;t.
Ray (still in, whoever would have guessed, once again rainy Seattle)
- Original Message -
From: "M.O."
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, April 15, 2014 5:59:51 PM
Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block?
Hello Histonet!
;t.
Ray (still in, whoever would have guessed, once again rainy Seattle)
- Original Message -
From: "M.O."
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, April 15, 2014 5:59:51 PM
Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block?
Hello Histonet!
Hello Histonet!
I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from
vector. The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). I
have never stained with anything like this, so I ran a test.
I deparaffinized, blocked with NGS, incubated overnight at 4C with the
dilu