix rather than to
Should be no difference ( no tricks)
Have a look here for some images:
http://www.immunoportal.com/
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson Centre for Age-Related Diseases
School of Biomedical Sciences
King's College London
Guys Campus
London SE1 1UL
Tel.020 7848 6810
fax 020
The differences might be due to decalcification and/or differences in fixation.
I presume you are decalcifying the fish? Are you using EDTA or a formic acid
decalcifier? Are they fixed in 10%NBF (or equivalent) for roughly the same
amount of time as your human samples? Or are you using a
