r 12, 2021 5:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Reprocessing Protocol
Hi Curt,
We have used Taggart's Method quite successfully in the past for poorly
processed tissues. You can find it online. The isotonic saline may help to
rehydrate your tissues.
1.
Curt Tague)
--
Message: 1
Date: Tue, 12 Oct 2021 15:14:54 +
From: Curt Tague
To: "histonet@lists.utsouthwestern.edu"
Subject: [Histonet] reprocessing tissue
Message-ID:
Content-Type: text/plain; charset="us
Are these FFPE tissue? We usually just do the program in reverse order by
deparaffinizing, 100% alc., 95% alc., 70% alc., water, formalin.
Jose
-Original Message-
From: Curt Tague
Sent: Tuesday, October 12, 2021 11:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet
I have a problem... some tissue got processed very poorly, there was water in
the system somewhere and a few blocks just look burnt.. the nuclei are faint
and cloudy, no detail at all. I've tried the process of rehydrating with the
30% formaldehyde, glycerol and sodium acetate solution but they
Hello,
I am wondering if anyone has had any success with reprocessing biopsy
tissue, and if so, what process you used. We recently had an issue where water
was reintroduced into the tissue during the dehydration step due to an
incorrect alcohol percentage being entered. The Peloris
for iOS<https://aka.ms/o0ukef>
From: Cassie P. Davis via Histonet
Sent: Tuesday, September 3, 2019 1:54:13 PM
To: Perl , Alison
Cc: 'Cassie P. Davis via Histonet'
Subject: Re: [Histonet] Reprocessing tissue
Thank to those who responded so quickly. It h
stonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, September 03, 2019 1:32 PM
To: histonet
Subject: [EXTERNAL] [Histonet] Reprocessing tissue
Has anyone had success with just melting down unfaced, underprocessed blocks
for reprocessing and sticking them in NBF for processing without
@lists.utsouthwestern.edu]
Sent: Tuesday, September 03, 2019 1:32 PM
To: histonet
Subject: [EXTERNAL] [Histonet] Reprocessing tissue
Has anyone had success with just melting down unfaced, underprocessed blocks
for reprocessing and sticking them in NBF for processing without taking them
through
Has anyone had success with just melting down unfaced, underprocessed blocks
for reprocessing and sticking them in NBF for processing without taking them
through the clean cycle? The senior tech is doing this and I am concerned about
our processor getting paraffin in the NBF and other things.
Here is very good free e-book:
http://www.leicabiosystems.com/fileadmin/biosystems/PDF/95.9890_Rev_C_Difficult_Blocks_and_Reprocessing.pdf
--
Maxim Peshkov,
Russia,
Taganrog.
mailto:maxim...@mail.ru
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. Callis
HTL/HT/MT(ASCP)
-Original Message-
From: Mca Werdler via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, April 07, 2016 9:02 AM
To: Charles Riley <cri...@dpspa.com>
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Reprocessing
What i wo
What i would do, melt the blocks you made.
1. Put them back in paraffin for one hour
2. Put them back in another paraffin or one hour
3. Put them in xylene for one hour
4. Put them in another xylene for one hour
5. Put them in another xylene for one hour
6. Put them back in paraffin for one hour
Hello all,
We had an issue with our processor the other night. Someone accidentally
put 100% alochol into the last xylene station. The tissue sections were
difficult to cut and or were unreadable. What is the best way to reprocess
them? They are mostly small GI biopsies and only two larger
Has anyone re-processed a small pc. of tissue before? if so how, do you do it?
In general, how are you re-processing tissue?
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Does anyone have a procedure for reprocessing tissue? We need to reprocess a
breast and lymph node
sections. They are squishy in the middle. They were probably cut to thick and
not fixed long enough but you know the Pathologist wants them as soon as
possible. Any ideas will be appreciated.
Just melt the paraffin off and pat dry, process from formalin normally. Just
did it yesterday, works great.
Laurie
--Original Message--
From: nancy monroe elainemon...@live.com
Date: Fri Aug 14, 2009 -- 10:59:08 AM
To: histonet@lists.utsouthwestern.edu
Subject:[Histonet
Put you tissues in the tissue processor through the cleaning cycle, and
reprocess them again starting in the alcohols.
René J.
--- On Fri, 8/14/09, nancy monroe elainemon...@live.com wrote:
From: nancy monroe elainemon...@live.com
Subject: [Histonet] reprocessing tissue
To: histonet
wrote:
From: kristen arvidson arvidsonkris...@yahoo.com
Subject: [Histonet] reprocessing
To: histonet histonet@lists.utsouthwestern.edu
Date: Tuesday, February 3, 2009, 1:13 PM
Hello,
What are people doing these days when they have to reprocess fatty tissue
:
From: kristen arvidson arvidsonkris...@yahoo.com
Subject: [Histonet] reprocessing
To: histonet histonet@lists.utsouthwestern.edu
Date: Tuesday, February 3, 2009, 1:13 PM
Hello,
What are people doing these days when they have to reprocess fatty tissue
PROTECTED] On Behalf Of Pumla
Pamla-Gutter
Sent: Thursday, November 13, 2008 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reprocessing HematoxylinEosin tongue slides.
Hello,
Could anyone help me with a protocol for re staining HE formalin-fixed
tissue sections?
I have some HE
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