RE: [Histonet] Staining FFPE with biotinylated SNA - how to block?

2014-04-16 Thread gayle callis
;t. Ray (still in, whoever would have guessed, once again rainy Seattle) - Original Message - From: "M.O." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 15, 2014 5:59:51 PM Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hello Histonet!

Re: [Histonet] Staining FFPE with biotinylated SNA - how to block?

2014-04-16 Thread koellingr
;t. Ray (still in, whoever would have guessed, once again rainy Seattle) - Original Message - From: "M.O." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 15, 2014 5:59:51 PM Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hello Histonet!

[Histonet] Staining FFPE with biotinylated SNA - how to block?

2014-04-15 Thread M.O.
Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with NGS, incubated overnight at 4C with the dilu