Re: [Histonet] Routine H E stain,

2011-07-07 Thread SHANE NELSON
; histonet histonet-requ...@lists.utsouthwestern.edu Sent: Wed, July 6, 2011 9:22:38 PM Subject: [Histonet] Routine H E stain, Hello Histonetters, I am from toxicopathology lab where we perform HE on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin

Re: [Histonet] Routine H E stain,

2011-07-07 Thread SHANE NELSON
Sent: Wed, July 6, 2011 9:22:38 PM Subject: [Histonet] Routine H E stain, Hello Histonetters, I am from toxicopathology lab where we perform HE on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W

Re: [Histonet] Routine H E stain,

2011-07-07 Thread kgrobert
Amita, We are a neurotoxicology lab that also provides histology services for other researchers inside outside Rutgers, and we use Gill's #3 hematoxylin for our routine HEs on mouse and rat tissues. No fading that I have noticed in all the years that I have been here. We get ours from Thermo

Re: [Histonet] Routine H E stain,

2011-07-07 Thread Rene J Buesa
It seems that your mounting medium is acid. Try to correct that rather than changing the staining. René J. --- On Thu, 7/7/11, amitapan...@torrentpharma.com amitapan...@torrentpharma.com wrote: From: amitapan...@torrentpharma.com amitapan...@torrentpharma.com Subject: [Histonet] Routine H E

RE: [Histonet] Routine H E stain,

2011-07-07 Thread Rittman, Barry R
; amitapan...@torrentpharma.com Subject: Re: [Histonet] Routine H E stain, It seems that your mounting medium is acid. Try to correct that rather than changing the staining. René J. --- On Thu, 7/7/11, amitapan...@torrentpharma.com amitapan...@torrentpharma.com wrote: From: amitapan

[Histonet] Routine H E stain,

2011-07-06 Thread amitapandey
Hello Histonetters, I am from toxicopathology lab where we perform HE on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result