I have never (3 different places, 30+ years) heard of requiring special stains
to be performed under a hood. Certainly, there are a few reagents that might
need benefit from a hood (concentrated Ammonium Hydroxide) but generally, they
are performed on an open counter next to a sink. If you
25+ years in Histology, half the places I worked had vents on the wall near the
countertop where we did specials, otherwise it was on the counterclosest to the
sink. The only times we were time doing anything under a hood was for frozen
section staining because of the fresh tissue, mixing
romuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, August 21, 2018 7:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special stains an
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, August 21, 2018 9:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special stains and hoods
CAUTION: This email originated from outside of Bellin. Do not click on any
links or open any attachments unless you recognize the sender and know
I would like to know how many people perform (manual) routine special stains
under a hood. We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci -
but we do them on the counter, not under a hood. We have been told the
staining needs to be done under a hood, but I have never done that in
Hello all!
Is it a CAP requirement to validate special stains just as you would IHC??
Thanks in advance.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hello,
I was wondering if any of you might be able to help me out. I'm searching for
samples of a template/ form validation sheet that I can use for documentation
and CAP inspection for control blocks for special stains. Thank you in advance
___
Morning all!
Does anyone have experience doing specials, esp. Gram stains, on the Sakura
Prisma and/or the Leica ST5020 multistainer?
Thanks much and Happy Memorial Day tomorrow!
Jeanine H. Sanders
Infectious Diseases Pathology Branch
Centers for Disease Control and Prevention
1600 Clifton
Hello fellow histonetters,
I am new to working with zinc formalin and am having issues with staining
special stains. Does anyone have any advice to troubleshoot this problem? I am
particularly having trouble with AB/PAS and PAS stains. Any assistance is
appreciated.
Thank you.
Haley
Haley
Hi
I think it was about six or seven years ago I had come across a company that
made a 'promotional' laminated poster of widely used special stains that
they had given to customers to show new techs and students how stains should
look. I've had no luck in searching the internet so I'm hoping
Our lab would like to know how you handle the quality issue of verifying that
the control material you use for special stains is what it's supposed to be.
For example: how do you confirm that bile in a piece of liver used as a Halls
control is actually bile?
We use controls for bacteria or
We are a research lab and occasionally do a few special stains (Masson
Trichrome, PAS, Picro-Sirius Red, Safranin O).
We usually purchase our stains ready made (or at least the components).
However, some of these stains are very expensive, namely the reagents for Masson
Trichrome. We just
I couldn't agree with you more, Liz! I am from a GLP lab as well and we
have to undergo validation for every single new piece of equipment we
acquire otherwise we are not considered GLP-compliant. Who is to say
that your new stainer is going to stain slides in the same manner as
your old
We are switching automated special stain equipment to a different vendor and am
wondering if we have to validate all of the special stains we do. I am from
the old school and the validation process, other than IHC, is not my forte!!
Any advice would be appreciated, so, thanks ahead of time!!
' histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] Special stains
We are switching automated special stain equipment to a different vendor
and am wondering if we have to validate all of the special stains we do. I
am from the old school and the validation process, other than IHC
because of changing the
auto stainer, as long as the procedure is the same.
René J.
--- On Tue, 1/5/10, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote:
From: Webb, Dorothy L dorothy.l.w...@healthpartners.com
Subject: [Histonet] Special stains
To: 'histonet@lists.utsouthwestern.edu' histonet
validation because of changing the
auto stainer, as long as the procedure is the same.
René J.
--- On Tue, 1/5/10, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote:
From: Webb, Dorothy L dorothy.l.w...@healthpartners.com
Subject: [Histonet] Special stains
To: 'histonet
...@yahoo.com
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/05/2010 11:38 AM
To
'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu,
Dorothy LWebb dorothy.l.w...@healthpartners.com
cc
Subject
Re: [Histonet] Special stains
Dorothy:
I would NOT do it.
If you do not change
] On Behalf Of
kim.dona...@bhcpns.org
Sent: Tuesday, January 05, 2010 10:28 AM
To: Rene J Buesa
Cc: 'histonet@lists.utsouthwestern.edu';
histonet-boun...@lists.utsouthwestern.edu; Dorothy LWebb
Subject: Re: [Histonet] Special stains
In the end it is still a new instrument. It should be validated
...@lists.utsouthwestern.edu
Subject: Re: [Histonet] Special stains
Subject
[Histonet] Special stains
We are switching automated special stain equipment to a different vendor
and am wondering if we have to validate all of the special stains we do. I
am from the old school and the validation process, other than
@lists.utsouthwestern.edu; Rosa Fields
Subject: RE: [Histonet] Special stains and microwave
Hi Rosa,
Excuse me if I am wrong, but I think Bill is referring to using a
microwave for special stains, and you are referring to a Microwave
Tissue Processor.
I have used an oven set at a higher temperature (85 -110
Greetings Histonetters,
We have finally come to the point where we need to fish or cut bait in
relation to purchasing a laboratory approved microwave and venting it.
While I think it would be cool to have one, I'm wondering if the cost
is justified to do a handful of special stains that could
, is strictly prohibited.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Thursday, August 27, 2009 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special stains
--- On Thu, 8/27/09, Rosa Fields rfie...@gidocs.net wrote:
From: Rosa Fields rfie...@gidocs.net
Subject: RE: [Histonet] Special stains and microwave
To: O'Donnell, Bill billodonn...@catholichealth.net,
histonet@lists.utsouthwestern.edu
Date: Thursday, August 27, 2009, 2:26 PM
Bill,
I can highly
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