Re: [Histonet] Super cold cryostat cutting head temperatures

2022-05-20 Thread Caroline Miller via Histonet
I have used down to -30 a few times for research samples, mainly to try and get stuff to cut better rather than that is what was requested. Everything gets very brittle at that temperature, including your hands after a short while. Never -60 though! Maybe that is just for the peltier freezing mec

Re: [Histonet] Super cold cryostat cutting head temperatures

2022-05-19 Thread Colleen Forster via Histonet
Thank you Paul. Colleen On Thursday, May 19, 2022, P Sicurello wrote: > Cryo-electron microscopy cuts thin sections at temperatures colder than > that. It is used to preserve antigenicity in certain situations. > > However, a cryostat is not used. An ultramicrotome fitted with specific > cryo

Re: [Histonet] Super cold cryostat cutting head temperatures

2022-05-19 Thread P Sicurello via Histonet
Cryo-electron microscopy cuts thin sections at temperatures colder than that. It is used to preserve antigenicity in certain situations. However, a cryostat is not used. An ultramicrotome fitted with specific cryo attachments is used. Sincerely, Paula Sicurello Sent from my iPhone > On

Re: [Histonet] Super cold cryostat cutting head temperatures

2022-05-18 Thread Colleen Forster via Histonet
FollowingI am in research and I have never cut this cold. Colleen Forster On Wed, May 18, 2022 at 2:04 PM Ken Marzinsky via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Can anyone tell me why research labs often require cryostat cutting head > temperatures down to -60C? > > Ken > D

[Histonet] Super cold cryostat cutting head temperatures

2022-05-18 Thread Ken Marzinsky via Histonet
Can anyone tell me why research labs often require cryostat cutting head temperatures down to -60C? Ken Durham, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet