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From: Carrie Disbrow via Histonet
Sent: Monday, 14 June 2021 12:04
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Unstained slides pr
Hi! Current lab is cutting extra slides for IHC and putting in 60 degree oven
for 45 mins to dry. If IHC is ordered a precut control and the already baked
slides are again put in the 60 degree oven for 45 mins. Sometimes the control
is cut and added to the dry slide or a separate slide. So,
How about frozen sections cut for immunofluorescence stored at -20?
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is
Hi
Depends on what you mean by cryosections.
Unfixed/fixed?
Stored at RT, 4C, -20C, -80C.
Stored dry or in glycerol
So many variables!
My opinion is to store blocks and cut sections as required.
Least variables.
Sure, one loses some tissue everytime one cuts anewa good thing.
It IS
: Hobbs, Carl [mailto:carl.ho...@kcl.ac.uk]
Sent: Tuesday, 4 September 2018 4:54 AM
To: histonet
Subject: Re: [Histonet] Unstained slides
I agree: cut only the sections needed.
Saves space.
Sure, you lose several sections of tissue when cutting more sections.
That is acceptable because
I agree: cut only the sections needed.
Saves space.
Sure, you lose several sections of tissue when cutting more sections.
That is acceptable because, if this "oxidation" theory is true, then the
initial sections will be no good.
However, careful organisation of exptl procedure before actual
Sunday, August 19, 2018 2:09 PM
> To: Frazier, John; Terri Braud
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Unstained slides
>
> This is an email from Outside HHC. USE CAUTION opening attachments or links
> from unknown senders.
>
> Everything has been
(860) 545-2204 Fax
>
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Sunday, August 19, 2018 2:09 PM
> To: Frazier, John; Terri Braud
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Unstained slides
tern.edu]
Sent: Sunday, August 19, 2018 2:09 PM
To: Frazier, John; Terri Braud
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unstained slides
This is an email from Outside HHC. USE CAUTION opening attachments or links
from unknown senders.
Everything has been pointed out is correct
Good Afternoon Listers,
Thanks to all who answered my question about retention of unstained
slides. I had several people weigh in on the topic and we have created an
unstained slide policy based on their advice and the literature that was
kindly sent to me.
The consensus is antigenicity
Hi Everyone!
I have seen unstained slides save a patient from re-biopsy many times.
Usually it will be a case where a patient has a known diagnosis, like lung
cancer. In these types of cases after diagnosis molecular testing (and IHC
for PD-L1) is usually ordered. There have been countless
To expand on what we do at our research lab; we use 6 months as a standard
maximum age of unstained slides. Also in the staining protocol for each
antibodyl we have a specific shelf life for the diluted Antibody and a
maximum age of unstained slides.
Jamie
I agree with Jamie
Only a few Ags are "oxidised" ( that's the term used, I recall) but, don't let
it be YOUR protein of interest.
If you really are concerned, cut fresh sections and immunostain along with your
stored sections.
Imho: cut as few sections as you need.
Store any unused at 4C
on Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> Today's Topics:
> 7. Re: Unstained slides - how long are they good for?
> (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "
ained slides - how long are they good for?
> (Morken, Timothy)
>
> Message: 7
> Date: Fri, 17 Aug 2018 15:16:00 +
> From: "Morken, Timothy"
> To: P Sicurello
> Subject: Re: [Histonet] Unstained slides - how long are they good for?
>
>
> Paula, since
I’m a histology workflow consultant that visits many AP laboratories
each year. Almost every laboratory has a different retention policy.
The average of most laboratories is to hold onto unstained slides for
three weeks after final sign out. Typically the unstained slide can be
held for a long
, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:
7. Re: Unstained slides - how long are they good for?
(Morken, Timothy)
Message: 7
Date: Fri, 17 Aug 2018 15:16:00 +
From: "Morken, Timothy"
To: P Sicurello
Subject: Re: [Histonet] Unstai
a Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, August 16, 2018 4:49 PM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?
Hello My Fellow Histologists,
Happy Friday Eve.
The question has come up.. How long are *unstained* slides good for?
nohistochemistry & Molecular Morphology, 26(7), 489-494.
-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, 17 August 2018 9:49 AM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?
Hello My Fellow Histo
It depends on the stability of the protein and fixation, some are stable
for a week some for years. We use 6 months as a standard.
Jamie
On August 16, 2018 4:59:34 PM P Sicurello via Histonet
wrote:
> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up.. How
Hello My Fellow Histologists,
Happy Friday Eve.
The question has come up.. How long are *unstained* slides good for?
Not for H but tests like IHC and molecular testing. These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.
Please let me
] On Behalf Of Beth Brinegar
Sent: Wednesday, August 28, 2013 4:47 PM
To: Martha Ward-Pathology
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unstained slides
Martha,
I have had experience in three different labs. The unstained slides can
pile up quickly, especially when you are using
We are looking at ways to improve our work processes, save time and labor and
reduce costs, all while maintaining patient quality...as we all are of course.
During our conversations the subject of cutting unstained slides has come up
and we are looking for bench marking data to see if we are
Martha,
I have had experience in three different labs. The unstained slides can
pile up quickly, especially when you are using charged slides that are
just in case slides for sendouts, IHC, etc. For core biopsies (breasts,
fine needles, prostate, etc.) we cut levels and put up at least five of
Hello Histonetters,
Recently we have seen an increase number of unstained slides requested from
outside facilities. We do not release our blocks, so unstained slides are
the only option for any facility to obtain our tissue. Currently we do not
charge for these slides, but I am rethinking that
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] unstained slides
Hello Histonetters,
Recently we have seen an increase number of unstained slides requested
from
outside facilities. We do not release our blocks, so unstained slides
are
the only option for any facility to obtain our tissue
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, June 28, 2010 10:55 AM
To: histonet@lists.utsouthwestern.edu;
rick.garnh...@memorialhealthsystem.com
Subject: SPAM-LOW: Re: [Histonet] Unstained Slides
We bake our unstains at 60 degrees C
...@memorialhealthsystem.com
Subject: [Histonet] Unstained Slides
To: histonet@lists.utsouthwestern.edu
Date: Monday, June 28, 2010, 11:54 AM
Histoland, How is everyone storing/filing unstained slide. And how long are
they good for to use for immunohistochemistry.
Rick Garnhart HT(ASCP)
Memorial
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