I have used down to -30 a few times for research samples, mainly to try and
get stuff to cut better rather than that is what was requested. Everything
gets very brittle at that temperature, including your hands after a short
while.
Never -60 though!
Maybe that is just for the peltier freezing mec
Thank you Paul.
Colleen
On Thursday, May 19, 2022, P Sicurello wrote:
> Cryo-electron microscopy cuts thin sections at temperatures colder than
> that. It is used to preserve antigenicity in certain situations.
>
> However, a cryostat is not used. An ultramicrotome fitted with specific
> cryo
Cryo-electron microscopy cuts thin sections at temperatures colder than that.
It is used to preserve antigenicity in certain situations.
However, a cryostat is not used. An ultramicrotome fitted with specific cryo
attachments is used.
Sincerely,
Paula Sicurello
Sent from my iPhone
> On
FollowingI am in research and I have never cut this cold.
Colleen Forster
On Wed, May 18, 2022 at 2:04 PM Ken Marzinsky via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Can anyone tell me why research labs often require cryostat cutting head
> temperatures down to -60C?
>
> Ken
> D
Can anyone tell me why research labs often require cryostat cutting head
temperatures down to -60C?
Ken
Durham, NC
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